Cell No. : Cell Name
RCB0736 : DJM-1
update : 2024/10/23
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| Comment | Malignant trichilemmal cyst cells. Strongly laminin positive on cell surface. Serum-free culturable. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Katayama, Hiroshi
|
| Originator |
Yaoita, Hideo
|
| Year of deposit |
1992
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Female
|
| Age at sampling |
87 years
|
| Tissue |
skin
|
| Disease name |
malignant trichilemmal cyst
|
| Metastatic ability |
Yes
|
| Metastatic tissue |
lympho node
|
| Classification |
cancer
|
| Year of origin |
1984
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|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_1172
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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MEM + 10% FBS
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| Antibiotics |
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Free
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|
Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : once/week, Medium Renewal : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Virus (HIV) |
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(-)
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| Isozyme |
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LD, NP
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| Chromosome mode |
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39-89(50) : /41(2),42(11),43(16),44(10),45(5),81(1)/
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| STR(human) |
|
OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
16
|
| User's Publication |
5
|
| Reference |
|
939
Suzuki M, Murata S, Yaoita H, Nakagawa H.
An antibody to BP 180 kDa antigen is able to induce an increase of intracellular Ca2+ concentration in DJM-1 (human squamous cell carcinoma) cells
Autoimmunity
2002
35(4):271-6
PubMed ID: 12482196
|
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68
Suzuki M, Harada S, Yaoita H, Nakagawa H.
Bullous pemphigoid IgG2 and IgG4 antibodies have a capability to inhibit a transient increase of intracellular Ca2+ induced by bullous pemphigoid IgG1 antibody
Autoimmunity
1999
30(2):85-91
PubMed ID: 10435721
DOI: 10.3109/08916939908994765
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62
Yamane Y, Sato H, Higashi K, Yaoita H.
Linear immunoglobulin A (IgA) bullous dermatosis of childhood: identification of the target antigens and study of the cellular sources
Br J Dermatol
1996
135(5):785-90
PubMed ID: 8977683
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63
uzuki M, Harada S, Owaribe K, Yaoita H.
Intracellular ionic changes induced by bullous pemphigoid IgG subclasses
Autoimmunity
1996
23(3):181-97
PubMed ID: 8879454
DOI: 10.3109/08916939608995342
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59
Kitajima Y, Owada MK, Fujisawa Y, Seishima M, Yaoita H, Hirako Y, Owaribe K.
A hemidesmosomal transmembrane collagenous molecule, the 180-kDa bullous pemphigoid antigen (BPA II), is phosphorylated with 12-O-tetradecanoylphorbol-13-acetate in a human squamous cell carcinoma cell line (DJM-1)
Epithelial Cell Biol
1995
4(2):70-5
PubMed ID: 8688920
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65
Kitajima Y, Hirako Y, Owaribe K, Yaoita H.
A possible cell-biologic mechanism involved in blister formation of bullous pemphigoid: anti-180-kD BPA antibody is an initiator
Dermatology
1994
189 Suppl 1:46-9
PubMed ID: 8049562
DOI: 10.1159/000246928
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66
Kitajima Y, Hirako Y, Owaribe K, Mori S, Yaoita H.
Antibody-binding to the 180-kD bullous pemphigoid antigens at the lateral cell surface causes their internalization and inhibits their assembly at the basal cell surface in cultured keratinocytes
J Dermatol
1994
21(11):838-4
PubMed ID: 7852645
DOI: 10.1111/j.1346-8138.1994.tb03299.x
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69
Suzuki M, Harada S, Kanazawa K, Kitajima Y, Yaoita H.
Relationship between antigens and IgG subclasses in bullous pemphigoid
Autoimmunity
1994
18(3):217-25
PubMed ID: 7858107
DOI: 10.3109/08916939409007999
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60
Kitajima Y, Owaribe K, Nishizawa Y, Jokura Y, Yaoita H.
Phorbol ester- and calcium-induced reorganization of 180-kDa bullous pemphigoid antigen on the ventral surface of cultured human keratinocytes as studied by immunofluorescence and immunoelectron microscopy
Exp Cell Res
1992
203(1):17-24
PubMed ID: 1426040
DOI: 10.1016/0014-4827(92)90034-6
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67
Kitajima Y, Owaribe K, Nishizawa Y, Yaoita H.
Control of the distribution of hemidesmosome components in cultured keratinocytes: Ca2+ and phorbol esters
J Dermatol
1992
19(11):770-3
PubMed ID: 1284069
DOI: 10.1111/j.1346-8138.1992.tb03778.x
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1575
Katayama H, Kitagawa S, Masuyama J, Yaoita H
Polymorphonuclear leukocyte-induced detachment of cultured epidermal carcinoma cells from the substratum.
J Invest Dermatol
1991
97:949-52
PubMed ID: 1919059
DOI: 10.1111/1523-1747.ep12491818
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61
Sheu HM, Kitajima Y, Yaoita H.
Involvement of protein kinase C in translocation of desmoplakins from cytosol to plasma membrane during desmosome formation in human squamous cell carcinoma cells grown in low to normal calcium concentration
Exp Cell Res
1989
185(1):176-90
PubMed ID: 2509226
DOI: 10.1016/0014-4827(89)90047-5
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64
Nagao S, Kitajima Y, Nagata K, Inoue S, Yaoita H, Nozawa Y.
Correlation between cell-cell contact formation and activation of protein kinase C in a human squamous cell carcinoma cell line
J Invest Dermatol
1989
92(2):175-8
PubMed ID: 2465349
DOI: 10.1111/1523-1747.ep12276697
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71
Yamane Y, Kitajima Y, Yaoita H.
Characterization of bullous pemphigoid antigen synthesized by cultured human squamous cell carcinoma cells
J Invest Dermatol
1989
93(2):220-3
PubMed ID: 2666514
DOI: 10.1111/1523-1747.ep12277575
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1573
Kitajima Y, Inoue S, Nagao S, Nagata K, Yaoita H, Nozawa Y
Biphasic effects of 12-O-tetradecanoylphorbol-13-acetate on the cell morphology of low calcium-grown human epidermal carcinoma cells: involvement of translocation and down regulation of protein kinase C.
Cancer Res
1988
48:964-70
PubMed ID: 2448028
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1574
Kitajima Y, Inoue S, Yaoita H
Effects of pemphigus antibody on the regeneration of cell-cell contact in keratinocyte cultures grown in low to normal Ca++ concentration.
J Invest Dermatol
1987
89:167-71
PubMed ID: 2439611
DOI: 10.1111/1523-1747.ep12470554
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| User's Publication |
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6021
Hasegawa G, Akatsuka K, Nakashima Y, Yokoe Y, Higo N, Shimonaka M.
Tamoxifen inhibits the proliferation of non‑melanoma skin cancer cells by increasing intracellular calcium concentration.
Int J Oncol
2018
53(5):2157-2166
PubMed ID: 30226592
DOI: 10.3892/ijo.2018.4548
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6030
Nobeyama Y, Umezawa Y, Nakagawa H.
Silencing of homeobox A5 gene in the stratum corneum of psoriasis.
Exp Dermatol
2017
26(11):1068-1074
PubMed ID: 28482119
DOI: 10.1111/exd.13377
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14745
Egashira S, Jinnin M, Ajino M, Shimozono N, Okamoto S, Tasaki Y, Hirano A, Ide M, Kajihara I, Aoi J, Harada M, Igata T, Masuguchi S, Fukushima S, Ihn H.
Chronic sun exposure-related fusion oncogenes EGFR-PPARGC1A in cutaneous squamous cell carcinoma
Sci Rep
2017
7(1):12654
PubMed ID: 28978917
DOI: 10.1038/s41598-017-12836-z
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9885
Nobeyama Y, Nakagawa H.
Aberrant DNA Methylation in Keratoacanthoma.
PLoS ONE
2016
11:e0165370
PubMed ID: 27788211
DOI: 10.1371/journal.pone.0165370
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2632
Takeuchi, Tamotsu, Liang, Sheng-Ben, Matsuyoshi, Norihisa, Zhou, Shuxia, Miyachi, Yoshiki, Sonobe, Hiroshi, Ohtsuki, Yuji
Loss of T-cadherin (CDH13, H-cadherin) expression in cutaneous squamous cell carcinoma.
Lab Invest
2002
82:1023-9
PubMed ID: 12177241
DOI: 10.1097/01.lab.0000025391.35798.f1
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