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Cell No. : Cell Name
RCB0583 : SF8405  update : 2022/03/18
CommentControl from a nonexposed individual without cancer.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ban, Sadayuki
Originator Ban, Sadayuki
Year of deposit 1991
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 54 years
Tissue skin
Classification normal
Year of origin 1984
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_K064
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Reference information Reference 2
User's Publication 1


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Reference
1449  Ban S, Setlow RB, Ezaki H, Hiraoka T, Yamane M, Nishiki M, Dohi K.  X-ray sensitivity of fibroblast cell strains derived from atomic bomb survivors with and without breast cancer.  J Radiat Res  1991  Suppl:330-8  PubMed ID: 1762123
1442  Ban S, Setlow RB, Bender MA, Ezaki H, Hiraoka T, Yamane M, Nishiki M, Dohi K, Awa AA, Miller RC, et al.  Radiosensitivity of skin fibroblasts from atomic bomb survivors with and without breast cancer.  Cancer Res  1990  50(13):4050-5  PubMed ID: 2354455

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User's Publication
13359  Choong CJ, Okuno T, Ikenaka K, Baba K, Hayakawa H, Koike M, Yokota M, Doi J, Kakuda K, Takeuchi T, Kuma A, Nakamura S, Nagai Y, Nagano S, Yoshimori T, Mochizuki H.  Alternative mitochondrial quality control mediated by extracellular release.  Autophagy  2020    PubMed ID: 33218272



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