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Cell No. : Cell Name
RCB0568 : ULF-23  update : 2022/10/19
CommentCold water fish cell line. Cultured at 25 C. Relatively large chromosomes (Mode 23).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Etoh, Hisami
Originator Etoh, Hisami
Year of deposit 1991
Another name ULF-23HU
Animal Mudminnow < Fishes
Strain name Central
Genus Umbra
Species limi
Gender Unknown
Tissue fin, caudal
Classification other
Year of origin 1983
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_5641
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives M199 (20-25mM HEPES) + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 19-25(50) : /20(1),21(5),18(22),23(15),24(8),25(2)
deposit info
lot info
Reference information Reference 3
User's Publication 0

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1381  Park Eun-Ho, Lee Jae-Seong, Yi Ae-Kyung and Etoh Hisami  Fish Cell Line ( ULF-23HU ) Derived from the Fin of the Central Mudminnow ( Umbra limi ) : Suitable Characteristics for Clastogenicity Assay  In Vitro Cell Dev Biol  1989  25:987-994 
1378  Suyama, I. and Etoh, H.  Establishment of A Cell Line from Umbra Lime ( Umbridae ; Pisces )  Invertebrate and Fish Tissue Culture  1988  270-273 
1375  Suyama I, Etoh H  X-ray-induced dicentric yields in lymphocytes of the teleost, Umbra limi.  Mutat Res  1983  107:111-8  PubMed ID: 6828033

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