Back Back
Cell No. : Cell Name
RCB0659 : HOUA-I  update : 2022/07/07
CommentHuman cell line derived from uterine cancer (endometrial cancer).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, Isamu
Year of deposit 1991
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 55 years
Tissue endometrium
Disease name Endometrial adenocarcinoma
Classification cancer
Year of origin 1982
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_3867
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF12 + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/3-4 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 43-92(50) : /44(1),45(10),46(19),47(3),48(3),49(1)/
STR(human) OK
deposit info
lot info
Reference information Reference 5
User's Publication 10

To topTop
41  Ishiwata I, Ishiwata C, Nozawa S, Ishikawa H.  CA125 production by gynecologic tumors in vitro and its modulation induced by dibutyl cyclic adenosine monophosphate  Asia Oceania J Obstet Gynaecol  1996  12(2):285-90  PubMed ID: 2429643   DOI: 10.1111/j.1447-0756.1986.tb00192.x
46  Nakai T, Endo K, Hosono M, Saga T, Watanabe Y, Sakahara H, Imai K, Yachi A, Kiyozuka Y, Ishiwata I.  Drug effects on CA125 antigen expression and antibody binding to cancer cells  Int J Cancer  1991  48(3):463-7  PubMed ID: 1645702   DOI: 10.1002/ijc.2910480326
45  Saga T, Ishiwata I, Endo K, Sakahara H, Koizumi M, Watanabe Y, Nakai T, Hosono M, Ishikawa H, Sawada M.  An antibody-tumor model for the targeting of CA125-producing gynecologic malignancies  Jpn J Cancer Res  1990  81(11):1141-8  PubMed ID: 2125039   DOI: 10.1111/j.1349-7006.1990.tb02526.x
1522  Ishieata, I., Soma, M., Ono, I., Nakaguchi, T., Ishiwata, C., Nozawa, S.and Ishikawa, H.  Biological Characterristics of Adenocarcinoma Cell Line ( HOUA-I ) and B-Lymphoblastoid Cell Line ( HOUA-II ) Established from The Same Tissue of Endmetrial Adenocarcinoma  Jikeikai Medical Journal  1989  36:303-315   
1035  Ishiwata I, Ishiwata C, Soma M, Ono I, Nakaguchi T, Ishikawa H.  Tumor angiogenic activity of gynecologic tumor cell lines on the chorioallantoic membrane  Gynecol Oncol  1988  29(1):87-93  PubMed ID: 2448191   DOI: 10.1016/0090-8258(88)90151-5

To topTop
User's Publication
19737  Yagi H, Onoyama I, Asanoma K, Kawakami M, Maenohara S, Kodama K, Matsumura Y, Hamada N, Hori E, Hachisuga K, Yasunaga M, Ohgami T, Okugawa K, Yahata H, Kato K.  Tumor-derived ARHGAP35 mutations enhance the Gα13-Rho signaling axis in human endometrial cancer  Cancer Gene Ther  2023  30(2):313-323  PubMed ID: 36257976   DOI: 10.1038/s41417-022-00547-1
13927  Xu D, Dong P, Xiong Y, Chen R, Konno Y, Ihira K, Yue J, Watari H.  PD-L1 Is a Tumor Suppressor in Aggressive Endometrial Cancer Cells and Its Expression Is Regulated by miR-216a and lncRNA MEG3  Front Cell Dev Biol  2020  8:598205  PubMed ID: 33363153   DOI: 10.3389/fcell.2020.598205
14241  Ihira K, Dong P, Xiong Y, Watari H, Konno Y, Hanley SJ, Noguchi M, Hirata N, Suizu F, Yamada T, Kudo M, Sakuragi N.  EZH2 inhibition suppresses endometrial cancer progression via miR-361/Twist axis  Oncotarget  2017  8(8):13509-13520  PubMed ID: 28088786   DOI: 10.18632/oncotarget.14586
17129  Dong P, Kaneuchi M, Watari H, Sudo S, Sakuragi N.  MicroRNA-106b modulates epithelial-mesenchymal transition by targeting TWIST1 in invasive endometrial cancer cell lines  Mol Carcinog  2014  53(5):349-59  PubMed ID: 24002805   DOI: 10.1002/mc.21983
7708  Dong P, Kaneuchi M, Watari H, Hamada J, Sudo S, Ju J, Sakuragi N.  MicroRNA-194 inhibits epithelial to mesenchymal transition of endometrial cancer cells by targeting oncogene BMI-1.  Mol. Cancer  2011  10:99  PubMed ID: 21851624   DOI: 10.1186/1476-4598-10-99
8772  Sugimoto T, Koizumi T, Sudo T, Yamaguchi S, Kojima A, Kumagai S, Nishimura R.  Correlative expression of cyclooxygenase-1 (Cox-1) and human epidermal growth factor receptor type-2 (Her-2) in endometrial cancer.  Kobe J Med Sci  2007  53:177-87  PubMed ID: 18204294  
13795  Sugawara T, Nomura E, Fujimoto S.  Expression of enzyme associated with steroid hormone synthesis and local production of steroid hormone in endometrial carcinoma cells  J Endocrinol  2004  180(1):135-44  PubMed ID: 14709152   DOI: 10.1677/joe.0.1800135
13831  Unoki M, Nakamura Y.  Methylation at CpG islands in intron 1 of EGR2 confers enhancer-like activity  FEBS Lett  2003  554(1-2):67-72  PubMed ID: 14596916   DOI: 10.1016/s0014-5793(03)01092-5
3101  Matsushima-Nishiu M, Unoki M, Ono K, Tsunoda T, Minaguchi T, Kuramoto H, Nishida M, Satoh T, Tanaka T, Nakamura Y  Growth and gene expression profile analyses of endometrial cancer cells expressing exogenous PTEN.  Cancer Res  2001  61:3741-9  PubMed ID: 11325847  
1902  Shu Qin Liu, Kaoru Saijo, Makoto Kodama, Tadao Ohno  Intelligent matrices for tumor-specific cytotoxic T lymphocytes  Materials Science and Engineering: C  1994  2:61-65    DOI: 10.1016/0928-4931(94)90030-2

Back Back Return Top Page