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Cell No. : Cell Name
RCB0687 : KP-N-NS  update : 2022/04/15
CommentAdrenal neuroblastoma derived from brain metastasis
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yoshihara, Takao
Originator Yoshihara, Takao
Year of deposit 1991
Another name human neuroblastoma
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 1 year
Tissue adrenal, brain meta
Disease name neuroblastoma
Metastatic ability Yes
Metastatic tissue brain, bone marrow
Classification cancer
Year of origin 1990
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_4771
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/7-10 days, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 45-92(50) : 45(6),46(38),47(5),92(1)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
1922  Yoshihara T, Ikushima S, Hibi S, Misawa S, Imashuku S  Establishment and characterization of a human neuroblastoma cell line derived from a brain metastatic lesion.  Hum Cell  1993  6:210-7  PubMed ID: 8297818  

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User's Publication
15591  Miyake I, Hakomori Y, Shinohara A, Gamou T, Saito M, Iwamatsu A, Sakai R.  Activation of anaplastic lymphoma kinase is responsible for hyperphosphorylation of ShcC in neuroblastoma cell lines  Oncogene  2002  21(38):5823-34  PubMed ID: 12185581   DOI: 10.1038/sj.onc.1205735



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