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Cell No. : Cell Name
RCB0649 : D52  update : 2024/01/17
CommentMoAb IgM to sea urchin sperm dynein IC1.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
National Institutes of Natural Sciences

5-1, Higashiyama, Myodaiji, Okazaki-Shi, Aichi-Ken, 444-8787, Japan
Dr.OGAWS Kazuo
Fax. +81-564-59-5191


English
Address
National Institutes of Natural Sciences

5-1, Higashiyama, Myodaiji, Okazaki-Shi, Aichi-Ken, 444-8787, Japan
Dr.OGAWS Kazuo
Fax. +81-564-59-5191
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ogawa, Kazuo
Originator Ogawa, Kazuo
Year of deposit 1991
Animal _mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U818
hybridoma Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_1 gender Female
Fusion_cell_2 name P3U1
Fusion_cell_2 strain mouse
Fusion method PEG, HAT selection
Antibody name anti-IC1 antibody
Subclass IgM
Immunogen 21S dynein of sea urchin sperm
Antigen_name intermediate chain (IC1)
Antigen_m.w. 115kDa
Antigen_character Flagellar outer arm dynein protein
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4-10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Antibody subclass IgM
Reference information Reference 1
User's Publication 0


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Reference
1885  Ogawa, K., Yokota, E., Hamada, Y., Wada, S., Okuno, M., Nakajima, Y.  The outer arm dynein α‐heavy chains of sea urchin sperm flagella and embryonic cilia are different  Cell Motil Cytoskeleton  1990  16:58-67    DOI: 10.1002/cm.970160108

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