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Cell No. : Cell Name
RCB0701 : 143B/TK^(-)neo^(R)  update : 2021/05/20
Commentv-Ki-ras transformant TE85 cell line. HAT sensitive, neo resistant.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Sugimoto, Yoshikazu
Originator Croce, C.
Year of deposit 1991
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Unknown
Tissue osteosarcoma
Classification transformed
Recombinant recombinant
Exogene v-Ki-ras
History Dr. Perira-Smith M. Olivia
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_9W36
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 46-125(50) : /48(11),49(13),50(10),51(4),53(2)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 12


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Reference
1531  Pereira-Smith, O M, Smith, J R  Genetic analysis of indefinite division in human cells: identification of four complementation groups.  Proc Natl Acad Sci U S A  1988  85:6042-6  PubMed ID: 3413074

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User's Publication
11888  Yoshida K, Okamoto M, Sasaki J, Kuroda C, Ishida H, Ueda K, Ideta H, Kamanaka T, Sobajima A, Takizawa T, Tanaka M, Aoki K, Uemura T, Kato H, Haniu H, Saito N.  Anti-PD-1 antibody decreases tumour-infiltrating regulatory T cells.  BMC Cancer  2020  20(1):25.  PubMed ID: 31914969
11888  Yoshida K, Okamoto M, Sasaki J, Kuroda C, Ishida H, Ueda K, Ideta H, Kamanaka T, Sobajima A, Takizawa T, Tanaka M, Aoki K, Uemura T, Kato H, Haniu H, Saito N.  Anti-PD-1 antibody decreases tumour-infiltrating regulatory T cells.  BMC Cancer  2020  20(1):25.  PubMed ID: 31914969
13141  Oka N, Komuro A, Amano H, Dash S, Honda M, Ota K, Nishimura S, Ueda T, Akagi M, Okada H.  Ascorbate sensitizes human osteosarcoma cells to the cytostatic effects of cisplatin.  Pharmacol Res Perspect  2020    PubMed ID: 32725721
13306  Ando T, Ichikawa J, Fujimaki T, Taniguchi N, Takayama Y, Haro H.  Gemcitabine and Rapamycin Exhibit Additive Effect against Osteosarcoma by Targeting Autophagy and Apoptosis.  Cancers (Basel)  2020    PubMed ID: 33114161
11978  Asano N, Takeshima H, Yamashita S, Takamatsu H, Hattori N, Kubo T, Yoshida A, Kobayashi E, Nakayama R, Matsumoto M, Nakamura M, Ichikawa H, Kawai A, Kondo T, Ushijima T.  Epigenetic reprogramming underlies efficacy of DNA demethylation therapy in osteosarcomas.  Sci Rep  2019    PubMed ID: 31889115
9465  Tomohiko Tokunaga, Takashi Ando, Miki Suzuki-Karasaki, Tomohisa Ito, Asuka Onoe-Takahashi, Toyoko Ochiai, Masayoshi Soma , Yoshihiro Suzuki-Karasaki  Plasma-stimulated Medium Kills TRAIL-resistant Human Malignant Cells by Promoting Caspase-Independent Cell Death via Membrane Potential and Calcium Dynamics Modulation  Int J Oncol  2018  52(3):697-708  PubMed ID: 29393427
14766  Wada KI, Hosokawa K, Ito Y, Maeda M.  Quantitative control of mitochondria transfer between live single cells using a microfluidic device  Biol Open  2017  6(12):1960-1965  PubMed ID: 29092814
10919    Antitumor activities of BIBF 1120, BI 860585, and BI 836845 in preclinical models of sarcoma  Integr Mol Med  2016  3: 755-760 
8566  Morioka K, Tanikawa C, Ochi K, Daigo Y, Katagiri T, Kawano H, Kawaguchi H, Myoui A, Yoshikawa H, Naka N, Araki N, Kudawara I, Ieguchi M, Nakamura K, Nakamura Y, Matsuda K.  Orphan receptor tyrosine kinase ROR2 as a potential therapeutic target for osteosarcoma.  Cancer Sci.  2009  100:1227-33  PubMed ID: 19486338
9072  M Saijo, T Suzutani, K Mizuta, I Kurane, S Morikawa  Characterization and Susceptibility to Antiviral Agents of Herpes Simplex Virus Type 1 Containing a Unique Thymidine Kinase Gene With an Amber Codon Between the First and the Second Initiation Codons  Arch Virol  2008  153 (2), 303-14  PubMed ID: 18066636
9041  Tatsuo Suzutani, Ken Ishioka, Erik De Clercq, Kei Ishibashi, Hisatoshi Kaneko, Toshihiko Kira, Koh-Ichi Hashimoto, Masahiro Ogasawara, Katsuki Ohtani, Nobutaka Wakamiya, Masayuki Saijo  Differential Mutation Patterns in Thymidine Kinase and DNA Polymerase Genes of Herpes Simplex Virus Type 1 Clones Passaged in the Presence of Acyclovir or Penciclovir  Antimicrob Agents Chemother  2003  47 (5), 1707-13  PubMed ID: 12709344
9034  Masayuki Saijo, Tatsuo Suzutani, Erik De Clercq, Masahiro Niikura, Akihiko Maeda, Shigeru Morikawa, Ichiro Kurane  Genotypic and Phenotypic Characterization of the Thymidine Kinase of ACV-resistant HSV-1 Derived From an Acyclovir-Sensitive Herpes Simplex Virus Type 1 Strain  Antiviral Res  2002  56 (3), 253-62  PubMed ID: 12406508



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