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Cell No. : Cell Name
RCB0561 : T-3-Cl-2-0.fl  update : 2022/12/14
CommentFriend leukemia. Differentiate to erythroblastoid cells with 1.5% DMSO etc. A clone of TSFAT-3.fl
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ikawa, Yoji
Originator Ikawa, Yoji
Year of deposit 1991
Original cell TSFAT-3, T-3-cl-2
Animal _mouse < Mammals
Strain name DDD
Gender Unknown
Tissue spleen
Classification cancer
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_4313
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 9
User's Publication 0

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4782  Aisaki K, Kanno H, Oyaizu N, Hara Y, Miwa S, Ikawa Y.  Apoptotic changes precede mitochondrial dysfunction in red cell-type pyruvate kinase mutant mouse erythroleukemia cell lines.  Jpn J Cancer Res  1999  90(2):171-9  PubMed ID: 10189887   DOI: 10.1111/j.1349-7006.1999.tb00730.x
3684  Todokoro K, Kanazawa S, Amanuma H, Ikawa Y.  Characterization of erythropoietin receptor on erythropoietin-unresponsive mouse erythroleukemia cells.  Biochim Biophys Acta  1988  943(2):326-30  PubMed ID: 2840960   DOI: 10.1016/0005-2736(88)90564-0
4779  Kasahara M, Hinkle PC, Ikawa Y, Amanuma H.  Decrease in glucose transport activity of Friend erythroleukemia cell caused by dimethylsulfoxide, a differentiation-inducing reagent.  Biochim Biophys Acta  1986  856(3):615-23  PubMed ID: 3457604   DOI: 10.1016/0005-2736(86)90155-0
4768  Matsugi T, Amanuma H, Ikawa Y.  Presence of a novel spleen focus-forming virus-specific glycoprotein (gp51) in a Friend leukemia cell line and its decrease during erythrodifferentiation.  Gan  1983  74(4):509-16  PubMed ID: 6628903  
4776  Obinata M, Uchiyama Y, Kameji R, Ikawa Y.  Analysis on the molecular mechanism of globin gene expression in phenotypic variants of cultured Friend leukemia cells.  Leuk Res  1981  5(2):129-40  PubMed ID: 7242141   DOI: 10.1016/0145-2126(81)90071-0
1372  Ikawa Y, Aida M, Inoue Y  Isolation and characterization of high and low differentiation-inducible Friend leukemia lines.  Gann  1976  67:767-70  PubMed ID: 1071084  
4772  Ross J, Ikawa Y, Gielen J, Packman S, Leder P.  Induction of globin mRNA in Friend leukemia virus-infected cells and its presence in viral 60S RNA.  Bibl Haematol  1975  40:205-20.  PubMed ID: 1172437   DOI: 10.1159/000397535
4771  Ross J, Ikawa Y, Leder P.  Globin messenger-RNA induction during erythroid differentiation of cultured leukemia cells.  Proc Natl Acad Sci U S A  1972  69(12):3620-3  PubMed ID: 4509323   DOI: 10.1073/pnas.69.12.3620
4770  Ikawa Y, Sugano H.  An ascites tumor derived from early splenic lesion of Friend's disease: a preliminary report.  Gan  1966  57(6):641-3  PubMed ID: 5973415  

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