Cell No. : Cell Name
RCB0673 : PCH41-43
update : 2024/01/17
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| Comment | Hybridoma against differentiated PC-12 cell surface |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Kuroda, Y. & Kobayashi, K.
|
| Originator |
Kobayashi, Kazuo
|
| Year of deposit |
1991
|
| Animal |
_mouse x mouse
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_K257
|
|
hybridoma
|
Fusion_cell_1 name |
spleen cell
|
| Fusion_cell_1 strain |
mouse, BALB/c
|
| Fusion_cell_2 name |
P3-X63-Ag8.653
|
| Fusion_cell_2 strain |
mouse
|
|
Fusion method |
PEG #4000
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|
Antibody name |
PCH41-43
|
| Subclass |
IgM
|
| Immunogen |
PC-12 cells differentiated with NGF.
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|
Antigen_name |
surface antigen of PC-12 cells
|
| Antigen_character |
surface antigen of PC-12 cells
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|
Antigen_positive |
Extracellular matrix
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|
| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 + 15% FBS
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| Antibiotics |
|
Free
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|
Passage method |
|
dilution
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Culture information
|
Passage ratio |
|
1 : 10 split
|
| SC frequency |
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Subculture : 2 times/week
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| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Antibody subclass |
|
IgM
|
| Reference information |
Reference |
3
|
| User's Publication |
0
|
| Reference |
|
1438
Ariga T, Suzuki M, Yu RK, Kuroda Y, Shimada I, Inagaki F, Miyatake T.
Accumulation of unique globo-series glycolipids in PC 12h pheochromocytoma cells.
J Biol Chem
1989
264(3):1516-21
PubMed ID: 2912970
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1437
Kuroda Y, Kobayashi K, Ohguchi Y.
A library of monoclonal antibodies for exploring unknown functional molecules on the surface of synaptic membrane.
Adv Exp Med Biol
1988
236:153-61
PubMed ID: 2853561
DOI: 10.1007/978-1-4757-5971-6_12
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1439
Ariga T, Kobayashi K, Kuroda Y, Yu RK, Suzuki M, Kitagawa H, Inagaki F, Miyatake T.
Characterization of tumor-associated fucogangliosides from PC 12 pheochromocytoma cells.
J Biol Chem
1987
262(29):14146-53
PubMed ID: 3654655
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