Cell No. : Cell Name
RCB0561 : T-3-Cl-2-0.fl
update : 2024/01/17
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| Comment | Friend leukemia. Differentiate to erythroblastoid cells with 1.5% DMSO etc. A clone of TSFAT-3.fl |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Ikawa, Yoji
|
| Originator |
Ikawa, Yoji
|
| Year of deposit |
1991
|
| Original cell |
TSFAT-3, T-3-cl-2
|
| Animal |
_mouse
< Mammals
|
| Strain name |
DDD
|
| Gender |
Unknown
|
| Tissue |
spleen
|
| Classification |
cancer
|
| Memo_1 |
Mycoplasma was eliminated with MC-210 by the depositor.
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_4313
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| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
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Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 + 10% FBS
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| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
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Culture information
|
Passage ratio |
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1 : 10 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
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| Reference information |
Reference |
9
|
| User's Publication |
0
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| Reference |
|
4782
Aisaki K, Kanno H, Oyaizu N, Hara Y, Miwa S, Ikawa Y.
Apoptotic changes precede mitochondrial dysfunction in red cell-type pyruvate kinase mutant mouse erythroleukemia cell lines.
Jpn J Cancer Res
1999
90(2):171-9
PubMed ID: 10189887
DOI: 10.1111/j.1349-7006.1999.tb00730.x
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3684
Todokoro K, Kanazawa S, Amanuma H, Ikawa Y.
Characterization of erythropoietin receptor on erythropoietin-unresponsive mouse erythroleukemia cells.
Biochim Biophys Acta
1988
943(2):326-30
PubMed ID: 2840960
DOI: 10.1016/0005-2736(88)90564-0
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4779
Kasahara M, Hinkle PC, Ikawa Y, Amanuma H.
Decrease in glucose transport activity of Friend erythroleukemia cell caused by dimethylsulfoxide, a differentiation-inducing reagent.
Biochim Biophys Acta
1986
856(3):615-23
PubMed ID: 3457604
DOI: 10.1016/0005-2736(86)90155-0
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4768
Matsugi T, Amanuma H, Ikawa Y.
Presence of a novel spleen focus-forming virus-specific glycoprotein (gp51) in a Friend leukemia cell line and its decrease during erythrodifferentiation.
Gan
1983
74(4):509-16
PubMed ID: 6628903
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4776
Obinata M, Uchiyama Y, Kameji R, Ikawa Y.
Analysis on the molecular mechanism of globin gene expression in phenotypic variants of cultured Friend leukemia cells.
Leuk Res
1981
5(2):129-40
PubMed ID: 7242141
DOI: 10.1016/0145-2126(81)90071-0
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1372
Ikawa Y, Aida M, Inoue Y
Isolation and characterization of high and low differentiation-inducible Friend leukemia lines.
Gann
1976
67:767-70
PubMed ID: 1071084
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4772
Ross J, Ikawa Y, Gielen J, Packman S, Leder P.
Induction of globin mRNA in Friend leukemia virus-infected cells and its presence in viral 60S RNA.
Bibl Haematol
1975
40:205-20.
PubMed ID: 1172437
DOI: 10.1159/000397535
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4771
Ross J, Ikawa Y, Leder P.
Globin messenger-RNA induction during erythroid differentiation of cultured leukemia cells.
Proc Natl Acad Sci U S A
1972
69(12):3620-3
PubMed ID: 4509323
DOI: 10.1073/pnas.69.12.3620
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4770
Ikawa Y, Sugano H.
An ascites tumor derived from early splenic lesion of Friend's disease: a preliminary report.
Gan
1966
57(6):641-3
PubMed ID: 5973415
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