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Cell No. : Cell Name
RCB0752 : TKD2  update : 2022/03/23
CommentMouse kidney cell line. Derived from a transgenic mouse expressing temperature sensitive SV40 large T antigen (SV40tsA58).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Yanai, Nobuyuki
Year of deposit 1992
Animal mouse < Mammals
Gender Unknown
Age at sampling 8 weeks
Tissue kidney
Classification semi-normal
Recombinant recombinant
Exogene SV40 large T (tsA58)
Year of origin 1989
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_5598
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HF-RITC80-7 + 2% FBS + 1μg/ml Insulin + 10μg/ml Transferrin + 10ng/ml mouse EGF
Antibiotics Free
Passage method 25ug/ml pronase
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 1


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Reference
1989  Yanai N, Satoh T, Kyo S, Abe K, Suzuki M, Obinata M  A tubule cell line established from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene.  Jpn J Cancer Res  1991  82:1344-1348  PubMed ID: 1663919

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User's Publication
15851  Shimo T, Adachi Y, Yamanouchi S, Tsuji S, Kimata T, Umezawa K, Okigaki M, Takaya J, Ikehara S, Kaneko K.  A novel nuclear factor κB inhibitor, dehydroxymethylepoxyquinomicin, ameliorates puromycin aminonucleoside-induced nephrosis in mice  Am J Nephrol  2013  37(4):302-9  PubMed ID: 23548793



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