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Cell No. : Cell Name
RCB0762 : MT-9  update : 2022/03/23
CommentRat cell line derived from malignant fibrous histiocytoma. Possessing characteristics of histiocytes.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yamate, Jyoji
Originator Yamate, Jyoji
Year of deposit 1992
Original cell MT-P
Animal rat < Mammals
Strain name F344/DuCrj
Genus Rattus
Species norvegicus
Gender Male
Age at sampling 15 months
Tissue subcutis of the head
Disease name Malignant Fibrous Histiocytoma
Metastastatic ability No
Classification cancer
Year of origin 1986
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_5338
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 16
User's Publication 0


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Reference
3495  Takashi Kotera 1 , Chisa Katou-Ichikawa, Anusha Hemamali Tennakoon, Miyuu Tanaka, Natsuki Tanaka, Takeshi Izawa, Mitsuru Kuwamura, Jyoji Yamate   Rat malignant fibrous histiocytoma (MFH)-derived cloned cell lines (MT-8 and MT-9) show different differentiation in mesenchymal stem cell lineage  Eep Toxicol Pathol  2015  67(10):499-507  PubMed ID: 26208870   DOI: 10.1016/j.etp.2015.07.004
3493  Ryo Yano, Hossain M Golbar, Takeshi Izawa, Osamu Sawamoto, Mitsuru Kuwamura , Jyoji Yamate  Participation of bone morphogenetic protein (BMP)-6 and osteopontin in cisplatin (CDDP)-induced rat renal fibrosis  Eep Toxicol Pathol  2015  67(2):99-107  PubMed ID: 25467747   DOI: 10.1016/j.etp.2014.10.002
3490  Takeshi Izawa, Takashi Horiuchi, Machi Atarashi, Mitsuru Kuwamura, Jyoji Yamate   Anti-fibrotic Role of miR-214 in Thioacetamide-induced Liver Cirrhosis in Rats  Toxicol Pathol  2015  43(6):844-51  PubMed ID: 25755099   DOI: 10.1177/0192623315573587
3494  Jyoji Yamate, Keiko Ogata, Takahiro Yuasa, Mitsuru Kuwamura, Shigeo Takenaka, Daijirou Kumagai, Kazuyuki Itoh, Jonathan LaMarre  Adipogenic, osteogenic and myofibrogenic differentiations of a rat malignant fibrous histiocytoma (MFH)-derived cell line, and a relationship of MFH cells with embryonal mesenchymal, perivascular and bone marrow stem cells  Eur J Cancer  2007  43(18):2747-56  PubMed ID: 18036812   DOI: 10.1016/j.ejca.2007.10.021
3491  Jyoji Yamate, Masanori Kuribayashi, Mitsuru Kuwamura, Takao Kotani, Kikumi Ogihara  Differential immunoexpressions of cytoskeletons in renal epithelial and interstitial cells in rat and canine fibrotic kidneys, and in kidney-related cell lines under fibrogenic stimuli  Eep Toxicol Pathol   2005  57(2):135-47.  PubMed ID: 16325524   DOI: 10.1016/j.etp.2005.07.001
2786  Tsunenari I, Yamate J, Iwaki M, Kuwamura M, Kotani T, Sakuma S  Angioarchitecture of tumors induced by two different cloned cell lines established from a transplantable rat malignant fibrous histiocytoma.  Microsc Microanal  2003  9:532-41  PubMed ID: 14750988  
2788  Kumagai D, Yamate J, Tajima T, Tsukamoto Y, Yasui H, Kuwamura M, Kotani T, Sakuma S  Distribution of cells labelled by a monoclonal antibody (A3) against a cloned cell line derived from a rat malignant fibrous histiocytoma  J Comp Pathol  2001  123:77-87  PubMed ID: 11032659  
2783  Yamate J, Maeda M, Benn SJ, Laithwaite JE, Allan A, Ide M, Kuwamura M, Kotani T, Sakuma S, Lamarre J  Differential effects of transforming growth factor-beta1, a fibrogenic factor, on macrophage-like cells (HS-P) and myofibroblastic cells (MT-9) in vitro.  Toxicol Pathol  2001  29:483-91  PubMed ID: 11560254  
2784  Yamate J, Maeda M, Benn SJ, Laithwaite JE, Allan A, Ide M, Kuwamura M, Kotani T, Sakuma S, LaMarre J  Effects of lipopolysaccharide on a macrophage-like cell line (HS-P) from a rat histiocytic sarcoma.  J Comp Pathol  2001  125:15-24  PubMed ID: 11437512  
2787  Kumagai D, Yamate J, Tajima T, Kuwamura M, Kotani T, Sakuma S  Malignant fibrous histiocytoma (MFH) cells and macrophages/histiocytes have a common antigen recognized by a monoclonal antibody risen against a rat MFH-derived cloned cell line.  J Vet Med Sci  2001  63:333-5  PubMed ID: 11307938  
2789  Tsunenari I, Yamate J, Sharma KD, Kawachi M, Sakuma S  Expressions of vascular endothelial growth factor and basic fibroblast growth factor in tumors induced by two different cloned cell lines established from transplantable rat malignant fibrous histiocytoma.  J Vet Med Sci  2000  62:699-705  PubMed ID: 10945286  
2792  Yamate J, Tajima M, Shibuya K, Kuwamura M, Kotani T, Sakuma S, Takeya M  Heterogeneity in the origin and immunophenotypes of "histiocytic" cells in transplantable rat malignant fibrous histiocytoma.  J Vet Med Sci.  1996  58:603-609  PubMed ID: 8844594  
2790  Yamate J, Tajima M, Shibuya K, Kuwamura M, Kotani T, Sakuma S  Phenotypic changes in lipopolysaccharide-treated cloned cells derived from transplantable rat malignant fibrous histiocytoma.  J Vet Med Sci  1996  58:1017-20  PubMed ID: 8916004  
2791  Yamate J, Tajima M, Shibuya K, Kuwamura M, Kotani T, Sakuma S  Phenotypic modulation in cisplatin-resistant cloned cells derived from transplantable rat malignant fibrous histiocytoma.  Pathol Int  1996  46:557-67  PubMed ID: 8893224  
2793  Yamate J, Tajima M, Shibuya K, Saitoh T  Enhancement of lipid phagocytosis by cloned cells derived from rat malignant fibrous histiocytoma under hyperlipemic conditions.  Pathol Int  1994  44:735-44  PubMed ID: 7834074  
1671  Yamate J, Tajima M, Togo M, Shibuya K, Ihara M, Kudow S  Heterogeneity of Cloned Cell Lines Established from a Transplantable Rat Malignamnt Fibrous Histiocytoma  Jpn J Cancer Res  1991  82:298-307  PubMed ID: 1850724  

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