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Cell No. : Cell Name
RCB0646 : D264  update : 2018/12/04
CommentMoAb IgM to sea urchin sperm dynein beta-heavy chain.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
National Institutes of Natural Sciences

5-1, Higashiyama, Myodaiji, Okazaki-Shi, Aichi-Ken, 444-8787, Japan
Dr.Ogawa, Kazuo
Fax. +81-564-59-5191
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Ogawa, Kazuo
Originator Ogawa, Kazuo
Year of deposit 1991
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
hybridoma Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name P3U1
Fusion_cell_2 strain mouse
Fusion method PEG, HAT selection
Antibody name anti-dynein β-heavy chain antibody
Subclass IgM
Immunogen 21S dynein of sea urchin sperm
Antigen_name dynein β-heavy chain
Antigen_m.w. 〜520kDa
Antigen_character lagellar and ciliary outer arm dyneins protein
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgM
Reference information Reference 1
User's Publication 0


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Reference
1885  Ogawa, K., Yokota, E., Hamada, Y., Wada, S., Okuno, M., Nakajima, Y.  The outer arm dynein α‐heavy chains of sea urchin sperm flagella and embryonic cilia are different  Cell Motil Cytoskeleton  1990  16:58-67 

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