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Cell No. : Cell Name
RCB0685 : SKG-II-SF  update : 2022/03/22
CommentA subline of SKG-II (human cell line derived from uterine cervix cancer, large cell non-keratinizing squamous cell carcinoma). Serum-free culture is possible. HPV type 18 integrated.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, Isamu
Year of deposit 1991
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 50 years
Tissue cervix
Disease name Squamouse carcinoma
Classification cancer
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_8159
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF12
Antibiotics Free
Passage method 0.25% Trypsin, washed twice with medium, then seeded
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/10 days, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium HamF12 : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP, MPI, PepB
STR(human) OK
deposit info
lot info
Reference information Reference 6
User's Publication 2

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935  Yamamoto M, Hashimoto H, Tachibana T, Ohi S, Akahori M, Yokose T, Ishiwata I, Ishikawa H.  New method for forming large embryoid bodies using the wall of the culture dish along with an analysis of their structural characteristics  Hum Cell  2004  17(1):49-57  PubMed ID: 15369137   DOI: 10.1111/j.1749-0774.2004.tb00020.x
934  Yamamoto M, Tachibana T, Hashimoto H, Ishiwata I, Ishikawa H.  The differentiation of early embryonic stem cells into adipocytes-like cells  Hum Cell  2003  16(3):117-22  PubMed ID: 15005242   DOI: 10.1111/j.1749-0774.2003.tb00143.x
2565  Ishiwata I, Tokieda Y, Kiguchi K, Sato K, Ishikawa H  Effects of embryotrophic factors on the embryogenesis and organogenesis of mouse embryos in vitro.  Hum Cell  2000  13:185-95  PubMed ID: 11329934  
2567  Ishiwata I, Tokieda Y, Ishiwata C, Okane N, Iguchi M, Sato K, Ishikawa H.  Effects of feeder cells (human cancer cell lines) on the development of mouse embryos by co-culture.  Hum Cell  1997  10(4):237-46  PubMed ID: 9573483  
2566  Ishiwata I, Ishiwata C, Soma M, Akagi H, Hiyama H, Nakaguchi T, Ono I, Hashimoto H, Ishikawa H.  Presence of human papillomavirus genome in human tumor cell lines and cultured cells.  Anal Quant Cytol Histol  1991  13(5):363-70   
1200  tshiwata,L, IVbeawa,S..Kignchi, Kl, Khrriham, S. and Oleumura. H  Establishment of human uterine cervical cancer cell line(SKG-2,2B) and comparative studies between normal and malignant uterine cervical cells in vitro.  Acta Obst Gynaec Jpn  1978  3e:731   

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User's Publication
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x
3335  Yoshino K, Iimura E, Saijo K, Iwase S, Fukami K, Ohno T, Obata Y, Nakamura Y.  Essential role for gene profiling analysis in the authentication of human cell lines.  Hum Cell  2006  19(1):43-8  PubMed ID: 16643607   DOI: 10.1111/j.1749-0774.2005.00007.x

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