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Cell No. : Cell Name
RCB0705 : 10H  update : 2022/03/22
CommentAnti poly(ADP-ribose) IgG3 kappa producing.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biochemistry.1984;23:3771-3777) designated by the DEPOSITOR is required.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Miwa, Masanao
Originator Miwa, Masanao
Year of deposit 1991
Another name H10A12
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_N538
hybridoma Fusion_cell_1 name spleen
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name P3・NS-1/1・Ag4.1
Fusion_cell_2 strain mouse
Fusion method Kohler & Milstein method
Antibody name anti poly ADP ribose
Subclass IgG3
Immunogen poly(ADP-rib)
Antigen_name poly ADP ridose
Antigen_m.w. 10000〜200000
Antigen_epitope poly ADP ribose
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG3
Reference information Reference 4
User's Publication 3

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17105  Masanao Miwa, Chieri Ida, Sachiko Yamashita, Kenichi Kouyama, Yasuhito Kuroda, Takayuki Eguchi, Narumi Ohta, Teruaki Sato, Masataka Tsuda, Masakazu Tanaka  In Vivo Level of Poly(ADP-ribose)  Challenges  2018  9:23 
2839  Masutani, Mitsuko, Miwa, Masanao  Poly(ADP-ribose) polymerase and cancer: in relation to the lectures presented by Dr Gilbert de Murcia.  Jpn J Clin Oncol  2002  32:483-7  PubMed ID: 12499423
2840  Soldani C, Lazze, M C, Bottone, M G, Tognon G, Biggiogera M, Pellicciari, C E, Scovassi, A I  Poly(ADP-ribose) polymerase cleavage during apoptosis: when and where?  Exp Cell Res  2001  269:193-201  PubMed ID: 11570811
1788  Kawamitsu H, Hoshino H, Okada H, Miwa M, Momoi H, Sugimura T  Monoclonal antibodies to poly(adenosine diphosphate ribose) recognize different structures.  Biochemistry  1984  23:3771-7  PubMed ID: 6206890

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User's Publication
20824  Blessing C, Apelt K, van den Heuvel D, Gonzalez-Leal C, Rother MB, van der Woude M, González-Prieto R, Yifrach A, Parnas A, Shah RG, Kuo TT, Boer DEC, Cai J, Kragten A, Kim HS, Schärer OD, Vertegaal ACO, Shah GM, Adar S, Lans H, van Attikum H, Ladurner AG, Luijsterburg MS.  XPC-PARP complexes engage the chromatin remodeler ALC1 to catalyze global genome DNA damage repair.  Nat Commun  2022  13(1):4762  PubMed ID: 35963869
14171  Purohit NK, Robu M, Shah RG, Geacintov NE, Shah GM.  Characterization of the interactions of PARP-1 with UV-damaged DNA in vivo and in vitro  Sci Rep  2016  6:19020  PubMed ID: 26753915
2886  Ju BG, Solum D, Song EJ, Lee KJ, Rose DW, Glass CK, Rosenfeld MG  Activating the PARP-1 sensor component of the groucho/ TLE1 corepressor complex mediates a CaMKinase IIdelta-dependent neurogenic gene activation pathway.  Cell  2004  119:815-29  PubMed ID: 15607978

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