Cell No. : Cell Name RCB0657 : HOKUG  update : 2023/01/25
Comment			Human cell line derived from uterine cervix cancer. Glassy cell carcinoma.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Ishiwata, Isamu
		Originator	Ishiwata, Isamu
		Year of deposit	1991
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Female
		Age at sampling	53 years
		Tissue	cervix
		Disease name	glassy cell carcinoma
		Classification	cancer
		Year of origin	1986
		Lifespan	infinite
		Morphology	epithelial-like
		Cellosaurus(Expasy)	CVCL_8705
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		HamF12 + 15% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Virus (HIV)		(-)
		Isozyme		LD, NP
		Chromosome mode		39-85(50) : /41(7),42(22),43(4),81(2),82(1),83(5)/
		STR(human)		OK
Reference information		Reference	3
		User's Publication	3

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Reference

2568  Ishiwata I, Yoshikawa H, Nakagawa S, Kiguchi K, Ishikawa H  Human papillomavirus types 56 and 59 in novel cervical cancer cell lines.  Hum Cell  2000  13:55-6  PubMed ID: 10937347

2288  Oki A, Nishida M, Satoh T, Tsunoda H, Kasahara K, Saijo K, Kubo T, Ohno T  A novel human glassy-cell carcinoma cell line producing IL-6 and IL-8 from uterine cervix.  In Vitro Cell Dev Biol Anim  1998  34:290-7  PubMed ID: 9590502   DOI: 10.1007/s11626-998-0005-1

1520  Ishiwata I, Ishiwata C, Soma M, Ono I, Nakaguchi T, Nozawa S, Ishikawa H  Differences between cell lines of uterine cervical glassy cell carcinoma and large cell nonkeratinizing squamous cell carcinoma.  Anal Quant Cytol Histol  1990  12:290-8  PubMed ID: 1698374

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User's Publication

6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x

11007  Tetsuji Tanaka , Tao Bai, Kazunori Yukawa, Naohiko Umesaki  Radiation-induced cell death is independent of the apoptotic signals mediated by death-associated protein kinase in human cervical squamous cell carcinoma cells  Oncol Rep  2005  14(4):949-55  PubMed ID: 16142356

13826  Bai T, Tanaka T, Yukawa K, Maeda M, Umesaki N.  Reduced expression of death-associated protein kinase in human uterine and ovarian carcinoma cells  Oncol Rep  2004  11(3):661-5.  PubMed ID: 14767518