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Cell No. : Cell Name
RCB0676 : PCH42-58  update : 2022/03/22
CommentHybridoma producing monoclonal antibody against differentiated PC-12 cell surface molecules.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kuroda, Y. & Kobayashi, K.
Originator Kobayashi, Kazuo
Year of deposit 1991
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_K259
hybridoma Fusion_cell_1 name spleen cell
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name P3-X63-Ag8.653
Fusion_cell_2 strain mouse
Fusion method PEG #4000
Antibody name PCH42-58
Subclass IgM
Immunogen PC-12 cells differentiated with NGF.
Antigen_name surface antigen of PC-12 cells
Antigen_character surface antigen of PC-12 cells
Antigen_positive hippocampus of rat
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 15% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgM
Images
deposit info
lot info
Reference information Reference 3
User's Publication 0


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Reference
1438  Ariga T, Suzuki M, Yu RK, Kuroda Y, Shimada I, Inagaki F, Miyatake T.  Accumulation of unique globo-series glycolipids in PC 12h pheochromocytoma cells.  J Biol Chem  1989  264(3):1516-21  PubMed ID: 2912970  
1437  Kuroda Y, Kobayashi K, Ohguchi Y.  A library of monoclonal antibodies for exploring unknown functional molecules on the surface of synaptic membrane.  Adv Exp Med Biol  1988  236:153-61  PubMed ID: 2853561   DOI: 10.1007/978-1-4757-5971-6_12
1439  Ariga T, Kobayashi K, Kuroda Y, Yu RK, Suzuki M, Kitagawa H, Inagaki F, Miyatake T.  Characterization of tumor-associated fucogangliosides from PC 12 pheochromocytoma cells.  J Biol Chem  1987  262(29):14146-53  PubMed ID: 3654655  

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