Cell No. : Cell Name
RCB0748 : Mm1
update : 2024/02/13
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| Comment | Spontaneouly differentiated cell line derived from M1. Non-tumorigenic. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Takagi, Kuniaki
|
| Originator |
Maeda, Michiyuki
|
| Year of deposit |
1992
|
| Another name |
Mouse macrophage like cell
|
| Original cell |
M1
|
| Animal |
_mouse
< Mammals
|
| Strain name |
SL
|
| Tissue |
blood
|
| Disease name |
leukemia
|
| Classification |
cancer
|
|
Lifespan |
infinite
|
| Morphology |
other
|
| Cellosaurus(Expasy) |
CVCL_5557
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| |
deposit info |
lot info |
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Medium |
Medium List |
|
Culture type |
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Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 5% HS (GIBCO)
|
| Antibiotics |
|
Free
|
|
Passage method |
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1mM EDTA in PBS(-), washed twice, then seeded
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Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
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37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
4
|
| User's Publication |
2
|
| Reference |
|
1532
Takagi K, Hosaka T, Suketa Y
Effect of recombinant human interleukin-6 on nitrite production of mouse myeloid leukemia cells.
J Cell Physiol
1991
147:306-10
PubMed ID: 2040662
DOI: 10.1002/jcp.1041470216
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1533
Hirai, K I, Nagata K, Maeda M, Ichikawa Y
Changes in ultrastructures and enzyme activities during differentiation of myeloid leukemia cells to normal macrophages.
Exp Cell Res
1979
124:269-83
PubMed ID: 228952
DOI: 10.1016/0014-4827(79)90203-9
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72
Yodoi J, Masuda T, Miyama M, Maeda M, Ichikawa Y.
Interaction of lymphocytes and macrophage cell line cells (M1 cells). I. Functional maturation and appearance of Fc receptors im M1 cells
Cell Immunol
1978
39(1):5-17
PubMed ID: 568034
DOI: 10.1016/0008-8749(78)90077-1
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579
Maeda M, Ichikawa Y
Spontaneous development of macrophage-like cells in a culture of myeloid leukemia cells.
Gann
1973
64:265-71
PubMed ID: 4517230
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