細胞番号 : 細胞名
RCB0704 : WI-38
update : 2022/07/13
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| 細胞特性(Comment:英) | The most famous normal human fibroblast. Obtained directly from L. Hayflick. |
| 細胞特性(日) | 著名なヒト正常２倍体線維芽細胞。樹立者であるHayflickより直接入手したもの。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | No restriction, but the user needs depositor's approval to use for any commercial purpose. |
| 使用条件(日) | 特に制限を設けない。ただし、商業目的で利用する場合は、寄託者の承諾を得ること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Hayflick, Leonard
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| 樹立者 |
Hayflick, Leonard
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| 寄託日 |
1991
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| 動物種 |
human
< Mammals
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| 属名 |
Homo
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| 種名 |
sapiens
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| 性別 |
Female
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| 齢 |
3 months gestation
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| 採取組織 |
embryo/fetus, lung
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| 細胞分類 |
normal
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| 入手歴 |
overseas
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細胞寿命 |
finite
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| 細胞形態 |
fibroblast-like
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| Cellosaurus(Expasy) |
CVCL_0579
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent cells
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| 培地 |
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MEM + 10% FBS
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| 抗生物質 |
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Free
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継代方法 |
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0.25% Trypsin
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継代密度 |
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1 : 4 split
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| 継代・培地交換頻度 |
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Subculture : 1-2 times/week, Medium Renewal : 2 times/week
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| 培養最適温度 |
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37
℃
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| 二酸化炭素濃度 |
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5
%
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| 凍結培地 |
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Medium + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ |
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(-)
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| ウィルス（HIV) |
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(-)
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| アイソザイム検査 |
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LD, NP
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| 染色体検査 |
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45-92(50) : 45(4),46(44),92(2)
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| 個体識別検査 |
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OK
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| 画像情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
20件
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| Reference(日) |
1件
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| 利用者成果(英) |
16件
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| 利用者成果(日) |
0件
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| Reference(英) |
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4807
Olshansky SJ1, Hayflick L2.
The Role of the WI-38 Cell Strain in Saving Lives and Reducing Morbidity.
AIMS Public Health
2017
4(2):127-138
PubMed ID: 29546209
DOI: 10.3934/publichealth.2017.2.127
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4808
Meredith Wadman
Medical research: cell division
Nature
2013
2013-06-27
PubMed ID: 23803825
DOI: 10.1038/498422a
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4806
Hayflick L.
Paying for tissue: the case of WI-38.
Science
2012
37(6100):1292
PubMed ID: 22984048
DOI: 10.1126/science.337.6100.1292-a
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4805
Hayflick L.
A novel technique for transforming the theft of mortal human cells into praiseworthy federal policy.
Exp Gerontol
1998
33(1-2):191-207
PubMed ID: 9467730
DOI: 10.1016/s0531-5565(97)00115-0
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4804
Hayflick L.
Antecedents of cell aging research.
Exp Gerontol
1989
24(5-6):355-65
PubMed ID: 2698813
DOI: 10.1016/0531-5565(89)90043-0
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4803
Hayflick L.
The cell biology of aging.
Clin Geriatr Med
1985
1(1):15-27
PubMed ID: 3913498
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4802
Matsumura T, Pfendt EA, Zerrudo Z, Hayflick L.
Senescent human diploid cells (WI-38). Attempted induction of proliferation by infection with SV40 and by fusion with irradiated continuous cell lines.
Exp Cell Res
1980
125(2):453-7
PubMed ID: 6243571
DOI: 10.1016/0014-4827(80)90139-1
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4800
Matsumura T, Pfendt EA, Hayflick L.
DNA synthesis in the human diploid cell strain WI-38 during in vitro aging: an autoradiography study.
J Gerontol
1979
34(3):323-7
PubMed ID: 429764
DOI: 10.1093/geronj/34.3.323
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4801
Matsumura T, Zerrudo Z, Hayflick L.
Senescent human diploid cells in culture: survival, DNA synthesis and morphology.
J Gerontol
1979
34(3):328-34
PubMed ID: 429765
DOI: 10.1093/geronj/34.3.328
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4799
Razin S, Pfendt EA, Matsumura T, Hayflick L.
Comparison by autoradiography of macromolecular biosynthesis in "young" and "old" human diploid fibroblast cultures. A brief note.
Mech Ageing Dev
1977
6(5):379-84
PubMed ID: 895211
DOI: 10.1016/0047-6374(77)90039-2
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4797
Masover GK, Namba M, Hayflick L.
Cytotoxic effect of a T-strain mycoplasma (Ureaplasma urealyticum) on cultured normal human cells (wi-38).
Exp Cell Res
1976
99(2):363-74
PubMed ID: 1269532
DOI: 10.1016/0014-4827(76)90594-2
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4798
Bradley MO, Hayflick L, Schimke RT.
Protein degradation in human fibroblasts (WI-38). Effects of aging, viral transformation, and amino acid analogs.
J Biol Chem
1976
251(12):3521-9
PubMed ID: 931997
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4796
Wright WE, Hayflick L.
The regulation of cellular aging by nuclear events in cultured normal human fibroblasts (WI-38).
Adv Exp Med Biol
1975
61:39-55
PubMed ID: 1180169
DOI: 10.1007/978-1-4615-9032-3_3
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4794
Tomkins GA, Stanbridge EJ, Hayflick L.
Viral probes of aging in the human diploid cell strain WI-38.
Proc Soc Exp Biol Med
1974
146(2):385-90
PubMed ID: 4365308
DOI: 10.3181/00379727-146-38110
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4795
Smith JR, Hayflick L.
Variation in the life-span of clones derived from human diploid cell strains.
J Cell Biol
1974
62(1):48-53
PubMed ID: 4407044
DOI: 10.1083/jcb.62.1.48
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4793
Wright WE, Hayflick L.
Formation of anucleate and multinucleate cells in normal and SV 40 transformed WI-38 by cytochalasin B.
Exp Cell Res
1972
74(1):187-94
PubMed ID: 4342183
DOI: 10.1016/0014-4827(72)90496-x
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4792
Stanbridge E, Onen M, Perkins FT, Hayflick L.
Karyological and morphological characteristics of human diploid cell strain WI-38 infected with mycoplasmas.
Exp Cell Res
1969
57(2):397-410
PubMed ID: 5347639
DOI: 10.1016/0014-4827(69)90166-9
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375
HAYFLICK L.
THE LIMITED IN VITRO LIFETIME OF HUMAN DIPLOID CELL STRAINS.
Exp Cell Res
1965
37:614-36
PubMed ID: 14315085
DOI: 10.1016/0014-4827(65)90211-9
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454
HAYFLICK L, PLOTKIN SA, NORTON TW, KOPROWSKI H.
Preparation of poliovirus vaccines in a human fetal diploid cell strain.
Am J Hyg
1962
75:240-58
PubMed ID: 13905660
DOI: 10.1093/oxfordjournals.aje.a120247
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453
HAYFLICK L, MOORHEAD PS.
The serial cultivation of human diploid cell strains.
Exp Cell Res
1961
25:585-621
PubMed ID: 13905658
DOI: 10.1016/0014-4827(61)90192-6
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| 利用者成果(英) |
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20040
Nara T, Nakagawa Y, Tsuganezawa K, Yuki H, Sekimata K, Koyama H, Ogawa N, Honma T, Shirouzu M, Fukami T, Matsuo Y, Inaoka DK, Kita K, Tanaka A.
The ubiquinone synthesis pathway is a promising drug target for Chagas disease.
PLoS One
2021
16(2):e0243855
PubMed ID: 33539347
DOI: 10.1371/journal.pone.0243855
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20390
Fujita M, Sasada M, Eguchi M, Iyoda T, Okuyama S, Osawa T, Tsuzuranuki K, Sakamoto M, Hagihara Y, Matsumura M, Osada S, Kodama H, Higami Y, Fukai F.
Induction of cellular senescence in fibroblasts through β1-integrin activation by tenascin-C-derived peptide and its protumor effect.
Am J Cancer Res
2021
11(9):4364-4379
PubMed ID: 34659892
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17822
Fujita M, Ito-Fujita Y, Iyoda T, Sasada M, Okada Y, Ishibashi K, Osawa T, Kodama H, Fukai F, Suzuki H.
Peptide TNIIIA2 Derived from Tenascin-C Contributes to Malignant Progression in Colitis-Associated Colorectal Cancer via β1-Integrin Activation in Fibroblasts
Int J Mol Sci
2019
20(11):2752.
PubMed ID: 31195598
DOI: 10.3390/ijms20112752
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4372
Ito T, Rakainsa SK, Nisa K, Morita H.
Three new abietane-type diterpenoids from the leaves of Indonesian Plectranthus scutellarioides.
Fitoterapia
2018
127:146-150
PubMed ID: 29447983
DOI: 10.1016/j.fitote.2018.02.013
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16603
Yuki T, Maniwa Y, Doi T, Okada K, Nishio W, Hayashi Y, Okita Y.
DNA damage sensor protein hRad9, a novel molecular target for lung cancer treatment
Oncol Rep
2008
20(5):1047-52
PubMed ID: 18949400
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3565
Takahashi K, Yoshida N, Murakami N, Kawata K, Ishizaki H, Tanaka-Okamoto M, Miyoshi J, Zinn AR, Shime H, Inoue N.
Dynamic regulation of p53 subnuclear localization and senescence by MORC3.
Mol Biol Cell
2007
18(5):1701-9
PubMed ID: 17332504
DOI: 10.1091/mbc.e06-08-0747
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13731
Takata M, Maniwa Y, Doi T, Tanaka Y, Okada K, Nishio W, Ohbayashi C, Yoshimura M, Hayashi Y, Okita Y.
Double-layered collagen gel hemisphere for cell invasion assay: successful visualization and quantification of cell invasion activity
Cell Commun Adhes
2007
14(4):157-67
PubMed ID: 17957531
DOI: 10.1080/15419060701557859
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8940
Hoshino K, Momiyama E, Yoshida K, Nishimura K, Sakai S, Toida T, Kashiwagi K, Igarashi K.
Polyamine transport by mammalian cells and mitochondria: role of antizyme and glycosaminoglycans.
J. Biol. Chem.
2005
280:42801-8
PubMed ID: 16263714
DOI: 10.1074/jbc.M505445200
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3228
Wu CA, Tsujita M, Hayashi M, Yokoyama S.
Probucol inactivates ABCA1 in the plasma membrane with respect to its mediation of apolipoprotein binding and high density lipoprotein assembly and to its proteolytic degradation.
J Biol Chem
2004
279(29):30168-74
PubMed ID: 15140889
DOI: 10.1074/jbc.M403765200
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3231
Arakawa R, Hayashi M, Remaley AT, Brewer BH, Yamauchi Y, Yokoyama S.
Phosphorylation and stabilization of ATP binding cassette transporter A1 by synthetic amphiphilic helical peptides.
J Biol Chem
2004
279(8):6217-20
PubMed ID: 14701850
DOI: 10.1074/jbc.C300553200
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2978
Takayama Y, Takahashi H, Mizumachi K, Takezawa T.
Low density lipoprotein receptor-related protein (LRP) is required for lactoferrin-enhanced collagen gel contractile activity of human fibroblasts.
J Biol Chem
2003
278(24):22112-8
PubMed ID: 12672816
DOI: 10.1074/jbc.M300894200
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3227
Yamauchi Y, Hayashi M, Abe-Dohmae S, Yokoyama S.
Apolipoprotein A-I activates protein kinase C alpha signaling to phosphorylate and stabilize ATP binding cassette transporter A1 for the high density lipoprotein assembly.
J Biol Chem
2003
278(48):47890-7
PubMed ID: 12952980
DOI: 10.1074/jbc.M306258200
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2977
Takayama Y, Mizumachi K, Takezawa T.
The bovine lactoferrin region responsible for promoting the collagen gel contractile activity of human fibroblasts.
Biochem Biophys Res Commun
2002
299(5):813-7
PubMed ID: 12470651
DOI: 10.1016/s0006-291x(02)02748-1
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2976
Takayama Y, Mizumachi K.
Effects of lactoferrin on collagen gel contractile activity and myosin light chain phosphorylation in human fibroblasts.
FEBS Lett
2001
508(1):111-6
PubMed ID: 11707279
DOI: 10.1016/s0014-5793(01)03041-1
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6665
Takayama Y, Kitsunai K, Mizumachi K.
Factors in bovine colostrum that enhance the migration of human fibroblasts in type I collagen gels.
Biosci Biotechnol Biochem
2001
65(12):2776-9
PubMed ID: 11826977
DOI: 10.1271/bbb.65.2776
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2075
Takano M, Kondo J, Yayama K, Okamoto H.
Expression of low-molecular-weight kininogen mRNA in human fibroblast WI38 cells.
Jpn J Pharmacol
1996
71(4):341-3
PubMed ID: 8886933
DOI: 10.1254/jjp.71.341
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