Cell No. : Cell Name RCB0750 : TLR2  update : 2022/03/22
Comment			Hepatocytes containing temperature sensitive SV40 large T antigen. P450IA1 inducible.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Obinata, Masuo
		Originator	Yanai, Nobuaki
		Year of deposit	1992
		Animal	_mouse < Mammals
		Gender	Unknown
		Age at sampling	8 weeks
		Tissue	liver
		Disease name	tsSV40 T antigen transgenic
		Classification	semi-normal
		Recombinant	recombinant
		Exogene	SV40 large T (tsA58)
		Year of origin	1989
		Lifespan	infinite
		Morphology	epithelial-like
		Cellosaurus(Expasy)	CVCL_5600
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		HF-RITC80-7 + 2% FBS + 1μg/ml Insulin + 10μg/ml Transferrin + 10ng/ml mouse EGF
		Antibiotics		Free
		Passage method		0.25% Trypsin
		Coating dish		type I collagen coated dish
	Culture information	Passage ratio		1 : 3 split
		SC frequency		Subculture : 2 times/week
		Temperature		33 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% FBS + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		SSLP(mouse)		OK
		Isozyme		LD, NP
Reference information		Reference	2
		User's Publication	4

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Reference

73  Yanai N, Obinata M.  Apoptosis is induced at nonpermissive temperature by a transient increase in p53 in cell lines immortalized with temperature-sensitive SV40 large T-antigen gene  Exp Cell Res  1994  211(2):296-300  PubMed ID: 8143776   DOI: 10.1006/excr.1994.1090

1990  Yanai N, Suzuki M, Obinata M  Hepatocyte cell lines established from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene.  Exp Cell Res  1991  197:50-6  PubMed ID: 1655502   DOI: 10.1016/0014-4827(91)90478-d

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User's Publication

15799  Chen J, Wei Y, He J, Cui G, Zhu Y, Lu C, Ding Y, Xue R, Bai L, Uede T, Li L, Diao H.  Natural killer T cells play a necessary role in modulating of immune-mediated liver injury by gut microbiota  Sci Rep  2014  4:7259  PubMed ID: 25435303   DOI: 10.1038/srep07259

15717  Baba A, Ohtake F, Okuno Y, Yokota K, Okada M, Imai Y, Ni M, Meyer CA, Igarashi K, Kanno J, Brown M, Kato S.  PKA-dependent regulation of the histone lysine demethylase complex PHF2-ARID5B  Nat Cell Biol  2011  13(6):668-75  PubMed ID: 21532585   DOI: 10.1038/ncb2228

18423  Diao H, Iwabuchi K, Li L, Onoe K, Van Kaer L, Kon S, Saito Y, Morimoto J, Denhardt DT, Rittling S, Uede T.  Osteopontin regulates development and function of invariant natural killer T cells  Proc Natl Acad Sci U S A  2008  105(41):15884-9  PubMed ID: 18836077   DOI: 10.1073/pnas.0806089105

19144  Matsumoto G, Tsunematsu S, Tsukinoki K, Ohmi Y, Iwamiya M, Oliveira-dos-Santos A, Tone D, Shindo J, Penninger JM.  Essential role of the adhesion receptor LFA-1 for T cell-dependent fulminant hepatitis  J Immunol  2002  169(12):7087-96  PubMed ID: 12471145   DOI: 10.4049/jimmunol.169.12.7087