Cell No. : Cell Name
RCB0685 : SKG-II-SF
update : 2022/03/22
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Comment | A subline of SKG-II (human cell line derived from uterine cervix cancer, large cell non-keratinizing squamous cell carcinoma). Serum-free culture is possible. HPV type 18 integrated. |
Comment from the depositor | |
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Ishiwata, Isamu
|
Originator |
Ishiwata, Isamu
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Year of deposit |
1991
|
Animal |
_human
< Mammals
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Genus |
Homo
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Species |
sapiens
|
Gender |
Female
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Age at sampling |
50 years
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Tissue |
cervix
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Disease name |
Squamouse carcinoma
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Classification |
cancer
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|
Lifespan |
infinite
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Morphology |
epithelial-like
|
Cellosaurus(Expasy) |
CVCL_8159
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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Culture medium |
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HamF12
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Antibiotics |
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Free
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|
Passage method |
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0.25% Trypsin, washed twice with medium, then seeded
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Culture information
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Passage ratio |
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1 : 4 split
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SC frequency |
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Subculture : once/10 days, Medium Renewal : 2 times/week
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Temperature |
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37
℃
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CO2 concentration |
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5
%
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Freeze medium |
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HamF12 : 2 x FM1 = 1 : 1
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Freezing method |
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Slow freezing
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Mycoplasma |
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(-)
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Virus (HIV) |
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(-)
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Isozyme |
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LD, NP, MPI, PepB
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STR(human) |
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OK
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Images |
deposit info | lot info |
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Reference information |
Reference |
6
|
User's Publication |
2
|
Reference |
935
Yamamoto M, Hashimoto H, Tachibana T, Ohi S, Akahori M, Yokose T, Ishiwata I, Ishikawa H.
New method for forming large embryoid bodies using the wall of the culture dish along with an analysis of their structural characteristics
Hum Cell
2004
17(1):49-57
PubMed ID: 15369137
DOI: 10.1111/j.1749-0774.2004.tb00020.x
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934
Yamamoto M, Tachibana T, Hashimoto H, Ishiwata I, Ishikawa H.
The differentiation of early embryonic stem cells into adipocytes-like cells
Hum Cell
2003
16(3):117-22
PubMed ID: 15005242
DOI: 10.1111/j.1749-0774.2003.tb00143.x
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2565
Ishiwata I, Tokieda Y, Kiguchi K, Sato K, Ishikawa H
Effects of embryotrophic factors on the embryogenesis and organogenesis of mouse embryos in vitro.
Hum Cell
2000
13:185-95
PubMed ID: 11329934
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2567
Ishiwata I, Tokieda Y, Ishiwata C, Okane N, Iguchi M, Sato K, Ishikawa H.
Effects of feeder cells (human cancer cell lines) on the development of mouse embryos by co-culture.
Hum Cell
1997
10(4):237-46
PubMed ID: 9573483
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2566
Ishiwata I, Ishiwata C, Soma M, Akagi H, Hiyama H, Nakaguchi T, Ono I, Hashimoto H, Ishikawa H.
Presence of human papillomavirus genome in human tumor cell lines and cultured cells.
Anal Quant Cytol Histol
1991
13(5):363-70
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1200
tshiwata,L, IVbeawa,S..Kignchi, Kl, Khrriham, S. and Oleumura. H
Establishment of human uterine cervical cancer cell line(SKG-2,2B) and comparative studies between normal and malignant uterine cervical cells in vitro.
Acta Obst Gynaec Jpn
1978
3e:731
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User's Publication |
6410
Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.
Is parainfluenza virus a threatening virus for human cancer cell lines?
Hum Cell
2009
22(3):81-4
PubMed ID: 19624309
DOI: 10.1111/j.1749-0774.2009.00071.x
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3335
Yoshino K, Iimura E, Saijo K, Iwase S, Fukami K, Ohno T, Obata Y, Nakamura Y.
Essential role for gene profiling analysis in the authentication of human cell lines.
Hum Cell
2006
19(1):43-8
PubMed ID: 16643607
DOI: 10.1111/j.1749-0774.2005.00007.x
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