Cell No. : Cell Name RCB0637 : CTLL-2  update : 2022/03/22
Comment			Cytotoxic T-cell line. IL-2 dependent.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Todokoro, Kazuo
		Originator	Smith, K. A.
		Year of deposit	1991
		Animal	mouse < Mammals
		Tissue	T cell
		Classification	transformed
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_0227
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Medium and additives		RPMI1640 + 10% FBS + 50-100U/ml mouse IL-2
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 10 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		SSLP(mouse)		OK
		Isozyme		LD, NP
Images			deposit info	lot info
Reference information		Reference	5
		User's Publication	16

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Reference

2614  Fernandez-Botran R, Sanders, V M, Mosmann, T R, Vitetta, E S  Lymphokine-mediated regulation of the proliferative response of clones of T helper 1 and T helper 2 cells.  J Exp Med  1988  168:543-58  PubMed ID: 2970518   DOI: 10.1084/jem.168.2.543

4784  Gillis S, Scheid M, Watson J.  Biochemical and biologic characterization of lymphocyte regulatory molecules. III. The isolation and phenotypic characterization of Interleukin-2 producing T cell lymphomas.  J Immunol  1980  125(6):2570-8  PubMed ID: 6968790

4785  Mochizuki D, Watson J, Gillis S.  Biochemical and biologic characterization of lymphocyte regulatory molecules. IV. Purification of Interleukin 2 from a murine T cell lymphoma.  J Immunol  1980  125(6):2579-83  PubMed ID: 7000903

2613  Gillis S, Smith, K A  In vitro generation of tumor-specific cytotoxic lymphocytes. Secondary allogeneic mixed tumor lymphocyte culture of normal murine spleen cells.  J Exp Med  1977  146:468-82  PubMed ID: 69003   DOI: 10.1084/jem.146.2.468

1370  Gillis S, Smith, K A  Long term culture of tumour-specific cytotoxic T cells.  Nature  1977  268:154-6  PubMed ID: 145543   DOI: 10.1038/268154a0

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User's Publication

20352  Takenaga K, Koshikawa N, Nagase H.  Intercellular transfer of mitochondrial DNA carrying metastasis-enhancing pathogenic mutations from high- to low-metastatic tumor cells and stromal cells via extracellular vesicles.  BMC Mol Cell Biol  2021  22(1):52  PubMed ID: 34615464   DOI: 10.1186/s12860-021-00391-5

16127  Kobayashi M, Kojima K, Murayama K, Amano Y, Koyama T, Ogama N, Takeshita T, Fukuhara T, Tanaka N.  MK-6, a novel not-α IL-2, elicits a potent antitumor activity by improving the effector to regulatory T cell balance  Cancer Sci   2021  112(11):4478-4489  PubMed ID: 34545658   DOI: 10.1111/cas.15127

12411  Endo K, Kito H, Tanaka R, Kajikuri J, Tanaka S, Elboray EE, Suzuki T, Ohya S.  Possible Contribution of Inflammation-Associated Hypoxia to Increased K 2P 5.1 K + Channel Expression in CD4 + T cells of the Mouse Model for Inflammatory Bowel Disease  J Mol Sci  2019  21(1):38  PubMed ID: 31861667   DOI: 10.3390/ijms21010038

7294  Asama T, Arima TH, Gomi T, Keishi T, Tani H, Kimura Y, Tatefuji T, Hashimoto K.  Lactobacillus kunkeei YB38 from honeybee products enhances IgA production in healthy adults.  J. Appl. Microbiol.  2015  119:818-26  PubMed ID: 26121394   DOI: 10.1111/jam.12889

5334  Mimoto F, Katada H, Kadono S, Igawa T, Kuramochi T, Muraoka M, Wada Y, Haraya K, Miyazaki T, Hattori K.  Engineered antibody Fc variant with selectively enhanced FcγRIIb binding over both FcγRIIa(R131) and FcγRIIa(H131).  Protein Eng Des Sel  2013  26(10):589-98  PubMed ID: 23744091   DOI: 10.1093/protein/gzt022

8048  Kobayashi N, Saito T, Uematsu T, Kishi K, Toba M, Kohda N, Suzuki T.  Oral administration of heat-killed Lactobacillus pentosus strain b240 augments protection against influenza virus infection in mice.  Int. Immunopharmacol.  2011  11:199-203  PubMed ID: 21095259   DOI: 10.1016/j.intimp.2010.11.019

15597  Usui T, Nagumo Y, Watanabe A, Kubota T, Komatsu K, Kobayashi J, Osada H.  Brasilicardin A, a natural immunosuppressant, targets amino Acid transport system L  Chem Biol   2006  13(11):1153-60  PubMed ID: 17113997   DOI: 10.1016/j.chembiol.2006.09.006

2705  Kushida S, Peng, B G, Uchimura E, Kuang M, Huang L, Miwa M, Ohno T  A tumour vaccine of fixed tumour fragments in a controlled-release vehicle with cytokines for therapy of hepatoma in mice.  Dig Liver Dis  2004  36:478-85  PubMed ID: 15285528   DOI: 10.1016/j.dld.2004.02.010

2765  Shou, Zhangfei, Yamada, Kazuya, Kawata, Hiroko, Yokoyama, Osamu, Miyamoto, Kaoru  A mechanism of induction of the mouse zinc-fingers and homeoboxes 1 (ZHX1) gene expression by interleukin-2.  Biochem Biophys Res Commun  2004  314:885-90  PubMed ID: 14741719   DOI: 10.1016/j.bbrc.2003.12.162

2474  Fukushima K, Yamashita K  Interleukin-2 carbohydrate recognition modulates CTLL-2 cell proliferation.  J Biol Chem  2001  276:7351-6  PubMed ID: 11076950   DOI: 10.1074/jbc.M008781200

2560  Ito A, Kataoka, T R, Kim, D K, Koma Y, Lee, Y M, Kitamura Y  Inhibitory effect on natural killer activity of microphthalmia transcription factor encoded by the mutant mi allele of mice.  Blood  2001  97:2075-83  PubMed ID: 11264174   DOI: 10.1182/blood.v97.7.2075

4871  Feng R, Kabayama A, Uchida K, Hoshino H, Miwa M.  Cell-free entry of human T-cell leukemia virus type 1 to mouse cells.  Jpn J Cancer Res  2001  92(4):410-6  PubMed ID: 11346463   DOI: 10.1111/j.1349-7006.2001.tb01110.x

5333  Fukushima K, Hara-Kuge S, Ideo H, Yamashita K.  Carbohydrate recognition site of interleukin-2 in relation to cell proliferation.  J Biol Chem  2001  276(33):31202-8  PubMed ID: 11390392   DOI: 10.1074/jbc.M102789200

11035  T Matsuguchi, K Takagi, T Musikacharoen, Y Yoshikai  Gene expressions of lipopolysaccharide receptors, toll-like receptors 2 and 4, are differently regulated in mouse T lymphocytes  Blood  2000  95(4):1378-85.  PubMed ID: 10666214

7040  Yu F, Itoyama Y, Kira J, Fujihara K, Kobayashi T, Kitamoto T, Suzumura A, Yamamoto N, Nakajima Y, Goto I.  TNF-beta produced by human T lymphotropic virus type I-infected cells influences the proliferation of human endothelial cells and fibroblasts.  J Immunol  1994  152(12):5930-8  PubMed ID: 8207218

1773  Xu, B., Aoyama, K. and Ueda, A.  In Vitro Evaluation of Contact Sensitivity to DNCB Using Hapten-Stimulatied In Vitro Interleukin-2 Production by Murine Lymph Node Cells  Toxicology Methods  1993  3:183-194    DOI: 10.3109/15376519309044575