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Cell No. : Cell Name
RCB0658 : HHUA  update : 2020/09/24
CommentExpressing receptor to estrogen and progesterone. Cell growth is slow.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, Isamu
Year of deposit 1991
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 54 years
Tissue endometrium
Case history Endometrial adenocarcinoma
Classification cancer
Year of origin 1980
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 15% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/1-2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 40-92(50) : /44(2),45(7),46(29),47(6),53(1),92(2)
deposit info
lot info
Reference information Reference 2
User's Publication 16

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1521  Ishiwata I, Ishiwata C, Ishikawa H  Effects of estradiol-17 beta and progesterone on cell proliferation and differentiation of the human endometrial carcinoma cell line (HHUA) in vitro.  Asia Oceania J Obstet Gynaecol  1984  10:531-8  PubMed ID: 6532401
1519  Ishiwata I, Ishiwata C, Soma M, Arai J, Ishikawa H  Establishment of human endometrial adenocarcinoma cell line containing estradiol-17 beta and progesterone receptors.  Gynecol Oncol  1984  17:281-90  PubMed ID: 6706228

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User's Publication
10248  Osanai K, Kobayashi Y, Otsu M, Izawa T, Sakai K, Iwashita M.  Ramelteon, a selective MT1/MT2 receptor agonist, suppresses the proliferation and invasiveness of endometrial cancer cells.  Hum. Cell  2017  30:209-215  PubMed ID: 28382516
10315  Ando H, Miyamoto T, Kashima H, Higuchi S, Ida K, Mvunta DH, Shiozawa T.  Panobinostat Enhances Growth Suppressive Effects of Progestin on Endometrial Carcinoma by Increasing Progesterone Receptor and Mitogen-Inducible Gene-6.  Horm Cancer  2017  8:257-267  PubMed ID: 28516379
9667  Chuwa AH, Sone K, Oda K, Ikeda Y, Fukuda T, Wada-Hiraike O, Inaba K, Makii C, Takeuchi M, Oki S, Miyasaka A, Kashiyama T, Arimoto T, Kuramoto H, Kawana K, Yano T, Osuga Y, Fujii T.  Significance of survivin as a prognostic factor and a therapeutic target in endometrial cancer.  Gynecol. Oncol.  2016  141:564-569  PubMed ID: 27079211
9687  Miyamoto T, Kashima H, Yamada Y, Kobara H, Asaka R, Ando H, Higuchi S, Ida K, Mvunta DH, Shiozawa T.  Lipocalin 2 Enhances Migration and Resistance against Cisplatin in Endometrial Carcinoma Cells.  PLoS ONE  2016  11:e0155220  PubMed ID: 27168162
7200  Asanoma K, Liu G, Yamane T, Miyanari Y, Takao T, Yagi H, Ohgami T, Ichinoe A, Sonoda K, Wake N, Kato K.  Regulation of the Mechanism of TWIST1 Transcription by BHLHE40 and BHLHE41 in Cancer Cells.  Mol. Cell. Biol.  2015  35:4096-109  PubMed ID: 26391953
7212  Asaka R, Miyamoto T, Yamada Y, Ando H, Mvunta DH, Kobara H, Shiozawa T.  Sirtuin 1 promotes the growth and cisplatin resistance of endometrial carcinoma cells: a novel therapeutic target.  Lab. Invest.  2015  95:1363-73  PubMed ID: 26367491
6463  Li H, Narahara H.  15-Deoxy-Δ(12,14)-prostaglandin J(2) induces growth inhibition, cell cycle arrest and apoptosis in human endometrial cancer cell lines.  Int J Mol Med.  2013  31(4):778-88  PubMed ID: 23403520
7588  Mitsuhashi Y, Horiuchi A, Miyamoto T, Kashima H, Suzuki A, Shiozawa T.  Prognostic significance of Notch signalling molecules and their involvement in the invasiveness of endometrial carcinoma cells.  Histopathology  2012  60:826-37  PubMed ID: 22348356
7708  Dong P, Kaneuchi M, Watari H, Hamada J, Sudo S, Ju J, Sakuragi N.  MicroRNA-194 inhibits epithelial to mesenchymal transition of endometrial cancer cells by targeting oncogene BMI-1.  Mol. Cancer  2011  10:99  PubMed ID: 21851624
7925  Miyamoto T, Kashima H, Suzuki A, Kikuchi N, Konishi I, Seki N, Shiozawa T.  Laser-captured microdissection-microarray analysis of the genes involved in endometrial carcinogenesis: stepwise up-regulation of lipocalin2 expression in normal and neoplastic endometria and its functional relevance.  Hum. Pathol.  2011  42:1265-74  PubMed ID: 21334721
8350  Fakhry H, Miyamoto T, Kashima H, Suzuki A, Ke H, Konishi I, Shiozawa T.  Immunohistochemical detection of histone deacetylases in endometrial carcinoma: involvement of histone deacetylase 2 in the proliferation of endometrial carcinoma cells.  Hum. Pathol.  2010  41:848-58  PubMed ID: 20178884
8539  Suzuki A, Horiuchi A, Ashida T, Miyamoto T, Kashima H, Nikaido T, Konishi I, Shiozawa T.  Cyclin A2 confers cisplatin resistance to endometrial carcinoma cells via up-regulation of an Akt-binding protein, periplakin.  J. Cell. Mol. Med.  2009  14:2305-17  PubMed ID: 19583808
8749  Matsumoto M, Yamaguchi Y, Seino Y, Hatakeyama A, Takei H, Niikura H, Ito K, Suzuki T, Sasano H, Yaegashi N, Hayashi S.  Estrogen signaling ability in human endometrial cancer through the cancer-stromal interaction.  Endocr. Relat. Cancer  2008  15:451-63  PubMed ID: 18508998
8772  Sugimoto T, Koizumi T, Sudo T, Yamaguchi S, Kojima A, Kumagai S, Nishimura R.  Correlative expression of cyclooxygenase-1 (Cox-1) and human epidermal growth factor receptor type-2 (Her-2) in endometrial cancer.  Kobe J Med Sci  2008  53:177-87  PubMed ID: 18204294
2758  Harada, Hideki, Saijo, Kaoru, Watanabe, Satoru, Tsuboi, Koji, Nose, Tadao, Ishiwata, Isamu, Ohno, Tadao  Selective expansion of human natural killer cells from peripheral blood mononuclear cells by the cell line, HFWT.  Jpn J Cancer Res  2002  93:313-9  PubMed ID: 11927014
3101  Matsushima-Nishiu M, Unoki M, Ono K, Tsunoda T, Minaguchi T, Kuramoto H, Nishida M, Satoh T, Tanaka T, Nakamura Y  Growth and gene expression profile analyses of endometrial cancer cells expressing exogenous PTEN.  Cancer Res  2001  61:3741-9  PubMed ID: 11325847

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