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Cell No. : Cell Name
RCB0749 : MFH-ino  update : 2023/11/30
CommentHuman fibrous histiocytoma. Showing fibroblastic- and histiocytic features.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
Social Insurance Funabashi Central Hospital

6-13-10 Kaijin, Funabashi, Chiba 273-8556 Japan
Fax. +81-47-437-9545
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Takahashi, Kikuo
Originator Takahashi, Kikuo
Year of deposit 1992
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Tissue maxilla
Disease name malignant fibrous histiocytoma
Classification cancer
Year of origin 1987
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_1407
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 60-129(50) : /62(5),63(7),64(6),65(20),66(40),67(2)/
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 2

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1583  Takahashi K, Sato K  Establishment and characterization of a human neoplastic cell line (MFH-ino) derived from malignant fibrous histiocytoma of maxilla.  Hum Cell  1991  4:51-7  PubMed ID: 1653597  

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User's Publication
4428  Yamashita H, Osaki M, Ardyanto TD, Osaki M, Yoshida H, Ito H.  Cyclooxygenase-2 in human malignant fibrous histiocytoma: correlations with intratumoral microvessel density, expression of vascular endothelial growth factor and thymidine phosphorylase  Int J Mol Med  2004  14(4):565-70  PubMed ID: 15375582  
6475  Osaki M, Osaki M, Yamashita H, Shomori K, Yoshida H, Ito H.  Expression of minichromosome maintenance-2 in human malignant fibrous histiocytomas: Correlations with Ki-67 and P53 expression, and apoptosis.  Int J Mol Med  2002  10(2):161-8  PubMed ID: 12119552  

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