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Cell No. : Cell Name
RCB0698 : HFSKF-II  update : 2022/11/16
CommentHuman fibroblasts derived from fetal skin (11 weekly age).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, Isamu & Ishikawa, Hiroshi
Year of deposit 1991
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Unknown
Age at sampling 11 weeks embryo
Tissue embryo/fetus, skin
Classification normal
Year of origin 1991
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_3709
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF12 + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 45-46(50) : 45(2),46(48)
STR(human) OK
deposit info
lot info
Reference information Reference 0
User's Publication 6

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User's Publication
19960  Nakano M, Sakamoto T, Kitano Y, Bono H, Simpson RJ, Tabunoki H.  An extract from the frass of swallowtail butterfly (Papilio machaon) larvae inhibits HCT116 colon cancer cell proliferation but not other cancer cell types  BMC Genomics  2023  24(1):735  PubMed ID: 38049715   DOI: 10.1186/s12864-023-09841-0
9810  Junko Shibato, Fumiko Takenoya, Takahiro Hirabayashi, Ai Kimura, Yusuke Iwasaki, Yoko Toyoda, Motohide Hori, Shigeru Tamogami, Randeep Rakwal, Seiji Shioda  Towards Identification of Bioactive Compounds in Cold Vacuum Extracted Double Cherry Blossom (Gosen-Sakura) Leaves  Plant Signal Behav  2019  14(10):e1644594  PubMed ID: 31342859   DOI: 10.1080/15592324.2019.1644594
7022  Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M.  CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation.  Nat. Methods  2017  14:963-966  PubMed ID: 28892089   DOI: 10.1038/nmeth.4430
4090  A Sugitachi, K Otsuka, T Kimura, M Hakozaki, M Yaegashi, M Kamishima, K Kume, Y Ohmori, S Nishizuka, A Sasaki  Color-imaging histodiagnostic approach for cancer.  Integrative Molecular Medicine  2015  2:231-233    DOI: 10.15761/IMM.1000145
2860  Nagaoka Y, Otsu K, Okada F, Sato K, Ohba Y, Kotani N, Fujii J  Specific inactivation of cysteine protease-type cathepsin by singlet oxygen generated from naphthalene endoperoxides.  Biochem Biophys Res Commun  2005  331:215-23  PubMed ID: 15845381   DOI: 10.1016/j.bbrc.2005.03.146
9611  Y Hagihara, Y Saitoh, H Iwata, T Taki, S Hirano, N Arita, T Hayakawa  Transplantation of Xenogeneic Cells Secreting Beta-Endorphin for Pain Treatment: Analysis of the Ability of Components of Complement to Penetrate Through Polymer Capsules  Cell Transplant   1997  6(5):527-30  PubMed ID: 9331506   DOI: 10.1177/096368979700600515

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