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Cell No. : Cell Name
RCB0690 : HOTHC-SF  update : 2022/03/22
CommentA subline of HOTHC. Serum-free and protein-free culture is possible.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, Isamu
Year of deposit 1991
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Tissue thyroid
Disease name anaplastic thyroid carcinoma
Classification cancer
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_8709
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/2-4 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium HamF12 : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Reference information Reference 1
User's Publication 0

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1524  Ono I, Ishiwata I, Nakaguchi T, Konoe K, Tokita N, Soma M, Ishiwata C, Ishikawa H  [Heterotransplantability of human anaplastic thyroid carcinoma line (HOTHC) in nude mice, and biological properties of the heterograft]  Hum Cell  1989  2:74-9  PubMed ID: 2519207

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