Cell No. : Cell Name
RCB0760 : MT-8
update : 2022/03/23
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Comment | Rat cell line derived from malignant fibrous histiocytoma. Characteristics of histiocytes are lacking. The tumor formed by this cell line consists of immature cells. |
Comment from the depositor | |
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Yamate, Jyoji
|
Originator |
Yamate, Jyoji
|
Year of deposit |
1992
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Original cell |
MT-P
|
Animal |
_rat
< Mammals
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Strain name |
F344/DuCrj
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Genus |
Rattus
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Species |
norvegicus
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Gender |
Male
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Age at sampling |
15 months
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Tissue |
subcutis of the head
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Disease name |
Malignant fibrous histiocytoma
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Metastatic ability |
No
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Classification |
cancer
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Year of origin |
1986
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|
Lifespan |
infinite
|
Morphology |
fibroblast-like
|
Cellosaurus(Expasy) |
CVCL_5337
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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Culture medium |
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DMEM (low glucose) + 10% FBS
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Antibiotics |
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Free
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Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 8 split
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SC frequency |
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Subculture : 2 times/week
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Temperature |
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37
℃
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CO2 concentration |
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5
%
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Freeze medium |
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Medium + 10% DMSO
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Freezing method |
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Slow freezing
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Mycoplasma/Acholeplasma |
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(-)
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Animal PCR |
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OK
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Isozyme |
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LD, NP
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Images |
deposit info | lot info |
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Reference information |
Reference |
13
|
User's Publication |
0
|
Reference |
17473
Nishina H, Katou-Ichikawa C, Kuramochi M, Izawa T, Kuwamura M, Yamate J.
The localization and distribution of cells labeled by a somatic stem cell-recognizing antibody (A3) in rat colon development; possible presence of a new cell type forming the intestinal stem cell niche
J Toxicol Pathol
2019
32(1):37-48
PubMed ID: 30739994
DOI: 10.1293/tox.2018-0037
|
3495
Takashi Kotera 1 , Chisa Katou-Ichikawa, Anusha Hemamali Tennakoon, Miyuu Tanaka, Natsuki Tanaka, Takeshi Izawa, Mitsuru Kuwamura, Jyoji Yamate
Rat malignant fibrous histiocytoma (MFH)-derived cloned cell lines (MT-8 and MT-9) show different differentiation in mesenchymal stem cell lineage
Eep Toxicol Pathol
2015
67(10):499-507
PubMed ID: 26208870
DOI: 10.1016/j.etp.2015.07.004
|
3494
Jyoji Yamate, Keiko Ogata, Takahiro Yuasa, Mitsuru Kuwamura, Shigeo Takenaka, Daijirou Kumagai, Kazuyuki Itoh, Jonathan LaMarre
Adipogenic, osteogenic and myofibrogenic differentiations of a rat malignant fibrous histiocytoma (MFH)-derived cell line, and a relationship of MFH cells with embryonal mesenchymal, perivascular and bone marrow stem cells
Eur J Cancer
2007
43(18):2747-56
PubMed ID: 18036812
DOI: 10.1016/j.ejca.2007.10.021
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3418
J Yamate, Y Yokoyama, D Kumagi, Y Tsukamoto, M Kuwamura, T Kotani, S Sakuma
Tumour lines from a spontaneous rat endometrial stromal sarcoma, showing dendritic cell-like and myofibroblastic cell-like phenotypes
J Comp Pathol
2004
131(1):38-51
PubMed ID: 15144798
DOI: 10.1016/j.jcpa.2004.01.003
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2786
Tsunenari I, Yamate J, Iwaki M, Kuwamura M, Kotani T, Sakuma S
Angioarchitecture of tumors induced by two different cloned cell lines established from a transplantable rat malignant fibrous histiocytoma.
Microsc Microanal
2003
9:532-41
PubMed ID: 14750988
DOI: 10.1017/S1431927603030289
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2788
Kumagai D, Yamate J, Tajima T, Tsukamoto Y, Yasui H, Kuwamura M, Kotani T, Sakuma S
Distribution of cells labelled by a monoclonal antibody (A3) against a cloned cell line derived from a rat malignant fibrous histiocytoma
J Comp Pathol
2001
123:77-87
PubMed ID: 11032659
DOI: 10.1053/jcpa.2000.0389
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2787
Kumagai D, Yamate J, Tajima T, Kuwamura M, Kotani T, Sakuma S
Malignant fibrous histiocytoma (MFH) cells and macrophages/histiocytes have a common antigen recognized by a monoclonal antibody risen against a rat MFH-derived cloned cell line.
J Vet Med Sci
2001
63:333-5
PubMed ID: 11307938
DOI: 10.1292/jvms.63.333
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2789
Tsunenari I, Yamate J, Sharma KD, Kawachi M, Sakuma S
Expressions of vascular endothelial growth factor and basic fibroblast growth factor in tumors induced by two different cloned cell lines established from transplantable rat malignant fibrous histiocytoma.
J Vet Med Sci
2000
62:699-705
PubMed ID: 10945286
DOI: 10.1292/jvms.62.699
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2792
Yamate J, Tajima M, Shibuya K, Kuwamura M, Kotani T, Sakuma S, Takeya M
Heterogeneity in the origin and immunophenotypes of "histiocytic" cells in transplantable rat malignant fibrous histiocytoma.
J Vet Med Sci.
1996
58:603-609
PubMed ID: 8844594
DOI: 10.1292/jvms.58.603
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2790
Yamate J, Tajima M, Shibuya K, Kuwamura M, Kotani T, Sakuma S
Phenotypic changes in lipopolysaccharide-treated cloned cells derived from transplantable rat malignant fibrous histiocytoma.
J Vet Med Sci
1996
58:1017-20
PubMed ID: 8916004
DOI: 10.1292/jvms.58.10_1017
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2791
Yamate J, Tajima M, Shibuya K, Kuwamura M, Kotani T, Sakuma S
Phenotypic modulation in cisplatin-resistant cloned cells derived from transplantable rat malignant fibrous histiocytoma.
Pathol Int
1996
46:557-67
PubMed ID: 8893224
DOI: 10.1111/j.1440-1827.1996.tb03654.x
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2793
Yamate J, Tajima M, Shibuya K, Saitoh T
Enhancement of lipid phagocytosis by cloned cells derived from rat malignant fibrous histiocytoma under hyperlipemic conditions.
Pathol Int
1994
44:735-44
PubMed ID: 7834074
DOI: 10.1111/j.1440-1827.1994.tb02920.x
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1671
Yamate J, Tajima M, Togo M, Shibuya K, Ihara M, Kudow S
Heterogeneity of Cloned Cell Lines Established from a Transplantable Rat Malignamnt Fibrous Histiocytoma
Jpn J Cancer Res
1991
82:298-307
PubMed ID: 1850724
DOI: 10.1111/j.1349-7006.1991.tb01846.x
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