Cell No. : Cell Name
RCB0566 : CGM1
update : 2024/01/17
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Comment | EB transformed B cell line |
Comment from the depositor | |
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Imai, Takashi
|
Originator |
Olson, V. Maynard
|
Year of deposit |
1991
|
Animal |
_human
< Mammals
|
Genus |
Homo
|
Species |
sapiens
|
Gender |
Male
|
Tissue |
lymphoblast
|
Classification |
transformed
|
History |
Olson, V. Maynard (Washington)
|
Memo_1 |
Mycoplasma was eliminated with MC-210 by the depositor.
|
|
Lifespan |
infinite
|
Morphology |
lymphocyte-like
|
Cellosaurus(Expasy) |
CVCL_7251
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
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Suspension cells
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Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
|
Culture medium |
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RPMI1640 + 10% FBS
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Antibiotics |
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Free
|
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Passage method |
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dilution
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Culture information
|
Passage ratio |
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1 : 10 split
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SC frequency |
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Subculture : 2 times/week
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Temperature |
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37
℃
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CO2 concentration |
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5
%
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Freeze medium |
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Medium + 10% DMSO
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Freezing method |
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Slow freezing
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Mycoplasma/Achoreplasma |
|
(-)
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Virus (HIV) |
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LT 400
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Virus (HTLV-1) |
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(-)
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Isozyme |
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LD, NP
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Chromosome mode |
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45-92(50) : 45(2),46(47),92(1)
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STR(human) |
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OK
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Reference information |
Reference |
1
|
User's Publication |
6
|
User's Publication |
18243
Myouzen K, Kochi Y, Okada Y, Terao C, Suzuki A, Ikari K, Tsunoda T, Takahashi A, Kubo M, Taniguchi A, Matsuda F, Ohmura K, Momohara S, Mimori T, Yamanaka H, Kamatani N, Yamada R, Nakamura Y, Yamamoto K.
Functional variants in NFKBIE and RTKN2 involved in activation of the NF-κB pathway are associated with rheumatoid arthritis in Japanese
PLoS Genet
2012
8(9):e1002949
PubMed ID: 23028356
DOI: 10.1371/journal.pgen.1002949
|
18584
Okada Y, Shimane K, Kochi Y, Tahira T, Suzuki A, Higasa K, Takahashi A, Horita T, Atsumi T, Ishii T, Okamoto A, Fujio K, Hirakata M, Amano H, Kondo Y, Ito S, Takada K, Mimori A, Saito K, Kamachi M, Kawaguchi Y, Ikari K, Mohammed OW, Matsuda K, Terao C, Ohmura K, Myouzen K, Hosono N, Tsunoda T, Nishimoto N, Mimori T, Matsuda F, Tanaka Y, Sumida T, Yamanaka H, Takasaki Y, Koike T, Horiuchi T, Hayashi K, Kubo M, Kamatani N, Yamada R, Nakamura Y, Yamamoto K.
A genome-wide association study identified AFF1 as a susceptibility locus for systemic lupus eyrthematosus in Japanese
PLoS Genet
2012
8(1):e1002455
PubMed ID: 22291604
DOI: 10.1371/journal.pgen.1002455
|
18298
Killen MW, Stults DM, Adachi N, Hanakahi L, Pierce AJ.
Loss of Bloom syndrome protein destabilizes human gene cluster architecture
Hum Mol Genet
2009
18(18):3417-28
PubMed ID: 19542097
DOI: 10.1093/hmg/ddp282
|
13870
Unoki M, Okutsu J, Nakamura Y.
Identification of a novel human gene, ZFP91, involved in acute myelogenous leukemia
Int J Oncol
2003
22(6):1217-23
PubMed ID: 12738986
|
16944
Noguchi T, Ikeda K, Yamamoto K, Yoshida I, Ashiba A, Tsuchiyama J, Shinagawa K, Yoshino T, Takata M, Harada M.
Antisense oligodeoxynucleotides to latent membrane protein 1 induce growth inhibition, apoptosis and Bcl-2 suppression in Epstein-Barr virus (EBV)-transformed B-lymphoblastoid cells, but not in EBV-positive natural killer cell lymphoma cells
Br J Haematol
2001
114(1):84-92
PubMed ID: 11472349
DOI: 10.1046/j.1365-2141.2001.02887.x
|
6246
Ohsawa K, Watanabe Y, Sato H.
Hantaan virus persistently infects marmoset B-lymphoblastoid cell line.
J Vet Med Sci
1995
57(5):931-4
PubMed ID: 8593305
DOI: 10.1292/jvms.57.931
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