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Cell No. : Cell Name
RCB0721 : GOTO·P3  update : 2022/03/22
CommentSubline of GOTO. Protein-free medium adapted.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Takaoka, Toshiko
Originator Sekiguchi, M.
Year of deposit 1992
Original cell GOTO
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 1 year
Tissue neuroblastoma
Classification cancer
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_2911
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DM-201
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 2-3 split
SC frequency Subculture : once/1-2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium DM-201 : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
STR(human) OK
Reference information Reference 10
User's Publication 2

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1651  Sugimoto T, Kato T, Sawada T, Horii Y, Kemshead, J T, Hino T, Morioka H, Hosoi H  Schwannian cell differentiation of human neuroblastoma cell lines in vitro induced by bromodeoxyuridine.  Cancer Res  1988  48:2531-7  PubMed ID: 2833349
1662  Tsuji S, Yamashita T, Nagai Y  A novel, carbohydrate signal-mediated cell surface protein phosphorylation: ganglioside GQ1b stimulates ecto-protein kinase activity on the cell surface of a human neuroblastoma cell line, GOTO.  J Biochem (Tokyo)  1988  104:498-503  PubMed ID: 3240992
1650  Tsunamoto K, Todo S, Imashuku S, Kato K  Induction of S 100 protein by 5-bromo-2'-deoxyuridine in human neuroblastoma cell lines.  Cancer Res  1988  48:170-4  PubMed ID: 3334991
1653  Imashuku, S., Todo, S., Esumi, N., Hasida, T., Shimizu, Y., Fujiwara, F., Tsunamoto, K., Nakajima, F.    Proc Am Assoc Cancer  1986  27:274 
1656  Fujiwara F, Todo S, Imashuku S  Antitumor effect of gamma-linolenic acid on cultured human neuroblastoma cells.  Prostaglandins Leukot Med  1986  23:311-20  PubMed ID: 3020590
1657  Sohmura Y, Nakata K, Yoshida H, Kashimoto S, Matsui Y, Furuichi H  Recombinant human tumor necrosis factor--II. Antitumor effect on murine and human tumors transplanted in mice.  Int J Immunopharmacol  1986  8:357-68  PubMed ID: 3525434
1661  Nakajima J, Tsuji S, Nagai Y  Bioactive gangliosides: analysis of functional structures of the tetrasialoganglioside GQ1b which promotes neurite outgrowth.  Biochim Biophys Acta  1986  876:65-71  PubMed ID: 3947670
1649  Tsuji S, Arita M, Nagai Y  GQ1b, a bioactive ganglioside that exhibits novel nerve growth factor (NGF)-like activities in the two neuroblastoma cell lines.  J Biochem (Tokyo)  1983  94:303-306  PubMed ID: 6619115
128  Sekiguchi M, Oota T, Sakakibara K, Inui N, Fujii G  Establishment and characterization of a human neuroblastoma cell line in tissue culture.  Jpn J Exp Med  1979  49:67-83  PubMed ID: 470188
1648  Ishikawa S  Differentiation of human neuroblastoma cells in vitro--morphological changes induced by dibutyrl cyclic AMP.  Acta Pathol Jpn  1977  27:697-711  PubMed ID: 201151

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User's Publication
6145  Wang HQ, Imai Y, Kataoka A, Takahashi R.  Cell type-specific upregulation of Parkin in response to ER stress.  Antioxid Redox Signal  2007  9(5):533-42  PubMed ID: 17465879
13711  Zhang L, Yeger H, Das B, Irwin MS, Baruchel S.  Tissue microenvironment modulates CXCR4 expression and tumor metastasis in neuroblastoma  Neoplasia  2007  9(1):36-46  PubMed ID: 17325742

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