Cell No. : Cell Name
RCB0666 : HSNB
update : 2024/11/12
|
| Comment | Human cell line derived from Neuroblastoma. N-myc gene is amplified. Producing neuron specific enolase.Application consideration |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
Ishiwata, Isamu
|
| Originator |
Ono, Isao & Ishiwata, Isamu
|
| Year of deposit |
1991
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Male
|
| Age at sampling |
9 months
|
| Tissue |
adrenal
|
| Disease name |
neuroblastoma
|
| Classification |
cancer
|
| Year of origin |
1982
|
|
Lifespan |
infinite
|
| Morphology |
other
|
| Cellosaurus(Expasy) |
CVCL_L554
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
HamF12 + 15% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 3 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HIV) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
40-92(50) : /42(2),43(5),44(11),45(11),46(16),88(1)/
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
2
|
| User's Publication |
0
|
| Reference |
|
1527
Ono I, Ishiwata I, Nakaguchi T, Ohara K, Tokita N, Soma M, Ishiwata C, Nozawa S, Ishikawa H
[Establishment and characterization of human neuroblastoma cell line (HSNB)]
Hum Cell
1989
2:416-22
PubMed ID: 2486666
|
|
48
Ishiwata I, Ishiwata C, Soma M, Ono I, Nakaguchi T, Nozawa S, Ishikawa H.
N-myc amplification and neuron-specific enolase production of a neuroblastoma cell line and germ cell tumor cell lines
Gynecol Oncol
1989
33(3):356-9
PubMed ID: 2542141
DOI: 10.1016/0090-8258(89)90527-1
|
Back
Top
CELL BANK Website
