Cell No. : Cell Name RCB0752 : TKD2  update : 2024/08/14
Comment			Mouse kidney cell line. Derived from a transgenic mouse expressing temperature sensitive SV40 large T antigen (SV40tsA58).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Obinata, Masuo
		Originator	Yanai, Nobuyuki
		Year of deposit	1992
		Animal	_mouse < Mammals
		Gender	Unknown
		Age at sampling	8 weeks
		Tissue	kidney
		Classification	semi-normal
		Recombinant	recombinant
		Exogene	SV40 large T (tsA58)
		Year of origin	1989
		Lifespan	infinite
		Morphology	other
		Cellosaurus(Expasy)	CVCL_5598
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		HF-RITC80-7 + 2% FBS + 1μg/ml Insulin + 10μg/ml Transferrin + 10ng/ml mouse EGF
		Antibiotics		Free
		Passage method		25ug/ml pronase
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : 2 times/week
		Temperature		33 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% FBS + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		SSLP(mouse)		OK
		Isozyme		LD, NP
Reference information		Reference	1
		User's Publication	2

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Reference

1989  Yanai N, Satoh T, Kyo S, Abe K, Suzuki M, Obinata M  A tubule cell line established from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene.  Jpn J Cancer Res  1991  82:1344-1348  PubMed ID: 1663919   DOI: 10.1111/j.1349-7006.1991.tb01803.x

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User's Publication

22014  Ueda S, Tominaga T, Ochi A, Sakurai A, Nishimura K, Shibata E, Wakino S, Tamaki M, Nagai K.  TGF-β1 is involved in senescence-related pathways in glomerular endothelial cells via p16 translocation and p21 induction  Sci Rep  2021  11(1):21643  PubMed ID: 34737348   DOI: 10.1038/s41598-021-01150-4

15851  Shimo T, Adachi Y, Yamanouchi S, Tsuji S, Kimata T, Umezawa K, Okigaki M, Takaya J, Ikehara S, Kaneko K.  A novel nuclear factor κB inhibitor, dehydroxymethylepoxyquinomicin, ameliorates puromycin aminonucleoside-induced nephrosis in mice  Am J Nephrol  2013  37(4):302-9  PubMed ID: 23548793   DOI: 10.1159/000348803