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Cell No. : Cell Name
RCB0666 : HSNB  update : 2022/04/15
CommentHuman cell line derived from Neuroblastoma. N-myc gene is amplified. Producing neuron specific enolase.Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ishiwata, Isamu
Originator Ono, Isao & Ishiwata, Isamu
Year of deposit 1991
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 9 months
Tissue adrenal
Disease name neuroblastoma
Classification cancer
Year of origin 1982
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_L554
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives HamF12 + 15% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 3 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 40-92(50) : /42(2),43(5),44(11),45(11),46(16),88(1)/
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 0

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1527  Ono I, Ishiwata I, Nakaguchi T, Ohara K, Tokita N, Soma M, Ishiwata C, Nozawa S, Ishikawa H  [Establishment and characterization of human neuroblastoma cell line (HSNB)]  Hum Cell  1989  2:416-22  PubMed ID: 2486666  

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