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Cell No. : Cell Name
RCB0761 : 505-05-01  update : 2022/03/30
CommentMethylcholanthrene-induced fibrosarcoma. Metastatic to lung.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Niwa, Ohtsura
Originator Kodama, Yuso
Year of deposit 1992
Original cell 505
Animal _mouse < Mammals
Strain name C57BCF1
Gender Female
Tissue back
Disease name fibrosarcoma
Metastastatic ability Yes
Metastatsd tissue lung
Classification cancer
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_E059
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 4
User's Publication 0

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85  Niwa O, Enoki Y, Sadamoto S, Kamiya K, Yokoro K.  Random process of metastasis and generation of heterogeneity in a mouse sarcoma line  Jpn J Cancer Res  1993  84(1):42-7  PubMed ID: 8449827   DOI: 10.1111/j.1349-7006.1993.tb02782.x
83  Takada T, Suzuki S, Sugawara Y, Kominami R, Arakawa M, Niwa O, Yokoro K.  Somatic mutation during metastasis of a mouse fibrosarcoma line detected by DNA fingerprint analysis  Jpn J Cancer Res  1992  83(2):165-70  PubMed ID: 1555998   DOI: 10.1111/j.1349-7006.1992.tb00082.x
1787  Enoki Y, Niwa O, Yokoro K, Toge T  Analysis of Clonal Evolution in a Tumor Consisting of pSV2neo-transfected Mouse Fibrosarcoma Clones  Jpn J Cancer Res  1990  81:141-147  PubMed ID: 2110131   DOI: 10.1111/j.1349-7006.1990.tb02540.x
1786  Kodama Y, Seyama T, Kamiya K, Yokoro K  Establishment and immunologic characterization of 3-methylcholanthrene-induced sarcoma cell lines metastasizing widely in mice and exhibiting distinct and selective propensities for the mode of metastasis.  J Natl Cancer Inst  1982  69:595-605  PubMed ID: 7050499  

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