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Cell No. : Cell Name
RCB0641 : EoL-1 cell  update : 2024/01/17
CommentEosinophilic leukemia. Differenciate by n-butylate treatment.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Saito, Hiroshi
Originator Saito, Hiroshi
Year of deposit 1991
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 33 years
Tissue blood
Disease name eosinophilic leukemia
Classification cancer
Year of origin 1988
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0258
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 49-52(50) : 49(1),50(13),51(33),52(3)
STR(human) OK
deposit info
lot info
Reference information Reference 5
User's Publication 46

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4787  Saito H, Hayakawa T, Mita H, Akiyama K, Shida T.  Effect of butyric acid on induction of differentiation into eosinophil-like cells in human eosinophilic leukemia cells, EoL-1 cell line: possible role of granulocyte-macrophage colony-stimulating factor as an autocrine differentiating factor.  Int Arch Allergy Immunol  1993  100(3):240-7  PubMed ID: 8453311   DOI: 10.1159/000236418
4788  Ohtsu H, Yamauchi K, Yoshie O, Tanno Y, Saito H, Hayashi N, Takishima T.  The effect of cytokines on the differentiation of an eosinophilic leukemia cell line (EoL-1) is associated with down regulation of c-myc gene expression.  Cell Struct Funct  1993  18(2):125-33  PubMed ID: 8364980   DOI: 10.1247/csf.18.125
4786  Morita M, Saito H, Honjo T, Saito Y, Tsuruta S, Kim KM, Tanaka M, Mori KJ, Mayumi M, Mikawa H.  Differentiation of a human eosinophilic leukemia cell line (EoL-1) by a human T-cell leukemia cell line (HIL-3)-derived factor.  Blood  1991  77(8):1766-75  PubMed ID: 1707698  
930  Yoshie O, Majima T, Saito H.  Membrane oxidative metabolism of human eosinophilic cell line EoL-1 in response to phorbol diester and formyl peptide: synergistic augmentation by interferon-gamma and tumor necrosis factor  J Leukoc Biol  1989  45(1):10-20  PubMed ID: 2536064   DOI: 10.1002/jlb.45.1.10
881  Saito H, Bourinbaiar A, Ginsburg M, Minato K, Ceresi E, Yamada K, Machover D, Breard J, Mathe G  Establishment and characterization of a new human eosinophilic leukemia cell line.  Blood  1985  66:1233-40  PubMed ID: 2415185  

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User's Publication
17526  Chueahongthong F, Tima S, Chiampanichayakul S, Dejkriengkraikul P, Okonogi S, Sasarom M, Rodwattanagul S, Berkland C, Anuchapreeda S.  Doxorubicin-Loaded Polymeric Micelles Conjugated with CKR- and EVQ-FLT3 Peptides for Cytotoxicity in Leukemic Stem Cells  Pharmaceutics  2022  14(10):2115  PubMed ID: 36297550   DOI: 10.3390/pharmaceutics14102115
20378  Shimizu S, Tojima I, Nakamura K, Arai H, Kouzaki H, Shimizu T.  Nasal polyp fibroblasts (NPFs)-derived exosomes are important for the release of vascular endothelial growth factor from cocultured eosinophils and NPFs.  Auris Nasus Larynx  2022    PubMed ID: 34736807   DOI: 10.1016/j.anl.2021.10.002
14476  Fah Chueahongthong, Singkome Tima, Sawitree Chiampanichayakul, Cory Berkland, Songyot Anuchapreeda  The effect of co-treatments of chemotherapeutic drugs and curcumin on cytotoxicity and FLT3 protein expression in leukemic stem cells  Research Square  2021      DOI: 10.21203/
16275  Chueahongthong F, Tima S, Chiampanichayakul S, Berkland C, Anuchapreeda S.  Co-Treatments of Edible Curcumin from Turmeric Rhizomes and Chemotherapeutic Drugs on Cytotoxicity and FLT3 Protein Expression in Leukemic Stem Cells  Molecules  2021  26(19):5785  PubMed ID: 34641328   DOI: 10.3390/molecules26195785
13042  Yoshihara A, Sekine R, Yamada Y, Takai M.  Study on polyethylene glycol cross-linker in peptide-conjugated antibody on efficiency of cell capture and release.  Anal Biochem  2020    PubMed ID: 32470345   DOI: 10.1016/j.ab.2020.113790
13358  Imoto Y, Takabayashi T, Sakashita M, Kato Y, Yoshida K, Kidoguchi M, Koyama K, Adachi N, Kimura Y, Ogi K, Ito Y, Kanno M, Okamoto M, Narita N, Fujieda S.  Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis.  Biomolecules  2020    PubMed ID: 33218117   DOI: 10.3390/biom10111568
14832  Saito S, Kitamura-Muramatsu Y, Komine F, Polat M, Takeshima SN, Takei M, Aida Y.  Absence of bovine leukemia virus proviral DNA in Japanese human blood cell lines and human cancer cell lines  Arch Virol  2020  165(1):207-214  PubMed ID: 31776677   DOI: 10.1007/s00705-019-04474-9
10885  Wuttichai Tata, Viraporn Thepbundit, Chatsri Kuansuwan, Kanya Preechasuth,  Effects of crude medicinal Thai flower extracts on cytotoxicity and FMS-like tyrosine kinase 3 protein expression in EoL-1 leukemic cell line  Journal of Associated Medical Sciences  2019  52(1): 1-7   
10674  Harada E, D'Alessandro-Gabazza CN, Toda M, Morizono T, Totoki T, Yasuma T, Nishihama K, Kobayashi T, Sumiya T, Kawagishi H, Gabazza EC.  The Medicinal Mushroom, Grifola gargal, Ameliorates Allergic Bronchial Asthma.  J Med Food  2018  21:136-145  PubMed ID: 29261008   DOI: 10.1089/jmf.2017.4016
4082  Kee-Hyung Sung, In-Sik Kim, and Ji-Sook Lee4  Different Protein Expression between Human Eosinophilic Leukemia Cells, EoL-1 and Imatinib-resistant EoL-1 Cells, EoL-1-IR  Biomed Sci Lett   2018  24:426-429    DOI: 10.15616/BSL.2018.24.4.426
4185  Pan B, Yang J, Wang X, Xu K, Ikezoe T.  miR-217 sensitizes chronic myelogenous leukemia cells to tyrosine kinase inhibitors by targeting pro-oncogenic anterior gradient 2.  Exp. Hematol.  2018    PubMed ID: 30195077   DOI: 10.1016/j.exphem.2018.09.001
10550  Lu H, Xie RD, Lin R, Zhang C, Xiao XJ, Li LJ, Liu ZQ, Yang LT, Feng BS, Liu ZJ, Yang PC.  Vitamin D-deficiency induces eosinophil spontaneous activation.  Cell. Immunol.  2017  322:56-63  PubMed ID: 29050663   DOI: 10.1016/j.cellimm.2017.10.003
10613  Lee YZ, Yap HM, Shaari K, Tham CL, Sulaiman MR, Israf DA.  Blockade of Eosinophil-Induced Bronchial Epithelial-Mesenchymal Transition with a Geranyl Acetophenone in a Coculture Model.  Front Pharmacol  2017  8:837  PubMed ID: 29201006   DOI: 10.3389/fphar.2017.00837
14371  Kainuma K, Kobayashi T, D'Alessandro-Gabazza CN, Toda M, Yasuma T, Nishihama K, Fujimoto H, Kuwabara Y, Hosoki K, Nagao M, Fujisawa T, Gabazza EC.  β 2 adrenergic agonist suppresses eosinophil-induced epithelial-to-mesenchymal transition of bronchial epithelial cells  Respir Res  2017  18(1):79  PubMed ID: 28464879   DOI: 10.1186/s12931-017-0563-4
14380  Shirasaki H, Kanaizumi E, Himi T.  Leukotriene D 4 induces chemotaxis in human eosinophilc cell line, EoL-1 cells via CysLT1 receptor activation  Heliyon  2017  3(11):e00464  PubMed ID: 29264420   DOI: 10.1016/j.heliyon.2017.e00464
12224  Singkome Tima, Siriporn Okonogi, Chadarat Ampasavate, Chad Pickens, Cory Berkland, Songyot Anuchapreeda  Development and Characterization of FLT3-Specific Curcumin-Loaded Polymeric Micelles as a Drug Delivery System for Treating FLT3-Overexpressing Leukemic Cells  J Pharm Sci  2016  105(12):3645-3657  PubMed ID: 27751588   DOI: 10.1016/j.xphs.2016.09.010
9127  Rungkarn Sangkaruk, Singkome Tima, Methee Rungrojsakul, Sawitree Chiampanichayakul, Songyot Anuchapreeda  Effects of Saraphi (Mammea siamensis) flower extracts on cell proliferation and Fms-like tyrosine kinase 3 expression in leukemic EoL-1 cell line  Journal of Associated Medical Sciences  2016  49    DOI: 10.14456/jams.2016.19
12413  Park, Kyeong Hun ; Lee, Eun Suk ; Jin, Yong Ik ; Myung, Kyung Sun ; Park, Hong Woo ; Park, Chun Geon ; Kong, Won Sik ; Kim, Young Ock  Inhibitory effect of Panax ginseng and Pleurotus osteratus complex on expression of cytokine genes induced by extract of Dermatophagoides pteronissinus in human monocytic THP-1 and EoL-1 cells  Journal of Mushroom  2016  14    DOI: 10.14480/JM.2016.14.4.155
6059  Takeuchi A, Nishioka C, Ikezoe T, Yang J, Yokoyama A.  STAT5A regulates DNMT3A in CD34(+)/CD38(-) AML cells.  Leuk Res  2015  39(8):897-905  PubMed ID: 26059451   DOI: 10.1016/j.leukres.2015.05.006
6061  Nishioka C, Ikezoe T, Yang J, Yokoyama A.  Tetraspanin Family Member, CD82, Regulates Expression of EZH2 via Inactivation of p38 MAPK Signaling in Leukemia Cells.  PLoS One  2015  10(5):e0125017  PubMed ID: 25955299   DOI: 10.1371/journal.pone.0125017
14996  Roeen Z, Toda M, D'Alessandro-Gabazza CN, Onishi M, Kobayashi T, Yasuma T, Urawa M, Taguchi O, Gabazza EC.  Thrombomodulin inhibits the activation of eosinophils and mast cells  Cell Immunol  2015  293(1):34-40  PubMed ID: 25497974   DOI: 10.1016/j.cellimm.2014.11.005
5834  Liu WN, Leung KN.  Apoptosis- and differentiation-inducing activities of jacaric acid, a conjugated linolenic acid isomer, on human eosinophilic leukemia EoL-1 cells.  Oncol Rep  2014  32(5):1881-8  PubMed ID: 25174702   DOI: 10.3892/or.2014.3446
10321  In-Hwan Baek, Byung-Yo Lee, Jung-Woo Chae, Gyu Yong Song, Wonku Kang, Kwang-Il Kwon  Development of a Pharmacokinetic/Pharmacodynamic/Disease Progression Model in NC/Nga Mice for Development of Novel Anti-Atopic Dermatitis Drugs  Xenobiotica  2014  44(11):975-87  PubMed ID: 24874886   DOI: 10.3109/00498254.2014.924058
6079  Nishioka C, Ikezoe T, Furihata M, Yang J, Serada S, Naka T, Nobumoto A, Kataoka S, Tsuda M, Udaka K, Yokoyama A.  CD34⁺/CD38⁻ acute myelogenous leukemia cells aberrantly express CD82 which regulates adhesion and survival of leukemia stem cells.  Int J Cancer  2013  132(9):2006-19  PubMed ID: 23055153   DOI: 10.1002/ijc.27904
16089  El-Shazly AE, Begon DY, Kustermans G, Arafa M, Dortu E, Henket M, Lefebvre PP, Louis R, Delvenne P.  Novel association between vasoactive intestinal peptide and CRTH2 receptor in recruiting eosinophils: a possible biochemical mechanism for allergic eosinophilic inflammation of the airways  J Biol Chem  2013  288(2):1374-84  PubMed ID: 23168411   DOI: 10.1074/jbc.M112.422675
16302  In Sik Kim, Dong-Hee Kim, Chi-Young Yun, Ji-Sook Lee  A (S)-(+)-decursin derivative, (S)-(+)-3-(3,4-dihydroxy-phenyl)-acrylic acid 2,2-dimethyl-8-oxo-3,4-dihydro-2H,8H-pyrano[3,2-g]-chromen-3-yl-ester, attenuates the development of atopic dermatitis-like lesions in NC/Nga mice  Molecular Biology Reports  2013  40:2541-2548  PubMed ID: 23292074   DOI: 10.1007/s11033-012-2339-8
6103  Yamamoto Y, Tsuzuki S, Akahori Y, Ukai Y, Sumitomo M, Murayama Y, Yamamoto K, Inaguma Y, Tokuda M, Abe A, Akatsuka Y, Emi N, Kurosawa Y.  Isolation of human mAbs that directly modulate FMS-related tyrosine kinase 3 signaling.  Cancer Sci  2012  103(2):350-9  PubMed ID: 22049994   DOI: 10.1111/j.1349-7006.2011.02141.x
7713  El-Shazly AE, Moonen V, Mawet M, Begon D, Henket M, Arafa M, Louis R, Delvenne P, Lefebvre PP.  IFN-γ and TNF-α potentiate prostaglandin D2-induced human eosinophil chemotaxis through up-regulation of CRTH2 surface receptor.  Int. Immunopharmacol.  2011  11:1864-70  PubMed ID: 21835268   DOI: 10.1016/j.intimp.2011.07.017
7782  Ogawa T, Shimizu S, Tojima I, Kouzaki H, Shimizu T.  Heparin inhibits mucus hypersecretion in airway epithelial cells.  Am J Rhinol Allergy  2011  25:69-74  PubMed ID: 21679503   DOI: 10.2500/ajra.2011.25.3562
7979  Koh DJ, Ahn HS, Chung HS, Lee H, Kim Y, Lee JY, Kim DG, Hong M, Shin M, Bae H.  Inhibitory effects of casticin on migration of eosinophil and expression of chemokines and adhesion molecules in A549 lung epithelial cells via NF-κB inactivation.  J Ethnopharmacol  2011  136:399-405  PubMed ID: 21251967   DOI: 10.1016/j.jep.2011.01.014
8198  Yang EJ, Lee JS, Yun CY, Ryang YS, Kim JB, Kim IS.  Suppression of ovalbumin-induced airway inflammatory responses in a mouse model of asthma by Mimosa pudica extract.  Phytother Res  2011  25:59-66  PubMed ID: 20623591   DOI: 10.1002/ptr.3220
15726  Nishioka C, Ikezoe T, Yang J, Udaka K, Yokoyama A.  Imatinib causes epigenetic alterations of PTEN gene via upregulation of DNA methyltransferases and polycomb group proteins  Blood Cancer J  2011  1(12):e4  PubMed ID: 22829096   DOI: 10.1038/bcj.2011.33
18258  Ikezoe T, Yang J, Nishioka C, Yokoyama A.  p53 is critical for the Aurora B kinase inhibitor-mediated apoptosis in acute myelogenous leukemia cells  Int J Hematol  2010  91(1):69-77  PubMed ID: 20013323   DOI: 10.1007/s12185-009-0462-7
8209  Nishioka C, Ikezoe T, Yang J, Yokoyama A.  Long-term exposure of leukemia cells to multi-targeted tyrosine kinase inhibitor induces activations of AKT, ERK and STAT5 signaling via epigenetic silencing of the PTEN gene.  Leukemia  2010  24:1631-40  PubMed ID: 20596030   DOI: 10.1038/leu.2010.145
8519  Lee JS, Yang EJ, Kim IS.  The roles of MCP-1 and protein kinase C delta activation in human eosinophilic leukemia EoL-1 cells.  Cytokine  2009  48:186-95  PubMed ID: 19664934   DOI: 10.1016/j.cyto.2009.07.008
8522  Zheng PY, Feng BS, Oluwole C, Struiksma S, Chen X, Li P, Tang SG, Yang PC.  Psychological stress induces eosinophils to produce corticotrophin releasing hormone in the intestine.  Gut  2009  58:1473-9  PubMed ID: 19651632   DOI: 10.1136/gut.2009.181701
8560  Sohn SH, Ko E, Oh BG, Kim J, Choi E, Kim SH, Kim Y, Shin M, Hong M, Bae H.  Global gene analysis of Erigeron canadensis-treated TNF-alpha-, IL-4- and IL-1beta-stimulated A549 human epithelial cells.  Ann. Nutr. Metab.  2009  54:227-35  PubMed ID: 19521071   DOI: 10.1159/000225378
8623  Oh BG, Lee H, Kim Y, Shin M, Hong M, Jung SK, Kim J, Bae H.  Inhibitory effects of Schizandrae Fructus on eotaxin secretion in A549 human epithelial cells and eosinophil migration.  Phytomedicine  2009  16:814-22  PubMed ID: 19324539   DOI: 10.1016/j.phymed.2009.01.009
6439  Miyazawa K, Kakazu N, Ohyashiki K.  Clinical features of hypereosinophilic syndrome: FIP1L1-PDGFRA fusion gene-positive disease is a distinct clinical entity with myeloproliferative features and a poor response to corticosteroid.  Int J Hematol.  2007  85(1):5-10  PubMed ID: 17261495   DOI: 10.1532/IJH97.06047
10368  Jung Mogg Kim 1, Joo Sung Kim, Jin Young Lee, Yeong-Jeon Kim, Ho-Joo Youn, In Young Kim, Young Joon Chee, Yu-Kyoung Oh, Nayoung Kim, Hyun Chae Jung, In Sung Song  Vacuolating Cytotoxin in Helicobacter Pylori Water-Soluble Proteins Upregulates Chemokine Expression in Human Eosinophils via Ca2+ Influx, Mitochondrial Reactive Oxygen Intermediates, and NF-kappaB Activation  Infect Immun  2007  75(7):3373-81  PubMed ID: 17452475   DOI: 10.1128/IAI.01940-06
2902  Terakawa M, Tomimori Y, Goto M, Hayashi Y, Oikawa S, Fukuda Y.  Eosinophil migration induced by mast cell chymase is mediated by extracellular signal-regulated kinase pathway.  Biochem Biophys Res Commun  2005  32(4):969-75  PubMed ID: 15919053   DOI: 10.1016/j.bbrc.2005.04.172
3360  Kodama, Norio, Kambayashi, Yasuhiro, Kubo, Masayuki, Nobukuni, Yoshitaka, Kimura, Shingo, Nakamura, Hiroyuki, Ogino, Keiki  Induction of myeloperoxidase and nitrotyrosine formation in a human eosinophilic leukemia cell line, EoL-1.  Cell Biochem Funct  2004  22:105-12  PubMed ID: 15027099   DOI: 10.1002/cbf.1051
3116  Imaizumi, Tadaatsu, Kumagai, Mika, Sasaki, Naoko, Kurotaki, Hidekachi, Mori, Fumiaki, Seki, Masako, Nishi, Nozomu, Fujimoto, Koji, Tanji, Kunikazu, Shibata, Takeo, Tamo, Wakako, Matsumiya, Tomoh, Yoshida, Hidemi, Cui, Xue-Fan, Takanashi, Shingo, Hanada, Katsumi, Okumura, Ken, Yagihashi, Soroku, Wakabayashi, Koichi, Nakamura, Takanori, Hirashima, Mitsuomi, Satoh, Kei  Interferon-gamma stimulates the expression of galectin-9 in cultured human endothelial cells.  J Leukoc Biol  2002  72:486-91  PubMed ID: 12223516  
6259  Kawahara H, Maeda-Yamamoto M, Suzuki M, Hakamata K.  New human fusion partners, ICLU-B, -T, -E for making human hybridomas derived from various immunological cells.  Hum Antibodies  1999  9(2):89-94  PubMed ID: 10405829  
12270  C K Wong, C Y Ho, C W Lam, J P Zhang, N M Hjelm  Differentiation of a human eosinophilic leukemic cell line, EoL-1: characterization by the expression of cytokine receptors, adhesion molecules, CD95 and eosinophilic cationic protein (ECP)  Immunol Lett  1999  68(2-3):317-23  PubMed ID: 10424438   DOI: 10.1016/s0165-2478(99)00064-4
2119  Yoshinobu Matsuo, Ayumi Okochi, Toshio Ariyasu, Emi Iimura and Tadao Ohno  Identification of Cell Lines with Variabel Numbers of Tandem Repeat(VNTR) Amplified by Polymerase Chain Reaction  Tiss Cult Res Commun  1996  15:211-219    DOI: 10.11418/jtca1981.15.4_21

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