細胞番号 : 細胞名
RCB0705 : 10H
update : 2024/01/17
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細胞特性(Comment:英) | Anti poly(ADP-ribose) IgG3 kappa producing. |
細胞特性(日) | 抗ADP-ribose IgG3 kappaを産生するハイブリドーマ。 |
細胞特性(寄託者記述:英) | |
細胞特性(寄託者記述:日) | |
使用条件(英) | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biochemistry.1984;23:3771-3777) designated by the DEPOSITOR is required. |
使用条件(日) | 研究成果の発表にあたり寄託者の指定する文献 (Biochemistry 1984;23:3771-3777) を引用すること。 |
備考(英) | |
備考(日) | |
提供申込書類(英) |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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提供申込書類(日) |
依頼書C-0001.pdf
 
同意書(非営利学術目的)C-0003.pdf
 
同意書(営利目的)C-0003p.pdf
 
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
細胞基本情報
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寄託者 |
Miwa, Masanao
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樹立者 |
Miwa, Masanao
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寄託日 |
1991
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別名 |
H10A12
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動物種 |
_mouse x mouse
< Hybrid
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採取組織 |
hybridoma
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細胞分類 |
hybrid cells
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細胞寿命 |
infinite
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細胞形態 |
lymphocyte-like
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Cellosaurus(Expasy) |
CVCL_N538
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ハイブリドーマ関連
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親細胞1の細胞名 |
spleen
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親細胞1の動物名及び系統名 |
mouse, BALB/c
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親細胞2の細胞名 |
P3・NS-1/1・Ag4.1
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親細胞2の動物名及び系統名 |
mouse
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細胞融合・選択法 |
Kohler & Milstein method
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単クローン性抗体名 |
anti poly ADP ribose
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サブクラス |
IgG3
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免疫に用いた材料 |
poly(ADP-rib)
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抗体で検出される抗原の名称 |
poly ADP ridose
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分子量 |
10000〜200000
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抗体の認識部位 |
poly ADP ribose
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Suspension cells
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培養方法 |
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General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
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培地 |
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DMEM (low glucose) + 10% FBS
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抗生物質 |
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Free
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継代方法 |
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dilution
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継代密度 |
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1 : 10 split
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継代・培地交換頻度 |
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Subculture : 2 times/week
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培養最適温度 |
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37
℃
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二酸化炭素濃度 |
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5
%
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凍結培地 |
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Medium + 10% DMSO
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凍結方法 |
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Slow freezing
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マイコプラズマ/アコレプラズマ |
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(-)
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産生抗体のサブクラス |
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IgG3
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文献情報 |
Reference(英) |
9件
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Reference(日) |
0件
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利用者成果(英) |
6件
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利用者成果(日) |
0件
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Reference(英) |
20973
Ida C, Yamashita S, Eguchi T, Kuroda Y, Nakae S, Nishi Y, Kamemura K, Shirai T, Mizukami T, Tanaka M, Moss J, Miwa M.
An Enzyme-Linked Immunosorbent Assay to Quantify Poly (ADP-Ribose) Level In Vivo.
Methods Mol Biol
2022
2609:91-100
PubMed ID: 36515831
DOI: 10.1007/978-1-0716-2891-1_6
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17105
Masanao Miwa, Chieri Ida, Sachiko Yamashita, Kenichi Kouyama, Yasuhito Kuroda, Takayuki Eguchi, Narumi Ohta, Teruaki Sato, Masataka Tsuda, Masakazu Tanaka
In Vivo Level of Poly(ADP-ribose)
Challenges
2018
9:23
DOI: 10.3390/challe9010023
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857
Ida C, Yamashita S, Tsukada M, Sato T, Eguchi T, Tanaka M, Ogata S, Fujii T, Nishi Y, Ikegami S, Moss J, Miwa M.
An enzyme-linked immunosorbent assay-based system for determining the physiological level of poly(ADP-ribose) in cultured cells
Anal Biochem
2016
494:76-81
PubMed ID: 26548958
DOI: 10.1016/j.ab.2015.10.014
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855
Shitara Y, Tonohora Y, Goto T, Yamada Y, Miki T, Makino H, Miwa M, Komiya T.
Mitochondrial P5, a member of protein disulphide isomerase family, suppresses oxidative stress-induced cell death
J Biochem
2012
152(1):73-85
PubMed ID: 22492663
DOI: 10.1093/jb/mvs034
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936
Hanai S, Kanai M, Ohashi S, Okamoto K, Yamada M, Takahashi H, Miwa M.
Loss of poly(ADP-ribose) glycohydrolase causes progressive neurodegeneration in Drosophila melanogaster
Proc Natl Acad Sci U S A
2004
101(1):82-6
PubMed ID: 14676324
DOI: 10.1073/pnas.2237114100
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856
Kanai M, Tong WM, Sugihara E, Wang ZQ, Fukasawa K, Miwa M.
Involvement of poly(ADP-Ribose) polymerase 1 and poly(ADP-Ribosyl)ation in regulation of centrosome function
Mol Cell Biol
2003
23(7):2451-62
PubMed ID: 12640128
DOI: 10.1128/MCB.23.7.2451-2462.2003
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2839
Masutani, Mitsuko, Miwa, Masanao
Poly(ADP-ribose) polymerase and cancer: in relation to the lectures presented by Dr Gilbert de Murcia.
Jpn J Clin Oncol
2002
32:483-7
PubMed ID: 12499423
DOI: 10.1093/jjco/hyf101
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2840
Soldani C, Lazze, M C, Bottone, M G, Tognon G, Biggiogera M, Pellicciari, C E, Scovassi, A I
Poly(ADP-ribose) polymerase cleavage during apoptosis: when and where?
Exp Cell Res
2001
269:193-201
PubMed ID: 11570811
DOI: 10.1006/excr.2001.5293
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1788
Kawamitsu H, Hoshino H, Okada H, Miwa M, Momoi H, Sugimura T
Monoclonal antibodies to poly(adenosine diphosphate ribose) recognize different structures.
Biochemistry
1984
23:3771-7
PubMed ID: 6206890
DOI: 10.1021/bi00311a032
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利用者成果(英) |
20824
Blessing C, Apelt K, van den Heuvel D, Gonzalez-Leal C, Rother MB, van der Woude M, González-Prieto R, Yifrach A, Parnas A, Shah RG, Kuo TT, Boer DEC, Cai J, Kragten A, Kim HS, Schärer OD, Vertegaal ACO, Shah GM, Adar S, Lans H, van Attikum H, Ladurner AG, Luijsterburg MS.
XPC-PARP complexes engage the chromatin remodeler ALC1 to catalyze global genome DNA damage repair.
Nat Commun
2022
13(1):4762
PubMed ID: 35963869
DOI: 10.1038/s41467-022-31820-4
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14171
Purohit NK, Robu M, Shah RG, Geacintov NE, Shah GM.
Characterization of the interactions of PARP-1 with UV-damaged DNA in vivo and in vitro
Sci Rep
2016
6:19020
PubMed ID: 26753915
DOI: 10.1038/srep19020
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18686
Ghodgaonkar MM, Zacal N, Kassam S, Rainbow AJ, Shah GM.
Depletion of poly(ADP-ribose) polymerase-1 reduces host cell reactivation of a UV-damaged adenovirus-encoded reporter gene in human dermal fibroblasts
DNA Repair (Amst)
2008
7(4):617-32
PubMed ID: 18289944
DOI: 10.1016/j.dnarep.2008.01.001
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19051
Geraets L, Moonen HJ, Brauers K, Wouters EF, Bast A, Hageman GJ.
Dietary flavones and flavonoles are inhibitors of poly(ADP-ribose)polymerase-1 in pulmonary epithelial cells
J Nutr
2007
137(10):2190-5
PubMed ID: 17884996
DOI: 10.1093/jn/137.10.2190
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19050
Geraets L, Moonen HJ, Wouters EF, Bast A, Hageman GJ.
Caffeine metabolites are inhibitors of the nuclear enzyme poly(ADP-ribose)polymerase-1 at physiological concentrations
Biochem Pharmacol
2006
72(7):902-10
PubMed ID: 16870158
DOI: 10.1016/j.bcp.2006.06.023
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2886
Ju BG, Solum D, Song EJ, Lee KJ, Rose DW, Glass CK, Rosenfeld MG
Activating the PARP-1 sensor component of the groucho/ TLE1 corepressor complex mediates a CaMKinase IIdelta-dependent neurogenic gene activation pathway.
Cell
2004
119:815-29
PubMed ID: 15607978
DOI: 10.1016/j.cell.2004.11.017
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