Cell No. : Cell Name RCB0762 : MT-9  update : 2022/03/23
Comment			Rat cell line derived from malignant fibrous histiocytoma. Possessing characteristics of histiocytes.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Yamate, Jyoji
		Originator	Yamate, Jyoji
		Year of deposit	1992
		Original cell	MT-P
		Animal	_rat < Mammals
		Strain name	F344/DuCrj
		Genus	Rattus
		Species	norvegicus
		Gender	Male
		Age at sampling	15 months
		Tissue	subcutis of the head
		Disease name	Malignant Fibrous Histiocytoma
		Metastatic ability	No
		Classification	cancer
		Year of origin	1986
		Lifespan	infinite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_5338
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		DMEM (low glucose) + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Animal PCR		OK
		Isozyme		LD, NP
Images			deposit info	lot info
Reference information		Reference	16
		User's Publication	0

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Reference

3495  Takashi Kotera 1 , Chisa Katou-Ichikawa, Anusha Hemamali Tennakoon, Miyuu Tanaka, Natsuki Tanaka, Takeshi Izawa, Mitsuru Kuwamura, Jyoji Yamate   Rat malignant fibrous histiocytoma (MFH)-derived cloned cell lines (MT-8 and MT-9) show different differentiation in mesenchymal stem cell lineage  Eep Toxicol Pathol  2015  67(10):499-507  PubMed ID: 26208870   DOI: 10.1016/j.etp.2015.07.004

3493  Ryo Yano, Hossain M Golbar, Takeshi Izawa, Osamu Sawamoto, Mitsuru Kuwamura , Jyoji Yamate  Participation of bone morphogenetic protein (BMP)-6 and osteopontin in cisplatin (CDDP)-induced rat renal fibrosis  Eep Toxicol Pathol  2015  67(2):99-107  PubMed ID: 25467747   DOI: 10.1016/j.etp.2014.10.002

3490  Takeshi Izawa, Takashi Horiuchi, Machi Atarashi, Mitsuru Kuwamura, Jyoji Yamate   Anti-fibrotic Role of miR-214 in Thioacetamide-induced Liver Cirrhosis in Rats  Toxicol Pathol  2015  43(6):844-51  PubMed ID: 25755099   DOI: 10.1177/0192623315573587

3494  Jyoji Yamate, Keiko Ogata, Takahiro Yuasa, Mitsuru Kuwamura, Shigeo Takenaka, Daijirou Kumagai, Kazuyuki Itoh, Jonathan LaMarre  Adipogenic, osteogenic and myofibrogenic differentiations of a rat malignant fibrous histiocytoma (MFH)-derived cell line, and a relationship of MFH cells with embryonal mesenchymal, perivascular and bone marrow stem cells  Eur J Cancer  2007  43(18):2747-56  PubMed ID: 18036812   DOI: 10.1016/j.ejca.2007.10.021

3491  Jyoji Yamate, Masanori Kuribayashi, Mitsuru Kuwamura, Takao Kotani, Kikumi Ogihara  Differential immunoexpressions of cytoskeletons in renal epithelial and interstitial cells in rat and canine fibrotic kidneys, and in kidney-related cell lines under fibrogenic stimuli  Eep Toxicol Pathol   2005  57(2):135-47.  PubMed ID: 16325524   DOI: 10.1016/j.etp.2005.07.001

2786  Tsunenari I, Yamate J, Iwaki M, Kuwamura M, Kotani T, Sakuma S  Angioarchitecture of tumors induced by two different cloned cell lines established from a transplantable rat malignant fibrous histiocytoma.  Microsc Microanal  2003  9:532-41  PubMed ID: 14750988   DOI: 10.1017/S1431927603030289

2788  Kumagai D, Yamate J, Tajima T, Tsukamoto Y, Yasui H, Kuwamura M, Kotani T, Sakuma S  Distribution of cells labelled by a monoclonal antibody (A3) against a cloned cell line derived from a rat malignant fibrous histiocytoma  J Comp Pathol  2001  123:77-87  PubMed ID: 11032659   DOI: 10.1053/jcpa.2000.0389

2783  Yamate J, Maeda M, Benn SJ, Laithwaite JE, Allan A, Ide M, Kuwamura M, Kotani T, Sakuma S, Lamarre J  Differential effects of transforming growth factor-beta1, a fibrogenic factor, on macrophage-like cells (HS-P) and myofibroblastic cells (MT-9) in vitro.  Toxicol Pathol  2001  29:483-91  PubMed ID: 11560254   DOI: 10.1080/01926230152500103

2784  Yamate J, Maeda M, Benn SJ, Laithwaite JE, Allan A, Ide M, Kuwamura M, Kotani T, Sakuma S, LaMarre J  Effects of lipopolysaccharide on a macrophage-like cell line (HS-P) from a rat histiocytic sarcoma.  J Comp Pathol  2001  125:15-24  PubMed ID: 11437512   DOI: 10.1053/jcpa.2001.0472

2787  Kumagai D, Yamate J, Tajima T, Kuwamura M, Kotani T, Sakuma S  Malignant fibrous histiocytoma (MFH) cells and macrophages/histiocytes have a common antigen recognized by a monoclonal antibody risen against a rat MFH-derived cloned cell line.  J Vet Med Sci  2001  63:333-5  PubMed ID: 11307938   DOI: 10.1292/jvms.63.333

2789  Tsunenari I, Yamate J, Sharma KD, Kawachi M, Sakuma S  Expressions of vascular endothelial growth factor and basic fibroblast growth factor in tumors induced by two different cloned cell lines established from transplantable rat malignant fibrous histiocytoma.  J Vet Med Sci  2000  62:699-705  PubMed ID: 10945286   DOI: 10.1292/jvms.62.699

2792  Yamate J, Tajima M, Shibuya K, Kuwamura M, Kotani T, Sakuma S, Takeya M  Heterogeneity in the origin and immunophenotypes of "histiocytic" cells in transplantable rat malignant fibrous histiocytoma.  J Vet Med Sci.  1996  58:603-609  PubMed ID: 8844594   DOI: 10.1292/jvms.58.603

2790  Yamate J, Tajima M, Shibuya K, Kuwamura M, Kotani T, Sakuma S  Phenotypic changes in lipopolysaccharide-treated cloned cells derived from transplantable rat malignant fibrous histiocytoma.  J Vet Med Sci  1996  58:1017-20  PubMed ID: 8916004   DOI: 10.1292/jvms.58.10_1017

2791  Yamate J, Tajima M, Shibuya K, Kuwamura M, Kotani T, Sakuma S  Phenotypic modulation in cisplatin-resistant cloned cells derived from transplantable rat malignant fibrous histiocytoma.  Pathol Int  1996  46:557-67  PubMed ID: 8893224   DOI: 10.1111/j.1440-1827.1996.tb03654.x

2793  Yamate J, Tajima M, Shibuya K, Saitoh T  Enhancement of lipid phagocytosis by cloned cells derived from rat malignant fibrous histiocytoma under hyperlipemic conditions.  Pathol Int  1994  44:735-44  PubMed ID: 7834074   DOI: 10.1111/j.1440-1827.1994.tb02920.x

1671  Yamate J, Tajima M, Togo M, Shibuya K, Ihara M, Kudow S  Heterogeneity of Cloned Cell Lines Established from a Transplantable Rat Malignamnt Fibrous Histiocytoma  Jpn J Cancer Res  1991  82:298-307  PubMed ID: 1850724   DOI: 10.1111/j.1349-7006.1991.tb01846.x

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