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Cell No. : Cell Name
RCB0689 : TCO-2  update : 2022/07/07
CommentHuman cell line derived from uterine cervix cancer. Adenocarcinoma. Said producing CEA, CA125, TPA.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Izumi, Rikuichi
Originator Fushiki, Hiroshi
Year of deposit 1991
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 34 years
Tissue cervix, lymph node meta
Disease name uterine cervical adenocarcinom
Metastatic ability No
Classification cancer
Year of origin 1987
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_3630
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 45-93(50) : 45(7),46(38),47(1),90(1),92(3)
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 4

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54  Fushiki H.  Characterization of two novel cell lines established from a single human uterine cervical adenocarcinoma, and their chemosensitivities and hormonal effects on their proliferation  Nihon Sanka Fujinka Gakkai Zasshi  1992  44(4):397-404  PubMed ID: 1607768  

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User's Publication
14363  Takiguchi E, Nishimura M, Mineda A, Kawakita T, Abe A, Irahara M.  Growth inhibitory effect of the Src inhibitor dasatinib in combination with anticancer agents on uterine cervical adenocarcinoma cells  Exp Ther Med  2017  14(5):4293-4299  PubMed ID: 29067110   DOI: 10.3892/etm.2017.5061
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x
2706  Harada H, Watanabe S, Saijo K, Ishiwata I, Ohno T  A Wilms tumor cell line, HFWT, can greatly stimulate proliferation of CD56+ human natural killer cells and their novel precursors in blood mononuclear cells.  Exp Hematol  2004  32:614-21  PubMed ID: 15246157   DOI: 10.1016/j.exphem.2004.03.011
2758  Harada, Hideki, Saijo, Kaoru, Watanabe, Satoru, Tsuboi, Koji, Nose, Tadao, Ishiwata, Isamu, Ohno, Tadao  Selective expansion of human natural killer cells from peripheral blood mononuclear cells by the cell line, HFWT.  Jpn J Cancer Res  2002  93:313-9  PubMed ID: 11927014   DOI: 10.1111/j.1349-7006.2002.tb02174.x

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