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Cell No. : Cell Name
RCB0711 : HyGPD·YK-1-1  update : 2022/03/22
CommentAnti-rabbit muscle glyceraldehyde-3-phosphate dehydrogenase mAb (IgG). Serum-free culturable.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Shirahata, Sanetaka
Originator Kichise, Yoshikatsu
Year of deposit 1991
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_L551
hybridoma Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name P3-X63-Ag8.653
Fusion_cell_2 strain mouse
Fusion method PEG, HAT selection
Antibody name HyGPD・YK-1-1
Subclass IgG
Immunogen Rabbit muscle G3PDHase (Boehringer-Mannheim)
Antigen_name Rabbit muscle GPD
Antigen_m.w. 14.4kDa
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives E-RDF + 5% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 3 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG2b
Chromosome mode 45-96(50) : /84(3),85(4),86(11),87(2),88(3),89(3)/
Reference information Reference 0
User's Publication 0


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