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Cell No. : Cell Name
RCB0708 : HF10B4  update : 2022/03/22
CommentAnti-human lung carcinoma mAb (IgM). Possible to culture in serum-free medium.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Shirahata, Sanetaka
Originator Murakami, Hiroki
Year of deposit 1991
Animal human x human < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_C473
hybridoma Fusion_cell_1 name lymphocyte
Fusion_cell_1 strain human
Fusion_cell_2 name NAT-30
Fusion_cell_2 strain human
Fusion method PEG, HAT selection
Antibody name HF10B4
Subclass IgM
Immunogen human lymphocytes from lymph nodes of lung carcino
Antigen_name human lung carcinoma
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives E-RDF + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 3 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgM
Chromosome mode 46-49(50) : 46(1),47(14),48(31),49(4)
Reference information Reference 1
User's Publication 1


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Reference
1460  Murakami H, Hashizume S, Ohashi H, Shinohara K, Yasumoto K, Nomoto K, Omura H  Human-human hybridomas secreting antibodies specific to human lung carcinoma.  In Vitro Cell Dev Biol  1985  21:593-6  PubMed ID: 3902783  

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User's Publication
3571  Mikami S, Kobayashi T, Yokoyama S, Imataka H.  A hybridoma-based in vitro translation system that efficiently synthesizes glycoproteins.  J Biotechnol  2006  127(1):65-78  PubMed ID: 16889861   DOI: 10.1016/j.jbiotec.2006.06.018



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