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Cell No. : Cell Name
RCB0658 : HHUA  update : 2021/05/20
CommentHuman cell line derived from endometrial cancer. Expressing receptor of estradiol-17beta and progesterone.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, Isamu
Year of deposit 1991
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 54 years
Tissue endometrium
Disease name Endometrial adenocarcinoma
Classification cancer
Year of origin 1980
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_3866
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 15% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/1-2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 40-92(50) : /44(2),45(7),46(29),47(6),53(1),92(2)
STR(human) OK
deposit info
lot info
Reference information Reference 2
User's Publication 68

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1521  Ishiwata I, Ishiwata C, Ishikawa H  Effects of estradiol-17 beta and progesterone on cell proliferation and differentiation of the human endometrial carcinoma cell line (HHUA) in vitro.  Asia Oceania J Obstet Gynaecol  1984  10:531-8  PubMed ID: 6532401
1519  Ishiwata I, Ishiwata C, Soma M, Arai J, Ishikawa H  Establishment of human endometrial adenocarcinoma cell line containing estradiol-17 beta and progesterone receptors.  Gynecol Oncol  1984  17:281-90  PubMed ID: 6706228

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User's Publication
13789  Kojima M, Sugimoto K, Kobayashi M, Ichikawa-Tomikawa N, Kashiwagi K, Watanabe T, Soeda S, Fujimori K, Chiba H.  Aberrant claudin-6-adhesion signaling promotes endometrial cancer progression via estrogen receptor α  Mol Cancer Res  2021  molcanres.0835.2020  PubMed ID: 33727343
14469  Yusuke Hanaki, Yusuke Araki, Toshio Nishikawa, Ryo C. Yanagita  Evaluation of the in vitro cytotoxicity of oscillatoxins E and F under nutrient-starvation culture conditions  Fundamental Toxicological Sciences  2021  8:69-73 
20209  Dong P, Xiong Y, Konno Y, Ihira K, Kobayashi N, Yue J, Watari H.  Long non-coding RNA DLEU2 drives EMT and glycolysis in endometrial cancer through HK2 by competitively binding with miR-455 and by modulating the EZH2/miR-181a pathway.  J Exp Clin Cancer Res  2021  40(1):216  PubMed ID: 34174908
13321  Kato M, Onoyama I, Kawakami M, Yoshida S, Kawamura K, Kodama K, Hori E, Cui L, Matsumura Y, Yagi H, Asanoma K, Yahata H, Itakura A, Takeda S, Kato K.  Downregulation of 5-hydroxymethylcytosine is associated with the progression of cervical intraepithelial neoplasia.  PLoS One  2020    PubMed ID: 33141854
13779  Kato M, Onoyama I, Yoshida S, Cui L, Kawamura K, Kodama K, Hori E, Matsumura Y, Yagi H, Asanoma K, Yahata H, Itakura A, Takeda S, Kato K.  Dual-specificity phosphatase 6 plays a critical role in the maintenance of a cancer stem-like cell phenotype in human endometrial cancer  Int J Cancer  2020  147(7):1987-1999  PubMed ID: 32159851
10248  Osanai K, Kobayashi Y, Otsu M, Izawa T, Sakai K, Iwashita M.  Ramelteon, a selective MT1/MT2 receptor agonist, suppresses the proliferation and invasiveness of endometrial cancer cells.  Hum. Cell  2017  30:209-215  PubMed ID: 28382516
10315  Ando H, Miyamoto T, Kashima H, Higuchi S, Ida K, Mvunta DH, Shiozawa T.  Panobinostat Enhances Growth Suppressive Effects of Progestin on Endometrial Carcinoma by Increasing Progesterone Receptor and Mitogen-Inducible Gene-6.  Horm Cancer  2017  8:257-267  PubMed ID: 28516379
9667  Chuwa AH, Sone K, Oda K, Ikeda Y, Fukuda T, Wada-Hiraike O, Inaba K, Makii C, Takeuchi M, Oki S, Miyasaka A, Kashiyama T, Arimoto T, Kuramoto H, Kawana K, Yano T, Osuga Y, Fujii T.  Significance of survivin as a prognostic factor and a therapeutic target in endometrial cancer.  Gynecol. Oncol.  2016  141:564-569  PubMed ID: 27079211
9687  Miyamoto T, Kashima H, Yamada Y, Kobara H, Asaka R, Ando H, Higuchi S, Ida K, Mvunta DH, Shiozawa T.  Lipocalin 2 Enhances Migration and Resistance against Cisplatin in Endometrial Carcinoma Cells.  PLoS ONE  2016  11:e0155220  PubMed ID: 27168162
12617  Nakayama K, Rahman MT, Rahman M, Nakamura K, Ishikawa M, Katagiri H, Sato E, Ishibashi T, Iida K, Ishikawa N, Kyo S.  CCNE1 amplification is associated with aggressive potential in endometrioid endometrial carcinomas  Int J Oncol  2016  48(2):506-16  PubMed ID: 26647729
12648  Zou J, Hong L, Luo C, Li Z, Zhu Y, Huang T, Zhang Y, Yuan H, Hu Y, Wen T, Zhuang W, Cai B, Zhang X, Huang J, Cheng J.  Metformin inhibits estrogen-dependent endometrial cancer cell growth by activating the AMPK-FOXO1 signal pathway  Cancer Sci  2016  107(12):1806-1817  PubMed ID: 27636742
7200  Asanoma K, Liu G, Yamane T, Miyanari Y, Takao T, Yagi H, Ohgami T, Ichinoe A, Sonoda K, Wake N, Kato K.  Regulation of the Mechanism of TWIST1 Transcription by BHLHE40 and BHLHE41 in Cancer Cells.  Mol. Cell. Biol.  2015  35:4096-109  PubMed ID: 26391953
7212  Asaka R, Miyamoto T, Yamada Y, Ando H, Mvunta DH, Kobara H, Shiozawa T.  Sirtuin 1 promotes the growth and cisplatin resistance of endometrial carcinoma cells: a novel therapeutic target.  Lab. Invest.  2015  95:1363-73  PubMed ID: 26367491
13796  Koyama N, Nishida Y, Ishii T, Yoshida T, Furukawa Y, Narahara H.  Telmisartan induces growth inhibition, DNA double-strand breaks and apoptosis in human endometrial cancer cells  PLoS One  2014  9(3):e93050  PubMed ID: 24667764
13828  Miyasaka A, Oda K, Ikeda Y, Wada-Hiraike O, Kashiyama T, Enomoto A, Hosoya N, Koso T, Fukuda T, Inaba K, Sone K, Uehara Y, Kurikawa R, Nagasaka K, Matsumoto Y, Arimoto T, Nakagawa S, Kuramoto H, Miyagawa K, Yano T, Kawana K, Osuga Y, Fujii T.  Anti-tumor activity of olaparib, a poly (ADP-ribose) polymerase (PARP) inhibitor, in cultured endometrial carcinoma cells  BMC Cancer  2014  14:179  PubMed ID: 24625059
6463  Li H, Narahara H.  15-Deoxy-Δ(12,14)-prostaglandin J(2) induces growth inhibition, cell cycle arrest and apoptosis in human endometrial cancer cell lines.  Int J Mol Med.  2013  31(4):778-88  PubMed ID: 23403520
13830  Ishii T, Kira N, Yoshida T, Narahara H.  Cucurbitacin D induces growth inhibition, cell cycle arrest, and apoptosis in human endometrial and ovarian cancer cells  Tumour Biol  2013  34(1):285-91  PubMed ID: 23150173
7588  Mitsuhashi Y, Horiuchi A, Miyamoto T, Kashima H, Suzuki A, Shiozawa T.  Prognostic significance of Notch signalling molecules and their involvement in the invasiveness of endometrial carcinoma cells.  Histopathology  2012  60:826-37  PubMed ID: 22348356
13801  Tanaka T, Bai T, Toujima S, Utsunomiya T, Utsunomiya H, Yukawa K, Tanaka J.  Impaired death-associated protein kinase-mediated survival signals in 5-fluorouracil-resistant human endometrial adenocarcinoma cells  Oncol Rep  2012  28(1):330-6  PubMed ID: 22552543
7708  Dong P, Kaneuchi M, Watari H, Hamada J, Sudo S, Ju J, Sakuragi N.  MicroRNA-194 inhibits epithelial to mesenchymal transition of endometrial cancer cells by targeting oncogene BMI-1.  Mol. Cancer  2011  10:99  PubMed ID: 21851624
7925  Miyamoto T, Kashima H, Suzuki A, Kikuchi N, Konishi I, Seki N, Shiozawa T.  Laser-captured microdissection-microarray analysis of the genes involved in endometrial carcinogenesis: stepwise up-regulation of lipocalin2 expression in normal and neoplastic endometria and its functional relevance.  Hum. Pathol.  2011  42:1265-74  PubMed ID: 21334721
13786  Honda K, Kajitani K, Nobeyama H, Kira Y, Yabunaka Y, Egami M, Zhi X, Fukuda T, Yoshida H, Matsumoto Y, Ichimura T, Yaui T, Sumi T, Ishiko O.  An upstream estrogen response element linked to exogenous p53 tumor suppressor gene expression differentiates effects of the codon 72 polymorphism  Asian Pac J Cancer Prev  2011  12(4):865-8  PubMed ID: 21790217
13815  Tanaka T, Bai T, Yukawa K.  Specific downregulation of death-associated protein kinase enhances Fas-mediated apoptosis in the human differentiated endometrial adenocarcinoma cell line, HHUA  Eur J Gynaecol Oncol  2011  32(3):293-6  PubMed ID: 21797120
13821  Tanaka T, Bai T, Utsunomiya H, Fukumoto T, Yukawa K.  STAT3 enhances intracellular Fas-mediated apoptotic signals in HHUA human endometrial epithelial cells  Mol Med Rep  2011  4(2):307-12  PubMed ID: 21468569
8350  Fakhry H, Miyamoto T, Kashima H, Suzuki A, Ke H, Konishi I, Shiozawa T.  Immunohistochemical detection of histone deacetylases in endometrial carcinoma: involvement of histone deacetylase 2 in the proliferation of endometrial carcinoma cells.  Hum. Pathol.  2010  41:848-58  PubMed ID: 20178884
13793  Bai T, Tanaka T, Yukawa K.  Targeted knockdown of death-associated protein kinase expression induces TRAIL-mediated apoptosis in human endometrial adenocarcinoma cells  Int J Oncol  2010  37(1):203-10  PubMed ID: 20514412
13794  Tanaka T, Bai T, Yukawa K.  Suppressed protein expression of the death-associated protein kinase enhances 5-fluorouracil-sensitivity but not etoposide-sensitivity in human endometrial adenocarcinoma cells  Oncol Rep  2010  24(5):1401-5  PubMed ID: 20878137
13827  Tanaka T, Bai T, Toujima S.  Establishment and characterization of monoclonal 5-fluorouracil-resistant cell lines derived from human endometrial adenocarcinoma  Int J Oncol   2010  37(3):731-6  PubMed ID: 20664942
13833  Takai N, Ueda T, Nishida M, Nasu K, Narahara H.  PK11195 enhances chemosensitivity to cisplatin and paclitaxel in human endometrial and ovarian cancer cells  Int J Mol Med  2010  25(1):97-103  PubMed ID: 19956907
13835  Takai N, Ueda T, Nishida M, Nasu K, Narahara H.  Synergistic anti-neoplastic effect of AG1478 in combination with cisplatin or paclitaxel on human endometrial and ovarian cancer cells  Mol Med Rep  2010  3(3):479-84  PubMed ID: 21472266
14016  Ishikawa M, Nakayama K, Yeasmin S, Katagiri A, Iida K, Nakayama N, Miyazaki K.  NAC1, a potential stem cell pluripotency factor expression in normal endometrium, endometrial hyperplasia and endometrial carcinoma  Int J Oncol  2010  36(5):1097-103  PubMed ID: 20372782
14017  Tanaka T, Bai T, Yukawa K.  Death-associated protein kinase is essential for the survival of various types of uterine cancer cells  Int J Oncol  2010  37(4):1017-22  PubMed ID: 20811724
14018  Li H, Takai N, Yuge A, Furukawa Y, Tsuno A, Tsukamoto Y, Kong S, Moriyama M, Narahara H.  Novel target genes responsive to the anti-growth activity of triptolide in endometrial and ovarian cancer cells  Cancer Lett  2010  297(2):198-206  PubMed ID: 20547442
8539  Suzuki A, Horiuchi A, Ashida T, Miyamoto T, Kashima H, Nikaido T, Konishi I, Shiozawa T.  Cyclin A2 confers cisplatin resistance to endometrial carcinoma cells via up-regulation of an Akt-binding protein, periplakin.  J. Cell. Mol. Med.  2009  14:2305-17  PubMed ID: 19583808
13787  Ishibashi M, Nakayama K, Yeasmin S, Katagiri A, Iida K, Nakayama N, Miyazaki K.  Expression of a BTB/POZ protein, NAC1, is essential for the proliferation of normal cyclic endometrial glandular cells and is up-regulated by estrogen  Clin Cancer Res  2009  15(3):804-11  PubMed ID: 19188150
13803  Takai N, Ueda T, Nasu K, Yamashita S, Toyofuku M, Narahara H.  Targeting calcium/calmodulin-dependence kinase I and II as a potential anti-proliferation remedy for endometrial carcinomas  Cancer Lett  2009  277(2):235-43  PubMed ID: 19168280
13822  Tanaka T, Wang C, Umesaki N.  Remodeling of the human endometrial epithelium is regulated by laminin and type IV collagen  Int J Mol Med  2009  23(2):173-80  PubMed ID: 19148540
8749  Matsumoto M, Yamaguchi Y, Seino Y, Hatakeyama A, Takei H, Niikura H, Ito K, Suzuki T, Sasano H, Yaegashi N, Hayashi S.  Estrogen signaling ability in human endometrial cancer through the cancer-stromal interaction.  Endocr. Relat. Cancer  2008  15:451-63  PubMed ID: 18508998
8772  Sugimoto T, Koizumi T, Sudo T, Yamaguchi S, Kojima A, Kumagai S, Nishimura R.  Correlative expression of cyclooxygenase-1 (Cox-1) and human epidermal growth factor receptor type-2 (Her-2) in endometrial cancer.  Kobe J Med Sci  2008  53:177-87  PubMed ID: 18204294
13798  Yoshizaki T, Enomoto T, Fujita M, Ueda Y, Miyatake T, Fujiwara K, Miyake T, Kimura T, Yoshino K, Kimura T.  Frequent inactivation of RUNX3 in endometrial carcinoma  Gynecol Oncol  2008  110(3):439-44  PubMed ID: 18572225
13823  Tanaka T, Umesaki N.  Leptin regulates the proliferation and apoptosis of human endometrial epithelial cells  Int J Mol Med  2008  22(5):683-9.  PubMed ID: 18949391
13829  Takai N, Ueda T, Nishida M, Nasu K, Narahara H.  Bufalin induces growth inhibition, cell cycle arrest and apoptosis in human endometrial and ovarian cancer cells  Int J Mol Med  2008  21(5):637-43  PubMed ID: 18425357
13834  Takai N, Ueda T, Nishida M, Nasu K, Narahara H.  K252a is highly effective in suppressing the growth of human endometrial cancer cells, but has little effect on normal human endometrial epithelial cells  Oncol Rep  2008  19(3):749-53  PubMed ID: 18288411
13785  Zhi X, Honda K, Sumi T, Yasui T, Nobeyama H, Yoshida H, Ishiko O.  Estradiol-17beta regulates vascular endothelial growth factor and Bcl-2 expression in HHUA cells  Int J Oncol  2007  31(6):1333-8  PubMed ID: 17982659
13790  Abe H, Shibata MA, Otsuki Y.  Caspase cascade of Fas-mediated apoptosis in human normal endometrium and endometrial carcinoma cells  Mol Hum Reprod  2006  12(9):535-41  PubMed ID: 16870953
3713  Taniguchi H, Yamamoto H, Hirata T, Miyamoto N, Oki M, Nosho K, Adachi Y, Endo T, Imai K, Shinomura Y.  Frequent epigenetic inactivation of Wnt inhibitory factor-1 in human gastrointestinal cancers.  Oncogene  2005  24(53):7946-52  PubMed ID: 16007117
11007  Tetsuji Tanaka , Tao Bai, Kazunori Yukawa, Naohiko Umesaki  Radiation-induced cell death is independent of the apoptotic signals mediated by death-associated protein kinase in human cervical squamous cell carcinoma cells  Oncol Rep  2005  14(4):949-55  PubMed ID: 16142356
13825  Tanaka T.  Effects of anti-endometriotic therapies on Fas-mediated endometrial epithelial apoptosis  Oncol Rep  2005  13(6):1235-9  PubMed ID: 15870948
13795  Sugawara T, Nomura E, Fujimoto S.  Expression of enzyme associated with steroid hormone synthesis and local production of steroid hormone in endometrial carcinoma cells  J Endocrinol  2004  180(1):135-44  PubMed ID: 14709152
13800  Enomoto M, Seong JY, Kawashima S, Park MK.  Proliferation of TSU-Pr1, a human prostatic carcinoma cell line is stimulated by gonadotropin-releasing hormone  Life Sci . 2004 May 7;74(25):3141-52  2004  74(25):3141-52  PubMed ID: 15081579
13826  Bai T, Tanaka T, Yukawa K, Maeda M, Umesaki N.  Reduced expression of death-associated protein kinase in human uterine and ovarian carcinoma cells  Oncol Rep  2004  11(3):661-5.  PubMed ID: 14767518
14020  Tanaka T, Toujima S, Umesaki N.  Activin A inhibits growth-inhibitory signals by TGF-beta1 in differentiated human endometrial adenocarcinoma cells  Oncol Rep  2004  11(4):875-9  PubMed ID: 15010888
14021  Tanaka T, Toujima S, Umesaki N.  Growth-inhibitory signals by activin A do not affect anticancer drug-sensitivity and acquired multi-drug-resistance in human ovarian endometrioid adenocarcinoma OVK-18 cells  Oncol Rep  2004  11(3):667-71  PubMed ID: 14767519
13831  Unoki M, Nakamura Y.  Methylation at CpG islands in intron 1 of EGR2 confers enhancer-like activity  FEBS Lett  2003  554(1-2):67-72  PubMed ID: 14596916
13832  Tanaka T, Umesaki N.  Fas antigen (CD95) mediates cell survival signals to regulate functional cellular subpopulations in normal human endometrial stromal cells  Int J Mol Med  2003  11(6):757-62  PubMed ID: 12736718
14019  Tanaka T, Toujima S, Otani T, Minami S, Yamoto M, Umesaki N.  Expression and function of activin receptors in human endometrial adenocarcinoma cells  Int J Oncol  2003  23(3):657-63  PubMed ID: 12888901
2758  Harada, Hideki, Saijo, Kaoru, Watanabe, Satoru, Tsuboi, Koji, Nose, Tadao, Ishiwata, Isamu, Ohno, Tadao  Selective expansion of human natural killer cells from peripheral blood mononuclear cells by the cell line, HFWT.  Jpn J Cancer Res  2002  93:313-9  PubMed ID: 11927014
13797  Enomoto M, Mori T, Park M.  Influence of serum supplements in culture medium on gonadotropin-releasing hormone effects on colony formation  Life Sci  2002  71(18):2153-60  PubMed ID: 12204773
3101  Matsushima-Nishiu M, Unoki M, Ono K, Tsunoda T, Minaguchi T, Kuramoto H, Nishida M, Satoh T, Tanaka T, Nakamura Y  Growth and gene expression profile analyses of endometrial cancer cells expressing exogenous PTEN.  Cancer Res  2001  61:3741-9  PubMed ID: 11325847
13816  Enomoto M, Mori T, Park MK.  GnRH agonist Buserelin affects colony-forming efficiency of HHUA and Jurkat cells  Biochem Biophys Res Commun  2001  289(5):1180-7  PubMed ID: 11741317
13792  Tanaka T, Umesaki N.  Cytokine regulation of apoptotic susceptibility in a human endometrial epithelial cell line  J Reprod Immunol  2000  47(2):105-19  PubMed ID: 10924745
13819  Tauchi H, Komatsu K, Ishizaki K, Yatagai F, Kato T.  Mutation spectrum of MSH3-deficient HHUA/chr.2 cells reflects in vivo activity of the MSH3 gene product in mismatch repair  Mutat Res  2000  447(2):155-64  PubMed ID: 10751599
13824  Tanaka T, Mizuno K, Miyama M, Chang L, Chen H, Ohtaki S, Umesaki N, Ogita S.  Enhanced Fas/CD95-mediated apoptosis by epidermal growth factor in human endometrial epithelial cells  Eur J Obstet Gynecol Reprod Biol  1999  86(2):189-94  PubMed ID: 10509790
14015  Kunishige I, Samejima Y, Shiki Y, Moriyama A, Meruelo D, Saji F, Murata Y.  Suicide gene therapy for human uterine adenocarcinoma cells using herpes simplex virus thymidine kinase  Gynecol Oncol  1999  72(1):16-25  PubMed ID: 9889024
13791  Chang L, Tanaka T, Umesaki N, Ogita S.  Differential apoptotic susceptibility to anti-Fas IgM and anticancer drugs in a human endometrial adenocarcinoma cell line HHUA on laminin and type I collagen  Osaka City Med J  1998  44(2):173-80  PubMed ID: 10097597
13817  Tanaka T, Umesaki N, Mizuno K, Chang L, Ohtaki S, Ogita S.  Enhancement of apoptotic susceptibility by interleukin-1 beta in human endometrial epithelial cells  Gynecol Endocrinol  1998  12(5):315-9  PubMed ID: 9859023
13788  Kajitani K, Honda K, Terada H, Yasui T, Sumi T, Koyama M, Ishiko O.  Human Papillomavirus E6 Knockdown Restores Adenovirus Mediated-estrogen Response Element Linked p53 Gene Transfer in HeLa Cells  Asian Pac J Cancer Prev  2015  16(18):8239-45  PubMed ID: 26745067
13820  Mochizuki T, Sakai K, Iwashita M.  Effects of insulin-like growth factor (IGF) binding protein-3 (IGFBP-3) on endometrial cancer (HHUA) cell apoptosis and EGF stimulated cell proliferation in vitro  Growth Horm IGF Res  2006  16(3):202-10  PubMed ID: 16844395

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