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Cell No. : Cell Name
RCB0595 : HNS5  update : 2025/08/12
CommentSalamander cell line. Fibroblast-like cells.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Hirosaki University
Faculty of Agriculture and Life Science
3 Bunkyo-Cho, Hirosaki-Shi, Aomori-Ken, 036-8561 Japan
Dr.KURO-O Masaki
Fax. +81-172-39-3750
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kuro-o, Masaki
Originator Kuro-o, Masaki
Year of deposit 1991
Animal _salamander < Amphibians
Genus Hynobius
Species vandenburghi
Gender Unknown
Age at sampling embryo
Tissue embryo/fetus, whole
Classification other
Year of origin 1988
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_6654
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 20% D.D.W. + 20% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/2 weeks, Medium Renewal : 2 times/week
Temperature 22 ℃
CO2 concentration 0 %
Freeze medium Medium : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
DNA barcoding OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
23081  Hirotaka SUGAWARA, Takeshi FUJITANI, Shota SEGUCHI, Takuo SAWAHATA, Masahiro NAGANO  Taxonomic Re-examination of the Yamato Salamander Hynobius vandenburghi: Description of a New Species from Central Honshu, Japan  Bulletin of the Kanagawa Prefectural Museum (Natural Science)  2022  no. 51, pp. 47-59    DOI: 10.32225/bkpmnh.2022.51_47
23080  Masafumi MATSUI, Hiroshi OKAWA, Kanto NISHIKAWA, Gen AOKI, Koshiro ETO, Natsuhiko YOSHIKAWA, Shingo TANABE, Yasuchika MISAWA, Atsushi TOMINAGA  Systematics of the Widely Distributed Japanese Clouded Salamander, Hynobius nebulosus (Amphibia: Caudata: Hynobiidae), and Its Closest Relatives  Current Herpetology  2019  38(1): 32–90    DOI: 10.5358/hsj.38.32

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