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Cell No. : Cell Name
RCB0657 : HOKUG  update : 2023/01/25
CommentHuman cell line derived from uterine cervix cancer. Glassy cell carcinoma.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, Isamu
Year of deposit 1991
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 53 years
Tissue cervix
Disease name glassy cell carcinoma
Classification cancer
Year of origin 1986
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_8705
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF12 + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 39-85(50) : /41(7),42(22),43(4),81(2),82(1),83(5)/
STR(human) OK
Reference information Reference 3
User's Publication 3

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2568  Ishiwata I, Yoshikawa H, Nakagawa S, Kiguchi K, Ishikawa H  Human papillomavirus types 56 and 59 in novel cervical cancer cell lines.  Hum Cell  2000  13:55-6  PubMed ID: 10937347  
2288  Oki A, Nishida M, Satoh T, Tsunoda H, Kasahara K, Saijo K, Kubo T, Ohno T  A novel human glassy-cell carcinoma cell line producing IL-6 and IL-8 from uterine cervix.  In Vitro Cell Dev Biol Anim  1998  34:290-7  PubMed ID: 9590502   DOI: 10.1007/s11626-998-0005-1
1520  Ishiwata I, Ishiwata C, Soma M, Ono I, Nakaguchi T, Nozawa S, Ishikawa H  Differences between cell lines of uterine cervical glassy cell carcinoma and large cell nonkeratinizing squamous cell carcinoma.  Anal Quant Cytol Histol  1990  12:290-8  PubMed ID: 1698374  

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User's Publication
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x
11007  Tetsuji Tanaka , Tao Bai, Kazunori Yukawa, Naohiko Umesaki  Radiation-induced cell death is independent of the apoptotic signals mediated by death-associated protein kinase in human cervical squamous cell carcinoma cells  Oncol Rep  2005  14(4):949-55  PubMed ID: 16142356  
13826  Bai T, Tanaka T, Yukawa K, Maeda M, Umesaki N.  Reduced expression of death-associated protein kinase in human uterine and ovarian carcinoma cells  Oncol Rep  2004  11(3):661-5.  PubMed ID: 14767518  

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