Cell No. : Cell Name
RCB0684 : HFL-AE-III
update : 2025/02/03
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| Comment | Human fibroblasts derived from lung of anencephalic fetus (15 weekly age). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Ishiwata, Isamu
|
| Originator |
Ishiwata, Isamu
|
| Year of deposit |
1991
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Unknown
|
| Age at sampling |
15 weeks embryo
|
| Tissue |
embryo/fetus, lung
|
| Classification |
normal
|
| Year of origin |
1991
|
|
Lifespan |
finite
|
| Morphology |
fibroblast-like
|
| Contact inhibition |
Yes
|
| Cellosaurus(Expasy) |
CVCL_4638
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
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HamF12 + 15% FBS
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| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
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Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HIV) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
45-92(50) : 45(2),46(46),47(1),92(1)
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
0
|
| User's Publication |
2
|
| User's Publication |
|
22524
Kasai F, Fukushima M, Miyagi Y, Nakamura Y.
Genetic diversity among the present Japanese population: evidence from genotyping of human cell lines established in Japan
Hum Cell
2024
37(4):944-950
PubMed ID: 38639832
DOI: 10.1007/s13577-024-01055-0
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3579
Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y.
Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.
Stem Cells
2007
25(7):1610-7
PubMed ID: 17395773
DOI: 10.1634/stemcells.2006-0504
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