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Cell No. : Cell Name
RCB0738 : MTHC-1  update : 2022/03/22
CommentRat thymoma cell line without mouse chromosome contamination. Compare to MTHC-2 and -3.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Matsuyama, Mutsushi
Originator Hiai, H. & Matsuyama, M.
Year of deposit 1992
Animal rat < Mammals
Strain name BUF/Mna
Genus Rattus
Species norvegicus
Gender Female
Age at sampling 18 months
Tissue thymus
Disease name thymoma
Classification cancer
Year of origin 1987
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_8733
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, MPI, PepB
Reference information Reference 1
User's Publication 0


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Reference
1891  Lu J, Sakai Y, Wajjawalku W, Isobe K, Saito M, Amo H, Kojima A, Utsumi, K R, Takahashi M, Hiai H, et al.  Establishment of Transplantable Tumor Lines and in vitro Cell Lines of Malignant Thymoma Developed in a BUF/Mna Rat  Jpn J Cancer Res  1992  83:618-624  PubMed ID: 1644664  

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