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Cell No. : Cell Name
RCB0750 : TLR2  update : 2022/03/22
CommentHepatocytes containing temperature sensitive SV40 large T antigen. P450IA1 inducible.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Obinata, Masuo
Originator Yanai, Nobuaki
Year of deposit 1992
Animal _mouse < Mammals
Gender Unknown
Age at sampling 8 weeks
Tissue liver
Disease name tsSV40 T antigen transgenic
Classification semi-normal
Recombinant recombinant
Exogene SV40 large T (tsA58)
Year of origin 1989
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_5600
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HF-RITC80-7 + 2% FBS + 1μg/ml Insulin + 10μg/ml Transferrin + 10ng/ml mouse EGF
Antibiotics Free
Passage method 0.25% Trypsin
Coating dish type I collagen coated dish
Culture information Passage ratio 1 : 3 split
SC frequency Subculture : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 2
User's Publication 3

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73  Yanai N, Obinata M.  Apoptosis is induced at nonpermissive temperature by a transient increase in p53 in cell lines immortalized with temperature-sensitive SV40 large T-antigen gene  Exp Cell Res  1994  211(2):296-300  PubMed ID: 8143776   DOI: 10.1006/excr.1994.1090
1990  Yanai N, Suzuki M, Obinata M  Hepatocyte cell lines established from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene.  Exp Cell Res  1991  197:50-6  PubMed ID: 1655502   DOI: 10.1016/0014-4827(91)90478-d

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User's Publication
15799  Chen J, Wei Y, He J, Cui G, Zhu Y, Lu C, Ding Y, Xue R, Bai L, Uede T, Li L, Diao H.  Natural killer T cells play a necessary role in modulating of immune-mediated liver injury by gut microbiota  Sci Rep  2014  4:7259  PubMed ID: 25435303   DOI: 10.1038/srep07259
15717  Baba A, Ohtake F, Okuno Y, Yokota K, Okada M, Imai Y, Ni M, Meyer CA, Igarashi K, Kanno J, Brown M, Kato S.  PKA-dependent regulation of the histone lysine demethylase complex PHF2-ARID5B  Nat Cell Biol  2011  13(6):668-75  PubMed ID: 21532585   DOI: 10.1038/ncb2228
18423  Diao H, Iwabuchi K, Li L, Onoe K, Van Kaer L, Kon S, Saito Y, Morimoto J, Denhardt DT, Rittling S, Uede T.  Osteopontin regulates development and function of invariant natural killer T cells  Proc Natl Acad Sci U S A  2008  105(41):15884-9  PubMed ID: 18836077   DOI: 10.1073/pnas.0806089105

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