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Cell No. : Cell Name
RCB0748 : Mm1  update : 2021/01/08
CommentSpontaneouly differentiated cell line derived from M1. Non-tumorigenic.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Takagi, Kuniaki
Originator Maeda, Michiyuki
Year of deposit 1992
Another name Mouse macrophage like cell
Original cell M1
Animal mouse < Mammals
Strain name SL
Tissue blood
Case history leukemia
Classification cancer
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 5% HS
Antibiotics Free
Passage method 1mM EDTA in PBS(-), washed twice, then seeded
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 3
User's Publication 2


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Reference
1532  Takagi K, Hosaka T, Suketa Y  Effect of recombinant human interleukin-6 on nitrite production of mouse myeloid leukemia cells.  J Cell Physiol  1991  147:306-10  PubMed ID: 2040662
1533  Hirai, K I, Nagata K, Maeda M, Ichikawa Y  Changes in ultrastructures and enzyme activities during differentiation of myeloid leukemia cells to normal macrophages.  Exp Cell Res  1979  124:269-83  PubMed ID: 228952
579  Maeda M, Ichikawa Y  Spontaneous development of macrophage-like cells in a culture of myeloid leukemia cells.  Gann  1973  64:265-71  PubMed ID: 4517230

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User's Publication
10916  Yuko Fujihara, Tsuyoshi Takato, Kazuto Hoshi  Immunological Response to Tissue-Engineered Cartilage Derived From Auricular Chondrocytes and a PLLA Scaffold in Transgenic Mice  Biomaterials  2010  31(6):1227-34  PubMed ID: 19913296
3347  Nishio, Koji, Qiao, Shanlou, Yamashita, Hitoshi  Characterization of the differential expression of uncoupling protein 2 and ROS production in differentiated mouse macrophage-cells (Mm1) and the progenitor cells (M1).  J Mol Histol  2005  36:35-44  PubMed ID: 15703997



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