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Cell No. : Cell Name
RCB0736 : DJM-1  update : 2022/07/07
CommentMalignant trichilemmal cyst cells. Strongly laminin positive on cell surface. Serum-free culturable.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Katayama, Hiroshi
Originator Yaoita, Hideo
Year of deposit 1992
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 87 years
Tissue skin
Disease name malignant trichilemmal cyst
Metastastatic ability Yes
Metastatsd tissue lympho node
Classification cancer
Year of origin 1984
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_1172
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 39-89(50) : /41(2),42(11),43(16),44(10),45(5),81(1)/
STR(human) OK
deposit info
lot info
Reference information Reference 3
User's Publication 5

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1575  Katayama H, Kitagawa S, Masuyama J, Yaoita H  Polymorphonuclear leukocyte-induced detachment of cultured epidermal carcinoma cells from the substratum.  J Invest Dermatol  1991  97:949-52  PubMed ID: 1919059   DOI: 10.1111/1523-1747.ep12491818
1573  Kitajima Y, Inoue S, Nagao S, Nagata K, Yaoita H, Nozawa Y  Biphasic effects of 12-O-tetradecanoylphorbol-13-acetate on the cell morphology of low calcium-grown human epidermal carcinoma cells: involvement of translocation and down regulation of protein kinase C.  Cancer Res  1988  48:964-70  PubMed ID: 2448028  
1574  Kitajima Y, Inoue S, Yaoita H  Effects of pemphigus antibody on the regeneration of cell-cell contact in keratinocyte cultures grown in low to normal Ca++ concentration.  J Invest Dermatol  1987  89:167-71  PubMed ID: 2439611   DOI: 10.1111/1523-1747.ep12470554

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User's Publication
6021  Hasegawa G, Akatsuka K, Nakashima Y, Yokoe Y, Higo N, Shimonaka M.  Tamoxifen inhibits the proliferation of non‑melanoma skin cancer cells by increasing intracellular calcium concentration.  Int J Oncol  2018  53(5):2157-2166  PubMed ID: 30226592   DOI: 10.3892/ijo.2018.4548
6030  Nobeyama Y, Umezawa Y, Nakagawa H.  Silencing of homeobox A5 gene in the stratum corneum of psoriasis.  Exp Dermatol  2017  26(11):1068-1074  PubMed ID: 28482119   DOI: 10.1111/exd.13377
14745  Egashira S, Jinnin M, Ajino M, Shimozono N, Okamoto S, Tasaki Y, Hirano A, Ide M, Kajihara I, Aoi J, Harada M, Igata T, Masuguchi S, Fukushima S, Ihn H.  Chronic sun exposure-related fusion oncogenes EGFR-PPARGC1A in cutaneous squamous cell carcinoma  Sci Rep  2017  7(1):12654  PubMed ID: 28978917   DOI: 10.1038/s41598-017-12836-z
9885  Nobeyama Y, Nakagawa H.  Aberrant DNA Methylation in Keratoacanthoma.  PLoS ONE  2016  11:e0165370  PubMed ID: 27788211   DOI: 10.1371/journal.pone.0165370
2632  Takeuchi, Tamotsu, Liang, Sheng-Ben, Matsuyoshi, Norihisa, Zhou, Shuxia, Miyachi, Yoshiki, Sonobe, Hiroshi, Ohtsuki, Yuji  Loss of T-cadherin (CDH13, H-cadherin) expression in cutaneous squamous cell carcinoma.  Lab Invest  2002  82:1023-9  PubMed ID: 12177241   DOI: 10.1097/01.lab.0000025391.35798.f1

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