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Cell No. : Cell Name
RCB0660 : HTMMT  update : 2023/01/25
CommentHuman cell line derived from malignant mixed mesodermal tumor found in uterus (malignant mixed Müllerian tumor).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, I. & Ishikawa, H.
Year of deposit 1991
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 66 years
Tissue endometrium
Disease name Mixed Mullerian tumor
Classification cancer
Year of origin 1987
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_8721
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF12 + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 60-143(50) : /67(2),68(3),69(3),70(5),71(8),72(10)/
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 1

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931  Ishiwata I, Ishiwata C, Soma M, Iguchi M, Kiguchi K, Tamagawa T, Ishiwata I, Ishikawa H.  Histogenesis of carcinosarcoma and establishment of leiomyosarcoma cell line (HTMMT) derived from human uterine carcinosarcoma  Hum Cell  2004  17(3):131-7.  PubMed ID: 15859158   DOI: 10.1111/j.1749-0774.2004.tb00029.x

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User's Publication
14017  Tanaka T, Bai T, Yukawa K.  Death-associated protein kinase is essential for the survival of various types of uterine cancer cells  Int J Oncol  2010  37(4):1017-22  PubMed ID: 20811724   DOI: 10.3892/ijo_00000753

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