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Cell No. : Cell Name
RCB0570 : GM86.fl  update : 2022/03/17
CommentFriend leukemia cell line from DBA/2 mouse.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ikawa, Yoji
Originator Friend, C.
Year of deposit 1991
Animal mouse < Mammals
Strain name DBA/2
Tissue spleen
Metastastatic ability Yes
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_I000
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 0


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Reference
3684  Todokoro K, Kanazawa S, Amanuma H, Ikawa Y.  Characterization of erythropoietin receptor on erythropoietin-unresponsive mouse erythroleukemia cells.  Biochim Biophys Acta  1988  943(2):326-30  PubMed ID: 2840960   DOI: 10.1016/0005-2736(88)90564-0

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