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Cell No. : Cell Name
RCB0638 : TCS  update : 2020/09/24
CommentKeratinizing cervix squamous carcinoma, CEA(+)
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Izumi, Rikuichi
Originator Izumi, Rikuichi & Kawabata, Masakiyo
Year of deposit 1991
Another name TC-S
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 78 years
Tissue cervix, lymph node meta
Case history large cell, keratinizing
Metastastatic ability Yes
Metastatsd tissue lymph node
Classification cancer
Year of origin 1982
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 89-180(50) : /83(2),84(3),85(5),86(5),87(5),88(3)/
Reference information Reference 1
User's Publication 8


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Reference
1792  Kawabata M  New establishment and characterization of a carcinoembryonic antigen (CEA)-producing cell line from a human carcinoma of the uterine cervix.  Nippon Sanka Fujinka Gakkai Zasshi  1984  36:2619-28  PubMed ID: 6520483

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User's Publication
7659  Murata T, Mizushima H, Chinen I, Moribe H, Yagi S, Hoffman RM, Kimura T, Yoshino K, Ueda Y, Enomoto T, Mekada E.  HB-EGF and PDGF mediate reciprocal interactions of carcinoma cells with cancer-associated fibroblasts to support progression of uterine cervical cancers.  Cancer Res.  2011  71:6633-42  PubMed ID: 22009535
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309
9059  Y-C Jung, E Mizuki, T Akao, J-C Côté  Isolation and Characterization of a Novel Bacillus Thuringiensis Strain Expressing a Novel Crystal Protein With Cytocidal Activity Against Human Cancer Cells  J Appl Microbiol  2007  103 (1), 65-79  PubMed ID: 17584453
3335  Yoshino K, Iimura E, Saijo K, Iwase S, Fukami K, Ohno T, Obata Y, Nakamura Y.  Essential role for gene profiling analysis in the authentication of human cell lines.  Hum Cell  2006  19(1):43-8  PubMed ID: 16643607
3413  Yamashita, Satoko, Katayama, Hideki, Saitoh, Hiroyuki, Akao, Tetsuyuki, Park, Yu Shin, Mizuki, Eiichi, Ohba, Michio, Ito, Akio  Typical Three-Domain Cry Proteins of Bacillus thuringiensis Strain A1462 Exhibit Cytocidal Activity on Limited Human Cancer Cells.  J Biochem  2005  138:663-72  PubMed ID: 16428294
3213  Kanao, Hiroyuki, Enomoto, Takayuki, Kimura, Toshihiro, Fujita, Masami, Nakashima, Ryuichi, Ueda, Yutaka, Ueno, Yuko, Miyatake, Takashi, Yoshizaki, Tatsuo, Buzard, Gregory S, Tanigami, Akira, Yoshino, Kiyoshi, Murata, Yuji  Overexpression of LAMP3/TSC403/DC-LAMP promotes metastasis in uterine cervical cancer.  Cancer Res  2005  65:8640-5  PubMed ID: 16204031
9048  Shiro Okumura, Hiroyuki Saitoh, Tomoyuki Ishikawa, Naoya Wasano, Satoko Yamashita, Ken-Ichi Kusumoto, Tetsuyuki Akao, Eiichi Mizuki, Michio Ohba, Kuniyo Inouye  Identification of a Novel Cytotoxic Protein, Cry45Aa, From Bacillus Thuringiensis A1470 and Its Selective Cytotoxic Activity Against Various Mammalian Cell Lines  J Agric Food Chem  2005  53 (16), 6313-8  PubMed ID: 16076112
2894  Ito, Akio, Sasaguri, Yasuyuki, Kitada, Sakae, Kusaka, Yoshitomo, Kuwano, Kyoko, Masutomi, Kenjiro, Mizuki, Eiichi, Akao, Tetsuyuki, Ohba, Michio  A Bacillus thuringiensis crystal protein with selective cytocidal action to human cells.  J Biol Chem  2004  279:21282-6  PubMed ID: 15026424



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