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Cell No. : Cell Name
RCB0592 : HNS2  update : 2022/03/18
CommentLarger epithelial cells than HNS1A.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
Hirosaki University
Faculty of Agriculture and Life Science
3 Bunkyo-Cho, Hirosaki-Shi, Aomori-Ken, 036-8561 Japan
Dr.KURO-O Masaki
Fax. +81-172-39-3750
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kuro-o, Masaki
Originator Kuro-o, Masaki
Year of deposit 1991
Animal _salamander < Amphibians
Genus Hynobius
Species nebulosus
Gender Unknown
Age at sampling embryo
Tissue embryo/fetus, whole
Classification other
Year of origin 1988
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_6651
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 20% D.D.W. + 20% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 22 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Reference information Reference 0
User's Publication 0

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