Search
CELL BANK Website

Back Back
Cell No. : Cell Name
RCB0679 : HDC  update : 2022/03/22
CommentHuman fibroblasts derived from uterine. 46XX,t(2/q2/q). The first child of this donor suffers from Down's syndrome, 46XX,t(2/q2/q).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, Isamu & Ishikawa, Hiroshi
Year of deposit 1991
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 29 years
Tissue cervix
Classification normal
Year of origin 1991
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_K098
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 43-90(50) : 43(1),44(4),45(41),46(1),90(3)
STR(human) OK
Reference information Reference 0
User's Publication 0


To topTop
Reference

To topTop
User's Publication



Back Back Return Top Page