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Cell No. : Cell Name
RCB0688 : QG-U  update : 2022/07/07
CommentJapanese cervix carcinoma.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Mashiba, Harukazu
Originator Mashiba, Harukazu
Year of deposit 1991
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Tissue cervix
Disease name cervix carcinoma
Classification cancer
Year of origin 1974
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_3778
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives M199 + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 72-154(50) : 73(4),74(8),75(8),76(6),77(9),78(10)/
STR(human) OK
Reference information Reference 1
User's Publication 4

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2568  Ishiwata I, Yoshikawa H, Nakagawa S, Kiguchi K, Ishikawa H  Human papillomavirus types 56 and 59 in novel cervical cancer cell lines.  Hum Cell  2000  13:55-6  PubMed ID: 10937347  

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User's Publication
5011  Nagura M, Matsumura N, Baba T, Murakami R, Kharma B, Hamanishi J, Yamaguchi K, Abiko K, Koshiyama M, Mandai M, Murata T, Murphy SK, Konishi I.  Invasion of uterine cervical squamous cell carcinoma cells is facilitated by locoregional interaction with cancer-associated fibroblasts via activating transforming growth factor-beta.  Gynecol Oncol  2015  136(1):104-11  PubMed ID: 25434636   DOI: 10.1016/j.ygyno.2014.11.075
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x
17338  Suzuki T, Takimoto K.  Selective expression of HERG and Kv2 channels influences proliferation of uterine cancer cells  Int J Oncol  2004  25(1):153-9  PubMed ID: 15202000  
17252  Masa-Aki Okubo, Akira Matsuno, Masayoshi Namba, Hiroshi Hosoya  A monoclonal antibody, mH1, specifically recognizes microvilli of dividing HeLa cells  Tiss Cult Res Commum  1999  18:113-117    DOI: 10.11418/jtca1981.18.1_113

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