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Cell No. : Cell Name
RCB0662 : HOTHC  update : 2022/07/22
CommentHuman cell line derived from undifferentiated carcinoma of thyroid (pleomorphous). Expressing tumor angiogenesis factor (TAF) and G-CSF.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ishiwata, Isamu
Originator Ono, I. & Ishiwata, I. & Ishikawa, H.
Year of deposit 1991
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue thyroid
Disease name anaplastic thyroid carcinoma
Classification cancer
Year of origin 1982
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_8708
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF12 + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 66-164(50) : /79(6),80(8),81(3),82(8),83(1),84(3)/
STR(human) OK
deposit info
lot info
Reference information Reference 3
User's Publication 2

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860  shiwata I, Ono I, Kiguchi K, Ishiwata C, Soma M, Ishikawa H.  Establishment and characterization of a human thyroid carcinoma cell line (HOTHC) producing colony stimulating factor  Hum Cell  2005  18(3):163-9  PubMed ID: 17022149   DOI: 10.1111/j.1749-0774.2005.tb00007.x
47  Ishiwata I, Sudo T, Kiguchi K, Ishikawa H.  Tumor angiogenesis factors produced by cancer cells  Hum Cell  1999  ;12(1):37-4  PubMed ID: 10457904  
1524  Ono I, Ishiwata I, Nakaguchi T, Konoe K, Tokita N, Soma M, Ishiwata C, Ishikawa H  [Heterotransplantability of human anaplastic thyroid carcinoma line (HOTHC) in nude mice, and biological properties of the heterograft]  Hum Cell  1989  2:74-9  PubMed ID: 2519207  

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User's Publication
16175  Bonaldi E, Gargiuli C, De Cecco L, Micali A, Rizzetti MG, Greco A, Borrello MG, Minna E.  BRAF Inhibitors Induce Feedback Activation of RAS Pathway in Thyroid Cancer Cells  Int J Mol Sci  2021  22(11):5744  PubMed ID: 34072194   DOI: 10.3390/ijms22115744
1902  Shu Qin Liu, Kaoru Saijo, Makoto Kodama, Tadao Ohno  Intelligent matrices for tumor-specific cytotoxic T lymphocytes  Materials Science and Engineering: C  1994  2:61-65    DOI: 10.1016/0928-4931(94)90030-2

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