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Cell No. : Cell Name
RCB0571 : FVTCT.fl  update : 2024/01/17
CommentFriend leukemia cell line from BALB/c mouse. Virusnon-producing.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ikawa, Yoji
Originator Fieldsteel
Year of deposit 1991
Animal _mouse < Mammals
Strain name BALB/c
Gender Unknown
Tissue spleen
Metastatic ability Yes
Classification cancer
History Fieldsteel?->Ikawa, Y.
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_H732
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 0


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Reference
4783  Black DR, Fieldsteel AH.  Incomplete viral synthesis in Friend leukemia virus-induced reticulum cell sarcomas.  Int J Cancer  1976  17(2):245-53  PubMed ID: 765287   DOI: 10.1002/ijc.2910170213

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