Cell No. : Cell Name
RCB0665 : HFWT update : 2022/03/30 |
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Comment | Human cell line derived from Wilms' tumor. Expressing CA125 and TPA. | |||
Comment from the depositor | ||||
Terms and conditions | Need an approval of the approver. Academic basic research purpose only (excluding any type of commercial use, i.e., commercializing not only HFWT cells but also all the HFWT-originated commercially useful materials and methods such as the cellular components, products, genes, their derivatives, application methods, and the like.) . | |||
Remarks | ||||
approver's address |
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Order Form | Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   | |||
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
Basic information | Depositor | Ishiwata, Isamu (Ohno, Tadao) | ||
Originator | Ono, I. & Ishiwata, I. & Ishikawa, H. | |||
Year of deposit | 1991 | |||
Animal | human < Mammals | |||
Genus | Homo | |||
Species | sapiens | |||
Gender | Male | |||
Age at sampling | 5 months | |||
Tissue | kidney | |||
Disease name | Wilms' tumor | |||
Classification | cancer | |||
Year of origin | 1984 | Lifespan | infinite | |
Morphology | epithelial-like | |||
Cellosaurus(Expasy) | CVCL_8691 | |||
Medium | Medium List | Culture type | Adherent cells | |
Medium and additives | HamF12 + 15% FBS | |||
Antibiotics | Free | Passage method | 0.25% Trypsin | Culture information | Passage ratio | 1 : 8 split |
SC frequency | Subculture : once/week, Medium Renewal : 2 times/week | |||
Temperature | 37 ℃ | |||
CO2 concentration | 5 % | |||
Freeze medium | Medium + 10% DMSO | |||
Freezing method | Slow freezing | |||
Mycoplasma | (-) | |||
Virus (HIV) | (-) | |||
Isozyme | LD, NP | |||
Chromosome mode | 44-92(50) : 44(2),45(8),46(35),47(3),92(2)/ | |||
STR(human) | OK | |||
Reference information | Reference | 1 | ||
User's Publication | 7 | |||