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Cell No. : Cell Name
RCB0736 : DJM-1  update : 2024/10/23
CommentMalignant trichilemmal cyst cells. Strongly laminin positive on cell surface. Serum-free culturable.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Katayama, Hiroshi
Originator Yaoita, Hideo
Year of deposit 1992
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 87 years
Tissue skin
Disease name malignant trichilemmal cyst
Metastatic ability Yes
Metastatic tissue lympho node
Classification cancer
Year of origin 1984
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_1172
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 39-89(50) : /41(2),42(11),43(16),44(10),45(5),81(1)/
STR(human) OK
Images
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lot info
Reference information Reference 16
User's Publication 5


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Reference
939  Suzuki M, Murata S, Yaoita H, Nakagawa H.  An antibody to BP 180 kDa antigen is able to induce an increase of intracellular Ca2+ concentration in DJM-1 (human squamous cell carcinoma) cells  Autoimmunity  2002  35(4):271-6  PubMed ID: 12482196  
68  Suzuki M, Harada S, Yaoita H, Nakagawa H.  Bullous pemphigoid IgG2 and IgG4 antibodies have a capability to inhibit a transient increase of intracellular Ca2+ induced by bullous pemphigoid IgG1 antibody  Autoimmunity  1999  30(2):85-91  PubMed ID: 10435721   DOI: 10.3109/08916939908994765
62  Yamane Y, Sato H, Higashi K, Yaoita H.  Linear immunoglobulin A (IgA) bullous dermatosis of childhood: identification of the target antigens and study of the cellular sources  Br J Dermatol  1996  135(5):785-90  PubMed ID: 8977683  
63  uzuki M, Harada S, Owaribe K, Yaoita H.  Intracellular ionic changes induced by bullous pemphigoid IgG subclasses  Autoimmunity  1996  23(3):181-97  PubMed ID: 8879454   DOI: 10.3109/08916939608995342
59  Kitajima Y, Owada MK, Fujisawa Y, Seishima M, Yaoita H, Hirako Y, Owaribe K.  A hemidesmosomal transmembrane collagenous molecule, the 180-kDa bullous pemphigoid antigen (BPA II), is phosphorylated with 12-O-tetradecanoylphorbol-13-acetate in a human squamous cell carcinoma cell line (DJM-1)  Epithelial Cell Biol  1995  4(2):70-5  PubMed ID: 8688920  
65  Kitajima Y, Hirako Y, Owaribe K, Yaoita H.  A possible cell-biologic mechanism involved in blister formation of bullous pemphigoid: anti-180-kD BPA antibody is an initiator  Dermatology  1994  189 Suppl 1:46-9  PubMed ID: 8049562   DOI: 10.1159/000246928
66  Kitajima Y, Hirako Y, Owaribe K, Mori S, Yaoita H.  Antibody-binding to the 180-kD bullous pemphigoid antigens at the lateral cell surface causes their internalization and inhibits their assembly at the basal cell surface in cultured keratinocytes  J Dermatol  1994  21(11):838-4  PubMed ID: 7852645   DOI: 10.1111/j.1346-8138.1994.tb03299.x
69  Suzuki M, Harada S, Kanazawa K, Kitajima Y, Yaoita H.  Relationship between antigens and IgG subclasses in bullous pemphigoid  Autoimmunity  1994  18(3):217-25  PubMed ID: 7858107   DOI: 10.3109/08916939409007999
60  Kitajima Y, Owaribe K, Nishizawa Y, Jokura Y, Yaoita H.  Phorbol ester- and calcium-induced reorganization of 180-kDa bullous pemphigoid antigen on the ventral surface of cultured human keratinocytes as studied by immunofluorescence and immunoelectron microscopy  Exp Cell Res  1992  203(1):17-24  PubMed ID: 1426040   DOI: 10.1016/0014-4827(92)90034-6
67  Kitajima Y, Owaribe K, Nishizawa Y, Yaoita H.  Control of the distribution of hemidesmosome components in cultured keratinocytes: Ca2+ and phorbol esters  J Dermatol  1992  19(11):770-3  PubMed ID: 1284069   DOI: 10.1111/j.1346-8138.1992.tb03778.x
1575  Katayama H, Kitagawa S, Masuyama J, Yaoita H  Polymorphonuclear leukocyte-induced detachment of cultured epidermal carcinoma cells from the substratum.  J Invest Dermatol  1991  97:949-52  PubMed ID: 1919059   DOI: 10.1111/1523-1747.ep12491818
61  Sheu HM, Kitajima Y, Yaoita H.  Involvement of protein kinase C in translocation of desmoplakins from cytosol to plasma membrane during desmosome formation in human squamous cell carcinoma cells grown in low to normal calcium concentration  Exp Cell Res  1989  185(1):176-90  PubMed ID: 2509226   DOI: 10.1016/0014-4827(89)90047-5
64  Nagao S, Kitajima Y, Nagata K, Inoue S, Yaoita H, Nozawa Y.  Correlation between cell-cell contact formation and activation of protein kinase C in a human squamous cell carcinoma cell line  J Invest Dermatol  1989  92(2):175-8  PubMed ID: 2465349   DOI: 10.1111/1523-1747.ep12276697
71  Yamane Y, Kitajima Y, Yaoita H.  Characterization of bullous pemphigoid antigen synthesized by cultured human squamous cell carcinoma cells  J Invest Dermatol  1989  93(2):220-3  PubMed ID: 2666514   DOI: 10.1111/1523-1747.ep12277575
1573  Kitajima Y, Inoue S, Nagao S, Nagata K, Yaoita H, Nozawa Y  Biphasic effects of 12-O-tetradecanoylphorbol-13-acetate on the cell morphology of low calcium-grown human epidermal carcinoma cells: involvement of translocation and down regulation of protein kinase C.  Cancer Res  1988  48:964-70  PubMed ID: 2448028  
1574  Kitajima Y, Inoue S, Yaoita H  Effects of pemphigus antibody on the regeneration of cell-cell contact in keratinocyte cultures grown in low to normal Ca++ concentration.  J Invest Dermatol  1987  89:167-71  PubMed ID: 2439611   DOI: 10.1111/1523-1747.ep12470554

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User's Publication
6021  Hasegawa G, Akatsuka K, Nakashima Y, Yokoe Y, Higo N, Shimonaka M.  Tamoxifen inhibits the proliferation of non‑melanoma skin cancer cells by increasing intracellular calcium concentration.  Int J Oncol  2018  53(5):2157-2166  PubMed ID: 30226592   DOI: 10.3892/ijo.2018.4548
6030  Nobeyama Y, Umezawa Y, Nakagawa H.  Silencing of homeobox A5 gene in the stratum corneum of psoriasis.  Exp Dermatol  2017  26(11):1068-1074  PubMed ID: 28482119   DOI: 10.1111/exd.13377
14745  Egashira S, Jinnin M, Ajino M, Shimozono N, Okamoto S, Tasaki Y, Hirano A, Ide M, Kajihara I, Aoi J, Harada M, Igata T, Masuguchi S, Fukushima S, Ihn H.  Chronic sun exposure-related fusion oncogenes EGFR-PPARGC1A in cutaneous squamous cell carcinoma  Sci Rep  2017  7(1):12654  PubMed ID: 28978917   DOI: 10.1038/s41598-017-12836-z
9885  Nobeyama Y, Nakagawa H.  Aberrant DNA Methylation in Keratoacanthoma.  PLoS ONE  2016  11:e0165370  PubMed ID: 27788211   DOI: 10.1371/journal.pone.0165370
2632  Takeuchi, Tamotsu, Liang, Sheng-Ben, Matsuyoshi, Norihisa, Zhou, Shuxia, Miyachi, Yoshiki, Sonobe, Hiroshi, Ohtsuki, Yuji  Loss of T-cadherin (CDH13, H-cadherin) expression in cutaneous squamous cell carcinoma.  Lab Invest  2002  82:1023-9  PubMed ID: 12177241   DOI: 10.1097/01.lab.0000025391.35798.f1


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