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Cell No. : Cell Name
RCB0566 : CGM1  update : 2022/03/17
CommentEB transformed B cell line
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Imai, Takashi
Originator Olson, V. Maynard
Year of deposit 1991
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue lymphoblast
Classification transformed
History Olson, V. Maynard (Washington)
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_7251
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 45-92(50) : 45(2),46(47),92(1)
STR(human) OK
Reference information Reference 1
User's Publication 3


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Reference
1309  Brownstein, B H, Silverman, G A, Little, R D, Burke, D T, Korsmeyer, S J, Schlessinger D, Olson, M V  Isolation of single-copy human genes from a library of yeast artificial chromosome clones.  Science  1989  244:1348-51  PubMed ID: 2544027   DOI: 10.1126/science.2544027

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User's Publication
13870  Unoki M, Okutsu J, Nakamura Y.  Identification of a novel human gene, ZFP91, involved in acute myelogenous leukemia  Int J Oncol  2003  22(6):1217-23  PubMed ID: 12738986  
16944  Noguchi T, Ikeda K, Yamamoto K, Yoshida I, Ashiba A, Tsuchiyama J, Shinagawa K, Yoshino T, Takata M, Harada M.  Antisense oligodeoxynucleotides to latent membrane protein 1 induce growth inhibition, apoptosis and Bcl-2 suppression in Epstein-Barr virus (EBV)-transformed B-lymphoblastoid cells, but not in EBV-positive natural killer cell lymphoma cells  Br J Haematol  2001  114(1):84-92  PubMed ID: 11472349   DOI: 10.1046/j.1365-2141.2001.02887.x
6246  Ohsawa K, Watanabe Y, Sato H.  Hantaan virus persistently infects marmoset B-lymphoblastoid cell line.  J Vet Med Sci  1995  57(5):931-4  PubMed ID: 8593305   DOI: 10.1292/jvms.57.931



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