Cell No. : Cell Name RCB0621 : SF8428  update : 2022/03/18
Comment			Atomic bomb survivor with breast cancer, different specimen from RCB0624
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Ban, Sadayuki
		Originator	Ban, Sadayuki
		Year of deposit	1991
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Female
		Age at sampling	52 years
		Tissue	skin
		Disease name	atomic bomb survivor
		Classification	normal
		Year of origin	1984
		Lifespan	finite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_K071
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		MEM ALPHA + 15% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : once/week, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Virus (HIV)		(-)
		Isozyme		LD, NP
		Chromosome mode		46(50)
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	2
		User's Publication	3

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Reference

1449  Ban S, Setlow RB, Ezaki H, Hiraoka T, Yamane M, Nishiki M, Dohi K.  X-ray sensitivity of fibroblast cell strains derived from atomic bomb survivors with and without breast cancer.  J Radiat Res  1991  Suppl:330-8  PubMed ID: 1762123   DOI: 10.1269/jrr.32.supplement_330

1442  Ban S, Setlow RB, Bender MA, Ezaki H, Hiraoka T, Yamane M, Nishiki M, Dohi K, Awa AA, Miller RC, et al.  Radiosensitivity of skin fibroblasts from atomic bomb survivors with and without breast cancer.  Cancer Res  1990  50(13):4050-5  PubMed ID: 2354455

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User's Publication

20743  Sanada A, Yamada T, Hasegawa S, Ishii Y, Hasebe Y, Iwata Y, Arima M, Sugiura K, Akamatsu H.  Enhanced Type I Collagen Synthesis in Fibroblasts by Dermal Stem/Progenitor Cell-Derived Exosomes.  Biol Pharm Bull  2022  45(7):872-880  PubMed ID: 35786595   DOI: 10.1248/bpb.b21-01084

20841  Miyachi K, Yamada T, Sanada A, Inoue Y, Hasebe Y, Arima M, Iwata Y, Hasegawa S, Sugiura K, Akamatsu H.  Melanin accumulation in dermal stem cells deteriorates their exosome-mediated skin basement membrane construction in solar lentigo.  Exp Dermatol  2022    PubMed ID: 36048560   DOI: 10.1111/exd.14667

3579  Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y.  Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.  Stem Cells  2007  25(7):1610-7  PubMed ID: 17395773   DOI: 10.1634/stemcells.2006-0504