Cell No. : Cell Name
RCB0698 : HFSKF-II
update : 2024/05/30
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Comment | Human fibroblasts derived from fetal skin (11 weekly age). |
Comment from the depositor | |
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Ishiwata, Isamu
|
Originator |
Ishiwata, Isamu & Ishikawa, Hiroshi
|
Year of deposit |
1991
|
Animal |
_human
< Mammals
|
Genus |
Homo
|
Species |
sapiens
|
Race |
Japanese
|
Gender |
Unknown
|
Age at sampling |
11 weeks embryo
|
Tissue |
embryo/fetus, skin
|
Classification |
normal
|
Year of origin |
1991
|
|
Lifespan |
finite
|
Morphology |
fibroblast-like
|
Cellosaurus(Expasy) |
CVCL_3709
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
Culture medium |
|
HamF12 + 15% FBS
|
Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
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1 : 4 split
|
SC frequency |
|
Subculture : 2 times/week
|
Temperature |
|
37
℃
|
CO2 concentration |
|
5
%
|
Freeze medium |
|
Medium + 10% DMSO
|
Freezing method |
|
Slow freezing
|
Mycoplasma |
|
(-)
|
Virus (HIV) |
|
(-)
|
Isozyme |
|
LD, NP
|
Chromosome mode |
|
45-46(50) : 45(2),46(48)
|
STR(human) |
|
OK
|
Images |
deposit info | lot info |
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Reference information |
Reference |
0
|
User's Publication |
6
|
User's Publication |
19960
Nakano M, Sakamoto T, Kitano Y, Bono H, Simpson RJ, Tabunoki H.
An extract from the frass of swallowtail butterfly (Papilio machaon) larvae inhibits HCT116 colon cancer cell proliferation but not other cancer cell types
BMC Genomics
2023
24(1):735
PubMed ID: 38049715
DOI: 10.1186/s12864-023-09841-0
|
9810
Junko Shibato, Fumiko Takenoya, Takahiro Hirabayashi, Ai Kimura, Yusuke Iwasaki, Yoko Toyoda, Motohide Hori, Shigeru Tamogami, Randeep Rakwal, Seiji Shioda
Towards Identification of Bioactive Compounds in Cold Vacuum Extracted Double Cherry Blossom (Gosen-Sakura) Leaves
Plant Signal Behav
2019
14(10):e1644594
PubMed ID: 31342859
DOI: 10.1080/15592324.2019.1644594
|
7022
Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M.
CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation.
Nat. Methods
2017
14:963-966
PubMed ID: 28892089
DOI: 10.1038/nmeth.4430
|
4090
A Sugitachi, K Otsuka, T Kimura, M Hakozaki, M Yaegashi, M Kamishima, K Kume, Y Ohmori, S Nishizuka, A Sasaki
Color-imaging histodiagnostic approach for cancer.
Integrative Molecular Medicine
2015
2:231-233
DOI: 10.15761/IMM.1000145
|
2860
Nagaoka Y, Otsu K, Okada F, Sato K, Ohba Y, Kotani N, Fujii J
Specific inactivation of cysteine protease-type cathepsin by singlet oxygen generated from naphthalene endoperoxides.
Biochem Biophys Res Commun
2005
331:215-23
PubMed ID: 15845381
DOI: 10.1016/j.bbrc.2005.03.146
|
9611
Y Hagihara, Y Saitoh, H Iwata, T Taki, S Hirano, N Arita, T Hayakawa
Transplantation of Xenogeneic Cells Secreting Beta-Endorphin for Pain Treatment: Analysis of the Ability of Components of Complement to Penetrate Through Polymer Capsules
Cell Transplant
1997
6(5):527-30
PubMed ID: 9331506
DOI: 10.1177/096368979700600515
|