Cell No. : Cell Name RCB0624 : SF8425  update : 2023/01/05
Comment			Atomic bomb survivor with breast cancer, different specimen from RCB0621.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Ban, Sadayuki
		Originator	Ban, Sadayuki
		Year of deposit	1991
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Female
		Age at sampling	52 years
		Tissue	skin
		Disease name	atomic bomb survivor
		Classification	normal
		Year of origin	1984
		Lifespan	finite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_K070
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		MEM ALPHA + 15% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Isozyme		LD, NP
		Chromosome mode		46(50)
		STR(human)		OK
Reference information		Reference	2
		User's Publication	0

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Reference

1449  Ban S, Setlow RB, Ezaki H, Hiraoka T, Yamane M, Nishiki M, Dohi K.  X-ray sensitivity of fibroblast cell strains derived from atomic bomb survivors with and without breast cancer.  J Radiat Res  1991  Suppl:330-8  PubMed ID: 1762123   DOI: 10.1269/jrr.32.supplement_330

1442  Ban S, Setlow RB, Bender MA, Ezaki H, Hiraoka T, Yamane M, Nishiki M, Dohi K, Awa AA, Miller RC, et al.  Radiosensitivity of skin fibroblasts from atomic bomb survivors with and without breast cancer.  Cancer Res  1990  50(13):4050-5  PubMed ID: 2354455

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