Cell No. : Cell Name
RCB0761 : 505-05-01
update : 2022/03/30
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| Comment | Methylcholanthrene-induced fibrosarcoma. Metastatic to lung. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Niwa, Ohtsura
|
| Originator |
Kodama, Yuso
|
| Year of deposit |
1992
|
| Original cell |
505
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C57BCF1
|
| Gender |
Female
|
| Tissue |
back
|
| Disease name |
fibrosarcoma
|
| Metastatic ability |
Yes
|
| Metastatic tissue |
lung
|
| Classification |
cancer
|
| Memo_1 |
Mycoplasma was eliminated with MC-210 by the depositor.
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_E059
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
MEM ALPHA + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
4
|
| User's Publication |
0
|
| Reference |
|
85
Niwa O, Enoki Y, Sadamoto S, Kamiya K, Yokoro K.
Random process of metastasis and generation of heterogeneity in a mouse sarcoma line
Jpn J Cancer Res
1993
84(1):42-7
PubMed ID: 8449827
DOI: 10.1111/j.1349-7006.1993.tb02782.x
|
|
83
Takada T, Suzuki S, Sugawara Y, Kominami R, Arakawa M, Niwa O, Yokoro K.
Somatic mutation during metastasis of a mouse fibrosarcoma line detected by DNA fingerprint analysis
Jpn J Cancer Res
1992
83(2):165-70
PubMed ID: 1555998
DOI: 10.1111/j.1349-7006.1992.tb00082.x
|
|
1787
Enoki Y, Niwa O, Yokoro K, Toge T
Analysis of Clonal Evolution in a Tumor Consisting of pSV2neo-transfected Mouse Fibrosarcoma Clones
Jpn J Cancer Res
1990
81:141-147
PubMed ID: 2110131
DOI: 10.1111/j.1349-7006.1990.tb02540.x
|
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1786
Kodama Y, Seyama T, Kamiya K, Yokoro K
Establishment and immunologic characterization of 3-methylcholanthrene-induced sarcoma cell lines metastasizing widely in mice and exhibiting distinct and selective propensities for the mode of metastasis.
J Natl Cancer Inst
1982
69:595-605
PubMed ID: 7050499
|
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