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Cell No. : Cell Name
RCB0637 : CTLL-2  update : 2024/01/17
CommentCytotoxic T-cell line. IL-2 dependent.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Todokoro, Kazuo
Originator Smith, K. A.
Year of deposit 1991
Animal _mouse < Mammals
Tissue T cell
Classification transformed
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0227
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS + 50-100U/ml mouse IL-2
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 17
User's Publication 16

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922  Almeida JL, Dakic A, Kindig K, Kone M, Letham DLD, Langdon S, Peat R, Holding-Pillai J, Hall EM, Ladd M, Shaffer MD, Berg H, Li J, Wigger G, Lund S, Steffen CR, Fransway BB, Geraghty B, Natoli M, Bauer B, Gollin SM, Lewis DW, Reid Y.  Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines  PLoS One  2019  14(6):e0218412  PubMed ID: 31220119   DOI: 10.1371/journal.pone.0218412
878  Adams JE, Peña MT, Gillis TP, Williams DL, Adams LB, Truman RW.  Expression of nine-banded armadillo (Dasypus novemcinctus) interleukin-2 in E. coli  Cytokine  2005  32(5):219-25  PubMed ID: 16338142   DOI: 10.1016/j.cyto.2005.09.011
188  Cho YJ, Lee JS.  The effect of TGF-beta on the induction CD8 and NK1.1 expression in CTLL-2 cell line  J Investig Allergol Clin Immunol  2003  13(4):259-65  PubMed ID: 14989115  
879  Beadling C, Cereseto A, Fan W, Naramura M, Smith KA.  Cytokine response gene 8 (CR8) regulates the cell cycle G1-S phase transition and promotes cellular survival  Oncogene  2001  20(14):1771-83  PubMed ID: 11313924   DOI: 10.1038/sj.onc.1204212
214  Weston L, Geczy A, Farrell C.  A convenient and reliable IL-2 bioassay using frozen CTLL-2 to improve the detection of helper T lymphocyte precursors  Immunol Cell Biol  1998  76(2):190-2  PubMed ID: 9619491   DOI: 10.1046/j.1440-1711.1998.00733.x
215  Winandy M, Lewalle P, Deneys V, Ferrant A, De Bruyère M.  Improved IL-2 detection for determination of helper T lymphocyte precursor frequency in limiting dilution assay  J Immunol Methods  1998  215(1-2):81-94  PubMed ID: 9744750   DOI: 10.1016/s0022-1759(98)00065-9
148  Inge TH, McCoy KM, Susskind BM, Barrett SK, Zhao G, Bear HD.  Immunomodulatory effects of transforming growth factor-beta on T lymphocytes. Induction of CD8 expression in the CTLL-2 cell line and in normal thymocytes  J Immunol  1992  148(12):3847-56  PubMed ID: 1602133  
2614  Fernandez-Botran R, Sanders, V M, Mosmann, T R, Vitetta, E S  Lymphokine-mediated regulation of the proliferative response of clones of T helper 1 and T helper 2 cells.  J Exp Med  1988  168:543-58  PubMed ID: 2970518   DOI: 10.1084/jem.168.2.543
217  Gillis S, Gillis AE, Henney CS.  Monoclonal antibody directed against interleukin 2. I. Inhibition of T lymphocyte mitogenesis and the in vitro differentiation of alloreactive cytolytic T cells  J Exp Med  1981  154(3):983-8  PubMed ID: 6974222   DOI: 10.1084/jem.154.3.983
220  Kern DE, Gillis S, Okada M, Henney CS.  The role of interleukin-2 (IL-2) in the differentiatin of cytotoxic T cells: the effect of monoclonal anti-IL-2 antibody and absorption with IL-2 dependent T cell lines  J Immunol  1981  127(4):1323-8  PubMed ID: 6456304  
4784  Gillis S, Scheid M, Watson J.  Biochemical and biologic characterization of lymphocyte regulatory molecules. III. The isolation and phenotypic characterization of Interleukin-2 producing T cell lymphomas.  J Immunol  1980  125(6):2570-8  PubMed ID: 6968790  
4785  Mochizuki D, Watson J, Gillis S.  Biochemical and biologic characterization of lymphocyte regulatory molecules. IV. Purification of Interleukin 2 from a murine T cell lymphoma.  J Immunol  1980  125(6):2579-83  PubMed ID: 7000903  
216  Baker PE, Gillis S, Smith KA.  Monoclonal cytolytic T-cell lines  J Exp Med  1979  149(1):273-8  PubMed ID: 310861   DOI: 10.1084/jem.149.1.273
218  Gillis S, Ferm MM, Ou W, Smith KA.  T cell growth factor: parameters of production and a quantitative microassay for activity  J Immunol  1978  120(6):2027-32  PubMed ID: 307029  
219  Gillis S, Baker PE, Ruscetti FW, Smith KA.  Long-term culture of human antigen-specific cytotoxic T-cell lines  J Exp Med  1978  148(4):1093-8  PubMed ID: 308989   DOI: 10.1084/jem.148.4.1093
2613  Gillis S, Smith, K A  In vitro generation of tumor-specific cytotoxic lymphocytes. Secondary allogeneic mixed tumor lymphocyte culture of normal murine spleen cells.  J Exp Med  1977  146:468-82  PubMed ID: 69003   DOI: 10.1084/jem.146.2.468
1370  Gillis S, Smith, K A  Long term culture of tumour-specific cytotoxic T cells.  Nature  1977  268:154-6  PubMed ID: 145543   DOI: 10.1038/268154a0

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User's Publication
20352  Takenaga K, Koshikawa N, Nagase H.  Intercellular transfer of mitochondrial DNA carrying metastasis-enhancing pathogenic mutations from high- to low-metastatic tumor cells and stromal cells via extracellular vesicles.  BMC Mol Cell Biol  2021  22(1):52  PubMed ID: 34615464   DOI: 10.1186/s12860-021-00391-5
16127  Kobayashi M, Kojima K, Murayama K, Amano Y, Koyama T, Ogama N, Takeshita T, Fukuhara T, Tanaka N.  MK-6, a novel not-α IL-2, elicits a potent antitumor activity by improving the effector to regulatory T cell balance  Cancer Sci   2021  112(11):4478-4489  PubMed ID: 34545658   DOI: 10.1111/cas.15127
12411  Endo K, Kito H, Tanaka R, Kajikuri J, Tanaka S, Elboray EE, Suzuki T, Ohya S.  Possible Contribution of Inflammation-Associated Hypoxia to Increased K 2P 5.1 K + Channel Expression in CD4 + T cells of the Mouse Model for Inflammatory Bowel Disease  J Mol Sci  2019  21(1):38  PubMed ID: 31861667   DOI: 10.3390/ijms21010038
7294  Asama T, Arima TH, Gomi T, Keishi T, Tani H, Kimura Y, Tatefuji T, Hashimoto K.  Lactobacillus kunkeei YB38 from honeybee products enhances IgA production in healthy adults.  J. Appl. Microbiol.  2015  119:818-26  PubMed ID: 26121394   DOI: 10.1111/jam.12889
5334  Mimoto F, Katada H, Kadono S, Igawa T, Kuramochi T, Muraoka M, Wada Y, Haraya K, Miyazaki T, Hattori K.  Engineered antibody Fc variant with selectively enhanced FcγRIIb binding over both FcγRIIa(R131) and FcγRIIa(H131).  Protein Eng Des Sel  2013  26(10):589-98  PubMed ID: 23744091   DOI: 10.1093/protein/gzt022
8048  Kobayashi N, Saito T, Uematsu T, Kishi K, Toba M, Kohda N, Suzuki T.  Oral administration of heat-killed Lactobacillus pentosus strain b240 augments protection against influenza virus infection in mice.  Int. Immunopharmacol.  2011  11:199-203  PubMed ID: 21095259   DOI: 10.1016/j.intimp.2010.11.019
15597  Usui T, Nagumo Y, Watanabe A, Kubota T, Komatsu K, Kobayashi J, Osada H.  Brasilicardin A, a natural immunosuppressant, targets amino Acid transport system L  Chem Biol   2006  13(11):1153-60  PubMed ID: 17113997   DOI: 10.1016/j.chembiol.2006.09.006
2705  Kushida S, Peng, B G, Uchimura E, Kuang M, Huang L, Miwa M, Ohno T  A tumour vaccine of fixed tumour fragments in a controlled-release vehicle with cytokines for therapy of hepatoma in mice.  Dig Liver Dis  2004  36:478-85  PubMed ID: 15285528   DOI: 10.1016/j.dld.2004.02.010
2765  Shou, Zhangfei, Yamada, Kazuya, Kawata, Hiroko, Yokoyama, Osamu, Miyamoto, Kaoru  A mechanism of induction of the mouse zinc-fingers and homeoboxes 1 (ZHX1) gene expression by interleukin-2.  Biochem Biophys Res Commun  2004  314:885-90  PubMed ID: 14741719   DOI: 10.1016/j.bbrc.2003.12.162
2474  Fukushima K, Yamashita K  Interleukin-2 carbohydrate recognition modulates CTLL-2 cell proliferation.  J Biol Chem  2001  276:7351-6  PubMed ID: 11076950   DOI: 10.1074/jbc.M008781200
2560  Ito A, Kataoka, T R, Kim, D K, Koma Y, Lee, Y M, Kitamura Y  Inhibitory effect on natural killer activity of microphthalmia transcription factor encoded by the mutant mi allele of mice.  Blood  2001  97:2075-83  PubMed ID: 11264174   DOI: 10.1182/blood.v97.7.2075
4871  Feng R, Kabayama A, Uchida K, Hoshino H, Miwa M.  Cell-free entry of human T-cell leukemia virus type 1 to mouse cells.  Jpn J Cancer Res  2001  92(4):410-6  PubMed ID: 11346463   DOI: 10.1111/j.1349-7006.2001.tb01110.x
5333  Fukushima K, Hara-Kuge S, Ideo H, Yamashita K.  Carbohydrate recognition site of interleukin-2 in relation to cell proliferation.  J Biol Chem  2001  276(33):31202-8  PubMed ID: 11390392   DOI: 10.1074/jbc.M102789200
11035  T Matsuguchi, K Takagi, T Musikacharoen, Y Yoshikai  Gene expressions of lipopolysaccharide receptors, toll-like receptors 2 and 4, are differently regulated in mouse T lymphocytes  Blood  2000  95(4):1378-85.  PubMed ID: 10666214  
7040  Yu F, Itoyama Y, Kira J, Fujihara K, Kobayashi T, Kitamoto T, Suzumura A, Yamamoto N, Nakajima Y, Goto I.  TNF-beta produced by human T lymphotropic virus type I-infected cells influences the proliferation of human endothelial cells and fibroblasts.  J Immunol  1994  152(12):5930-8  PubMed ID: 8207218  
1773  Xu, B., Aoyama, K. and Ueda, A.  In Vitro Evaluation of Contact Sensitivity to DNCB Using Hapten-Stimulatied In Vitro Interleukin-2 Production by Murine Lymph Node Cells  Toxicology Methods  1993  3:183-194    DOI: 10.3109/15376519309044575

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